Resultados de investigación

Under a Creative Commons license BY-NC-ND 4.0 Deed., Synthesis of cellulose nanocrystals: Cellulose nanocrystals (CNCs) were synthesized following the protocol described in two previous publications.[1,2] 10 g of microcrystalline cellulose (MCC) (Sigma-Aldrich, ref 310697) were dispersed in 45 ml of ultrapure water using an ultrasound bath (45 kHz) for 5 min. Then, 45 ml of H2SO4 98 wt% (Labkem, ref SUAC-00A) were added dropwise to the mixture (externally cooled in an ice/water bath at 0 °C) reaching a final H2SO4 concentration of 64 wt%. This addition was performed fast (less than 5 min) and with magnetic stirring to obtain type I CNCs or slow (longer than 100 min) and with high shear mixing to obtain type II CNCs.[1] After that, the mixture was put in a heating plate at 70°C for 10 minutes for type I CNCs or at ambient temperature for 1 hour for type II CNCs. The reaction was stopped by diluting in 1 liter of ultrapure water at 4°C, also to increase the pH, and the mixture was left decanting overnight at 4°C. The bottom sediment was dialyzed against ultrapure water until a neutral pH was achieved, using specific dialysis membranes (Merck, average flat width 33 mm, ref D9652-100FT). The neutralized dispersions were subjected to centrifugation/re-dispersion cycles at 9000 rpm (9327 rcf) for 1 min to keep only the nanocrystals. The average mass yield and the concentration (Table S1) were determined by lyophilization of 3 aliquots of 30 mL of the CNCs dispersions. The hydrodynamic radii, polydispersity, and ζ-potential were measured using a Malvern Nano ZS instrument, and the mean values of these parameters are presented in Table S1. The CNCs in solid were measured by a Bruker D8 Advance X-Ray diffractometer using a Cu tube as the X-ray source (λ Cu Kα = 1.54 Å), a tube voltage of 40 kV, and a current of 40 mA. The X-Ray diffraction patterns of the prepared CNCs (Fig. S1) confirmed the different presence of the characteristic planes of type I or type II cellulose.--, Synthesis of cellulose nanocrystals: Table S1. Mean values of the characterization results of type I and type II CNCs dispersions. Fig. S1. X-ray diffraction profiles of type I (red) and type II (blue) CNCs. Surface resistivity of n-type thermoelectric cotton textiles as a function of the number of dip-coating cycles: Fig. S2. Surface resistivity (Rsh) of nanocomposite textiles from 1 to 10 immersion cycles with equivalent inks, but different immersion methodology: regular (red) and using a complementary ultrasounds bath treatment for 5 min (black). Measured with an in-line 4-point probe configuration. Error bars show standard deviation of at least 3 repetitions. SEM images of the surface of original and washed CWF@CNF textile samples: Fig. S3. SEM images of the surface of original and washed CWF@CNF textile samples at different magnifications (a) CWF@CNF, (b) CWF@CNF W30 and (c) CWF@CNF W45. Model proposed for describing the nonlinear Seebeck of CNFs and CWF@CNF: The model presented in this work represents the combination of two physical mechanisms occurring in parallel: S(T)=S_met (T)+S_imp (T) (1). Power factor and estimative figure of merit of unwashed and washed samples and CNFs: Table S2. Thermoelectrical properties of dip-coated textiles and carbon nanofibers. References., Financial support from Spanish MCIN/AEI under projects PID2019-104272RB-C51/AEI/10.13039/501100011033 and PID2020-120439RA-I00, from Spanish CSIC (PIE iniciación ref. 202280I007), as well as from Gobierno de Aragón (DGA, Grupo Reconocido DGA-T03_23R) is acknowledged. Vícto Calvo thanks the DGA for funding his PhD contract (Ref. CUS/581/2020). Antonio J. Paleo gratefully acknowledges support from FCT-Foundation for Science and Technology by the “plurianual” 2020–2023 Project UIDB/00264/2020, and European COST Action EsSENce CA19118 for its support with the Short Term Scientific Mission (STSM) grant E-COST-GRANT-CA19118-0ed3a197 at IPF (Dresden). E. Muñoz acknowledges funding from ANID ANILLO ACT/192023 and Fondecyt No 1230440., Peer reviewed

[Description of methods used for collection/generation of data] Los datos batimétricos se obstuvieron con ecosonda multihaz en las campañas ESPACE02 y MOSAICO0509. Los persiles sísmicos de alta resolución se obtuvieron en la campaña MOSAICO0908 con Uniboom seismic.Los testigos de sedimento se obtuvieron en la campaña MOSAICO 1108., [Methods for processing the data] Los datos batimétricos de la campaña ESPACE02 se procesaron con el software Neptune y los obtenidos en la campaña MOSAICO0509 se procesaron con el software Caraibes. Posteriormente, los datos batimétricos se integraron en un sistema de información geográfica. Los datos sísmicos se procesaron e interpretaron con el software Kingdom™ Suite. A las muestras de sedimentos se realizaron análisis granulométricos y dataciones con radiocarbono., [EN] The formation and development of a small Mediterranean deltaic system are investigated through a primary seismic stratigraphic interpretation of a high-resolution seismic profile network, combined with multiple bathymetric data (including multibeam bathymetric imagery) and collated with shallow sediment cores collected with a vibro-corer device. The submarine delta of the Adra River is divided into a basal patchy seismic unit and five wedge-shaped younger seismic units that are related to the Holocene highstand stabilization. Limited age control indicates that the two uppermost seismic units are very recent, most likely related to a dearth of fluvial fluxes led by channel deviations and by sediment retention. The formation of the three older seismic units is correlated to three humid periods during the middle Holocene, late Holocene and Little Ice Age, under a general context of progressive aridification of southeastern Iberia. The stacking patterns and spatial distribution of individual seismic units document a history of episodic progradation of successive prodeltaic lobes, with a long-term evolution mediated by climatically-induced changes in the river basin and more recent anthropogenic interventions. Overall, the prodeltaic system registers the complete modification of a deltaic system that evolves from a fluvial-dominated delta to recent wave-dominated wedges. In between, the deltaic system exhibits a progressive asymmetric character, due to the instauration of Atlantic waters on the shelf and their subsequent eastward redistribution. The Adra deltaic system is proposed as an outstanding example of a small deltaic system that reacts almost immediately to the complex interaction between natural changes in the system and anthropogenic interventions in the drainage basin., [ES] La formación y la evolución de un pequeño sistema deltaico mediterráneo se ha estudiado mediante la interpretación estratigráfica de una red de perfiles sísmicos de alta resolución, combinada con múltiples datos batimétricos (incluidas datos de batimetria multihaz) y cotejada con testigos de sedimentos someros recogidos con vibro-corer. En el delta submarino del río Adra se diferencia una unidad sísmica basal y cinco unidades sísmicas más jóvenes en forma de cuña que están relacionadas con la estabilización del alto nivel del Holoceno. A partir de las dataciones realizadas se observa que las dos unidades sísmicas superiores son muy recientes, muy probablemente relacionadas con una disminución en los aportes fluviales provocada por las desviaciones del cauce y por la retención de sedimentos. La formación de las tres unidades sísmicas más antiguas se correlaciona con tres periodos húmedos durante el Holoceno medio, el Holoceno tardío y la Pequeña Edad de Hielo, en un contexto general de progresiva aridificación del sureste de Iberia. Los patrones de sedimentación y la distribución espacial de las unidades sísmicas indican una progradación episódica de sucesivos lóbulos prodeltaicos , con una evolución a largo plazo en la cuenca fluvial mediada por cambios climáticos y más recientemente por intervenciones antropogénicass. En conjunto, el sistema prodeltaico registra la modificación de un sistema deltaico que evoluciona en principio como un delta dominado por flujos fluviales hasta cuñas recientes dominadas por el oleaje. Entre medias, el sistema deltaico muestra un carácter asimétrico progresivo, debido a la influencia de las aguas atlánticas en la plataforma y su posterior redistribución hacia el este. El sistema deltaico del río Adra se propone como ejemplo de un pequeño sistema deltaico que reacciona de forma casi inmediata a la compleja interacción entre los cambios naturales del sistema y las intervenciones antropogénicas en la cuenca de drenaje., "Modelado, simulación numérica y análisis del transporte de sedimentos en los abanicos submarinos de los ríos de Andalucía oriental” (acrónimo: MOSAICO), financiado por la Junta de Andalucía (http://anamat.cie.uma.es/mosaico). "Estudio sistemático de la plataforma continental y talud superior españoles (Proyecto ESPACE)", financiado por la Secretaría General de Pesca. Ministerio de Agricultura, Pesca y Alimentación., Peer reviewed

The dataset is made available under the Open Database License. Any rights in individual contents of the database are licensed under the Database Contents License. Please, read the full ODbL 1.0 license text for the exact terms that apply. Users of the dataset are free to: Share: copy, distribute and use the database, either commercially or non-commercially. Create: produce derivative works from the database. Adapt: modify, transform and build upon the database. Under the following conditions: Attribution: You must attribute any public use of the database, or works produced from the database. For any use or redistribution of the database, or works produced from it, you must make clear to others the license of the original database. Share-Alike: If you publicly use any adapted version of this database, or works produced from an adapted database, you must also offer that adapted database under the ODbL., This work was supported by the Severo Ochoa Excellence Program CEX2021-001189-S, grant PID2021-125858OB-100, and a Juan de la Cierva Incorporación grant (IJC2020-042749-I) funded by MCIN/AEI/ 10.13039/501100011033, “ERDF A way of making Europe” and ‘NextGenerationEU’/PRTR’. CR-P holds a predoctoral grant (ACIF/2021/364) funded by Conselleria d’Educació (Generalitat Valenciana, Comunitat Valenciana, Spain)., With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX 2021-001189-S), Peer reviewed

[Description of methods used for collection/generation of data] We collected birds infected by Plasmodium cathemerium and Plasmodium relictum and uninfected birds. We collected mosquito larvae that were raised in the lab. Adult mosquitoes were divided in 3 groups allowed them to feed overnight on a P. relictum-infected bird, a P. cathemerium-infected bird and an uninfected control bird. For transcriptome analyses, we processed mosquitoes at three time points after exposure, 24 hours post-infection (hpi), 10 days post-infection (dpi) and 21 dpi. At each time point, we created pools of 5 mosquitoes of each infection status capturing the mosquitoes alive and immediately transferring them to dry ice. We preserved the mosquitoes at -80ºC until RNA extractions were carried out. We collected a total of 36 samples including controls (4 pools x 3 time points x 3 conditions). We extracted RNA and DNA from pools of 5 mosquitoes using TRIzol® (Invitrogen, Carlsbad, CA, USA) followed by column purification using RNeasy mini kit® (QIAGEN, Hilden, Germany). RNA samples were submitted to the Polo d’Innovazione di Genomica, Genetica e Biologia, Siena (Italy) where library preparation and sequencing were carried out., [Methods for processing the data] Illumina sequencing and RNAseq pipeline available with the published paper., Metadata describing the experimental conditions of an RNA seq experiment that aims to analyse the differential gene expression of Culex pipiens when exposed to either Plasmodium relictum or Plasmodium cathemerium. The data includes the information of the corresponding project published at the European Nucleotide Archive (https://www.ebi.ac.uk/ena/browser/view/PRJEB41609), sample IDs and the links to the generated fasta files., This publication was supported by the project Research Infrastructures for the control of vector-borne diseases (Infravec2), which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 731060., Peer reviewed

1. Description of methods used for collection/generation of data: The flower reflectance spectra were made with USB-2000 and Jaz A1465 Ocean Optics spectrophotometers (Duiven, The Netherlands) equipped with a top sensor system deuterium-halogen standardized light source, DT-MINI-GS-2 and DH-2000 lamps, respectively, and a coaxial fibber cable (QR-400-7-UV-VIS-BX; Ocean Optics). Reflectance corresponds to the proportion of a standard white reference tile (WS-1-SS; Ocean Optics). For all measurements, we kept the distance between the petals and the measuring probe constant and with an angle of illumination and reflection fixed at 45°. Spectra data were processed with OceanView software (version 2.0.8; Ocean Optics) and calculated in 5-nm wide spectral intervals in the range of 300–700 nm. 2. Methods for processing the data: We used the ‘getspec’ function of the ‘pavo’ R-package to load the colour spectral data between 300 and 700 nm of each flower. To reduce noise, the data was smoothed with a span of 0.25 and negative values were corrected by setting the minimum value to zero and scaling other values accordingly (‘procspec’ function). 3. Instrument- or software-specific information needed to interpret/reproduce the data, please indicate their location: USB-2000 and Jaz A1465 Ocean Optics spectrophotometers (Duiven, The Netherlands) equipped with a top sensor system deuterium-halogen standardized light source, DT-MINI-GS-2 and DH-2000 lamps, respectively, and a coaxial fibber cable (QR-400-7-UV-VIS-BX; Ocean Optics). Reflectance corresponds to the proportion of a standard white reference tile (WS-1-SS; Ocean Optics). Spectra data were processed with OceanView software (version 2.0.8; Ocean Optics). 4. Standards and calibration information, if appropriate: Reflectance corresponds to the proportion of a standard white reference tile (WS-1-SS; Ocean Optics)., 1. Many insular plant species inhabiting different archipelagos worldwide present typical ornithophilous floral traits (e.g., copious nectar, red-orange colours), but most of them are visited by insectivorous/granivorous birds and lizards, which act as generalist pollinators. Oceanic islands promote these ecological interactions mainly due to the scarcity of arthropods. Our goal is to understand how these generalist interactions contribute to the shift of floral traits from entomophily (mainland) to ornithophily or saurophily (island), where specialist nectar-feeding birds have not inhabited. 2. We used the well-known pollination interactions occurring in the Canary Islands to evaluate two proposed ecological hypotheses, bee-avoidance or bird-attraction, explaining evolutionary transitions of floral traits. Specifically, we studied the flower colour conspicuousness of bird-pollinated Canarian species visited by birds and lizards with their closest relatives from the mainland mainly visited by bees. We analysed the chromatic contrast of flower colours using visual models of bees, birds, and lizards and the achromatic contrast in visual models of bees. We also compared reflectance spectra marker points of flowers with available spectral discrimination sensitivities of bees and birds. 3. Using a phylogenetically corrected framework of independent plant lineages, our results revealed that bird-pollinated Canarian species showed lower chromatic contrast according to bees and lizard visual models than their mainland relatives, but similar chromatic contrast for bird vision. In addition, reflectance spectra marker points of the Canarian species were displaced to the longest wavelengths, far from the wavelengths of maximum discrimination of bees, but close to birds. 4. We conclude that the avoidance of bees would be a primary ecological strategy explaining the evolutionary transitions of flower colours from melittophily to ornithophily. The lower conspicuousness of bird-pollinated Canarian flowers in lizards is perhaps a side effect of the bee-avoidance strategy rather than an independent evolutionary strategy. Together, these findings provide insights into how vertebrate generalist pollinators can also lead to divergence of floral traits in insular habitats, but also in other arthropod-poor habitats., Fundación CajaCanarias/La Caixa (2022CLISA29), Spanish Ministry of Science and Innovation (PID2020-116222GB-100)., Flower reflectance data, Peer reviewed

[Description of methods used for collection/generation of data] The data were acquired during diverse missions executed by the WeLASER robot., This database presents diverse logs of the results of the tests developed with the WeLASER robot, over a period of 9 months, between January and September 2023, in 3 diverse experimental farms in Spain, The Netherlands and Denmark, in real crop fields, executing autonomous navigation operations. The logs include data collected by the robot's various guidance controllers, including the spiral controller and the lateral controller. The dataset also includes the log information already extracted, in two .mat files, and a MATLAB script that allows graphing and analyzing said information. This for the purpose of promoting transparency, reproducibility, and the sharing of our research findings., This dataset is part of a the WeLASER project that has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 101000256., The dataset is made up of: (a) a folder that includes all the logs of the different controllers executing various missions; (b) two .mat files that include the information extracted from said logs; and (c) a matlab script that allows graphing and analyzing the information contained in the two .mat files., Peer reviewed

-Data were generetaed from February to August 2022._ -vcab_single,xml and kTsweek.xml are the input files for VCAbuilder and kTsweep, executables of the almaBTE package. The folder 'KNbO3' contains the force constants comouted wuth DFT and other files necessary to run the calculations. KNbO3_22_22_22_100_400.Tsweep is the output of kTsweep._ -Data were generetaed as detailed in the related article._ -Files can be opened and read with a simple text editor. The almaBTE code (freely available at https://almabte.bitbucket.io/) is needed to carry out the thermal conductivity calcualtions._, In this project we computed the thermal conductivity of three different crystal phases of a ferroelectric perovskite, KNO3. The R3c and the Amm2 are polar phase that are stable at low temperature and room temperature, respectively. The Pm-3m phase is stabilized by photoexcitation (which we effectively simulate with a larger broadening of the Fermi-Dirac distribution, i.e. 0.50 vs 0.01). Each folder contains the files necessary to carry out a thermal conductivity calculations with almaBTE (freely available at https://almabte.bitbucket.io/). The force constants were obtained with the DFT package VASP, as detailed in the related article., Thermal conductivity of (a) the Amm2 phase with a Fermi-Dirac broadening of 0.01; (b) the Pm-3m phase with a Fermi-Dirac broadening of 0.50; (3) the R3m phase with a Fermi-Dirac broadening of 0.01, Peer reviewed

The divergent reactivity of the cationic iridium complex [(η5-C5Me5)IrCl(PMe2ArDipp2)]+ (ArDipp2 = C6H3–2,6-(C6H3–2,6-iPr2)2) toward organolithium and Grignard reagents is described. The noninnocent behavior of the Cp* ligand, a robust spectator in the majority of stoichiometric and catalytic reactions, was manifested by its unforeseen electrophilic character toward organolithium reagents LiMe, LiEt, and LinBu. In these unconventional transformations, the metal center is only indirectly involved by means of the Ir(III)/Ir(I) redox cycle. In the presence of less nucleophilic organolithium reagents, the Cp* ligand also exhibits noninnocent behavior undergoing facile deprotonation, which is also concomitant with the reduction of the metal center. In turn, the weaker alkylating agents EtMgBr and MeMgBr effectively achieve the alkylation of the metal center. These reactive iridium(III) alkyls partake in subsequent reactions: while the ethyl complex undergoes β-H elimination, the methyl derivative releases methane by a remote C–H bond activation. Computational studies, including the quantum theory of atoms in molecules (QTAIM), support that the preferential activation of the non-benzylic C–H bonds takes place via sigma-bond metathesis., Peer reviewed

3 pages. -- This PDF file includes: Figure S1. Figure S1. Effect of tunicamycin on Orai1 plasma membrane expression; Table 1. Table S1. Tunicamycin attenuates Orai1 and STIM1 protein expression in MCF10A and MDA-MB-231 cells., Peer reviewed

9 pages. -- Encoding visualization. -- Figure S1-S14. -- Table S1. Cell type, number of samples detail, and percentage of samples above or below the encoding-based threshold of Melanoma dataset during the testing phase., Peer reviewed