BUSCADOR RECOLECTA

CORA.Repositori de Dades de Recerca

doi:10.34810/data163

This dataset contains the raw data of the experiments described in the paper "Transplanting rejuvenated blood stem cells extends lifespan of aged immunocompromised mice" by Montserrat-Vazquez, Noelle, Matteini et al., as well as the code used to analyse the single-cell RNA sequencing (scRNA-seq) data, the raw count matrices and all the output obtained from running the code. scRNA-seq was applied on sorted Lin- Sca1+ cKit+ (LSK) cells from the bone marrow of young, aged and aged CASIN-treated C57Bl6 mice and the raw FASTQ files can be found under the GEO accession number GSE197070. The code is in an easily reproducible format, as Rmarkdown and jupyter notebooks that contain comments that describe all the steps in the analysis. To start reproducing it, download all the files, open the .Rproj file in an Rstudio session and start following the instructions in the file "LSK_scRNAseq_data_analysis.Rmd".