Dataset.

Hepatic proliferation dynamics and early establishment of a 1:9 BEC:hepatocyte ratio during embryonic development [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
  • Unterweger, Iris. A.
  • Klepstad, Julie
  • Hannezo, Edouard
  • Lundegaard, Pia R.
  • Trusina, Ala
  • Ober, Elke A.
(A) Approximately 5 μm projection of a 72-hpf liver expressing tp1:H2B-mCherry (BEC), stained for Hnf4a (hepatocytes) and EdU (proliferating cells). Yellow and white arrowheads highlight proliferating BECs and hepatocytes, respectively (N = 2, n = 10 livers). (B) Graph showing the proportion of EdU+ proliferating hepatocytes and BECs over time (N = 2, n ≥ 8). (C, D) Graph showing hepatocyte (C) and BEC (D) cell numbers during development (N = 4, n ≥ 12 livers). (E) Quantification of total liver volume during development determined in embryos in BABB (N = 4, n ≥ 12 livers). (F) Maximum projection (20 μm z-stacks) of a 48-hpf liver expressing tp1:H2B-mCherry (BEC) and stained for Hnf4ɑ (hepatocyte). (G) Relative distribution of BECs and hepatocytes during development from 48 to 144 hpf (N = 4, n ≥ 12 livers). (B-E) Different shape data points indicate different experiments. The numerical values that were used to generate the graphs in (B-E, G) can be found in S1 Data. BEC, biliary epithelial cell; EdU, 5-ethynyl-2′-deoxyuridine; hpf, hours post fertilization., Peer reviewed
 
DOI: http://hdl.handle.net/10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005

HANDLE: http://hdl.handle.net/10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005
 
Ver en: http://hdl.handle.net/10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005
Dataset. 2023

HEPATIC PROLIFERATION DYNAMICS AND EARLY ESTABLISHMENT OF A 1:9 BEC:HEPATOCYTE RATIO DURING EMBRYONIC DEVELOPMENT [DATASET]

Digital.CSIC. Repositorio Institucional del CSIC
  • Unterweger, Iris. A.
  • Klepstad, Julie
  • Hannezo, Edouard
  • Lundegaard, Pia R.
  • Trusina, Ala
  • Ober, Elke A.
(A) Approximately 5 μm projection of a 72-hpf liver expressing tp1:H2B-mCherry (BEC), stained for Hnf4a (hepatocytes) and EdU (proliferating cells). Yellow and white arrowheads highlight proliferating BECs and hepatocytes, respectively (N = 2, n = 10 livers). (B) Graph showing the proportion of EdU+ proliferating hepatocytes and BECs over time (N = 2, n ≥ 8). (C, D) Graph showing hepatocyte (C) and BEC (D) cell numbers during development (N = 4, n ≥ 12 livers). (E) Quantification of total liver volume during development determined in embryos in BABB (N = 4, n ≥ 12 livers). (F) Maximum projection (20 μm z-stacks) of a 48-hpf liver expressing tp1:H2B-mCherry (BEC) and stained for Hnf4ɑ (hepatocyte). (G) Relative distribution of BECs and hepatocytes during development from 48 to 144 hpf (N = 4, n ≥ 12 livers). (B-E) Different shape data points indicate different experiments. The numerical values that were used to generate the graphs in (B-E, G) can be found in S1 Data. BEC, biliary epithelial cell; EdU, 5-ethynyl-2′-deoxyuridine; hpf, hours post fertilization., Peer reviewed




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