Dataset.
Additional file 2 of An amino acid transporter subunit as an antibody–drug conjugate target in colorectal cancer
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360658
Digital.CSIC. Repositorio Institucional del CSIC
- Montero, Juan Carlos
- Carmen, Sofía del
- Abad, Mar
- Sayagués, José María
- Barbáchano, Antonio
- Fernández-Barral, Asunción
- Muñoz Terol, Alberto
- Pandiella, Atanasio
Additional file 2: Supplementary Fig. 2. A) Internalization of the anti-CD98hcECTO antibody in SW480 cells, analyzed by immunofluorescence. Scale bar = 25 μm. The cells were seeded on coverslips and treated with 10 nM of anti-CD98hcECTO for the times indicated. The images at the right correspond to magnifications of a cell present in the images obtained at 24 h. The colocalization of CD98hc and LAPM1 is show in the merged images. B) Preparation of the antibody–drug conjugate targeting CD98hc. The coupling of DM1 to the anti-CD98hcECTO antibody was evaluated by Western blot using an anti-DM1 antibody. Twenty nanograms of anti-CD98hcECTO-DM1, the nude anti-CD98hcECTO, trastuzumab or T-DM1 were used to detect DM1 (upper panel) and the total amount of protein was evaluated by stain-free blot (lower image). Trastuzumab and T-DM1 were used as a negative and positive controls. C-E) FACS analyses in HT29 (C), cells dissociated from patient PDX BT6224 (D) and human tumoral organoid #48 (E) using anti-CD98hc-DM1 as primary antibody. The red histogram correspond to signals from cells incubated with the secondary antibody alone, whereas the blue histograms represent the fluorescence due to the expression of CD98hc., Instituto de Salud Carlos III Consejo Superior de Investigaciones Científicas Junta de Castilla y León ALMOM ACMUMA UCCTA CRIS Cancer Foundation Agencia Estatal de Investigación Consejo Superior de Investigaciones Cientificas (CSIC), Peer reviewed
DOI: http://hdl.handle.net/10261/360658
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360658
HANDLE: http://hdl.handle.net/10261/360658
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360658
Ver en: http://hdl.handle.net/10261/360658
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360658
No hay resultados en la búsqueda
No hay resultados en la búsqueda
×
1 Versiones
1 Versiones
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360658
Dataset. 2023
ADDITIONAL FILE 2 OF AN AMINO ACID TRANSPORTER SUBUNIT AS AN ANTIBODY–DRUG CONJUGATE TARGET IN COLORECTAL CANCER
Digital.CSIC. Repositorio Institucional del CSIC
- Montero, Juan Carlos
- Carmen, Sofía del
- Abad, Mar
- Sayagués, José María
- Barbáchano, Antonio
- Fernández-Barral, Asunción
- Muñoz Terol, Alberto
- Pandiella, Atanasio
Additional file 2: Supplementary Fig. 2. A) Internalization of the anti-CD98hcECTO antibody in SW480 cells, analyzed by immunofluorescence. Scale bar = 25 μm. The cells were seeded on coverslips and treated with 10 nM of anti-CD98hcECTO for the times indicated. The images at the right correspond to magnifications of a cell present in the images obtained at 24 h. The colocalization of CD98hc and LAPM1 is show in the merged images. B) Preparation of the antibody–drug conjugate targeting CD98hc. The coupling of DM1 to the anti-CD98hcECTO antibody was evaluated by Western blot using an anti-DM1 antibody. Twenty nanograms of anti-CD98hcECTO-DM1, the nude anti-CD98hcECTO, trastuzumab or T-DM1 were used to detect DM1 (upper panel) and the total amount of protein was evaluated by stain-free blot (lower image). Trastuzumab and T-DM1 were used as a negative and positive controls. C-E) FACS analyses in HT29 (C), cells dissociated from patient PDX BT6224 (D) and human tumoral organoid #48 (E) using anti-CD98hc-DM1 as primary antibody. The red histogram correspond to signals from cells incubated with the secondary antibody alone, whereas the blue histograms represent the fluorescence due to the expression of CD98hc., Instituto de Salud Carlos III Consejo Superior de Investigaciones Científicas Junta de Castilla y León ALMOM ACMUMA UCCTA CRIS Cancer Foundation Agencia Estatal de Investigación Consejo Superior de Investigaciones Cientificas (CSIC), Peer reviewed
There are no results for this search
1106