Dataset.

Cytokine profile and phagocytic capacity of Ndufs4−/− macrophages [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352276
Digital.CSIC. Repositorio Institucional del CSIC
  • Serrano-Lorenzo, Pablo
  • Gobelli, Dino
  • Garrido-Moraga, Rocío
  • Esteban-Amo, María J.
  • López-López, José R.
  • Orduña, Antonio
  • Fuente, Miguel A. de la
  • Martín, Miguel Ángel
  • Simarro-Grande, María
(A, B) Parental (Par), and Ndufs4−/− RAW 264.7 cells were left untreated or treated with LPS (100 ng/ml). Supernatants were collected at 8 hours for measurement of cytokine concentrations by ELISA (A). Cells were collected at 4 hours for quantification of cytokine transcripts using real-time PCR (expressed as fold increases versus untreated parental cells) (B). (C) Representative flow cytometry plots (left) showing phagocytosis of FITC labeled heat killed E. coli (HKEC) and bar graph representing % of fluorescent cells and the MFI values (right). Ctrl, control. Ns, not significant; *, P <0.05; **, P <0.01; ***, P<0.005; ****, P<0.001. Each point represents a biological replicate. In all experiments, 4 to 5 different Ndufs4−/− clones were used. Data are shown as the mean ± SEM., Peer reviewed
 
DOI: http://hdl.handle.net/10261/352276
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352276

HANDLE: http://hdl.handle.net/10261/352276
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352276
 
Ver en: http://hdl.handle.net/10261/352276
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352276

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352276
Dataset. 2023

CYTOKINE PROFILE AND PHAGOCYTIC CAPACITY OF NDUFS4−/− MACROPHAGES [DATASET]

Digital.CSIC. Repositorio Institucional del CSIC
  • Serrano-Lorenzo, Pablo
  • Gobelli, Dino
  • Garrido-Moraga, Rocío
  • Esteban-Amo, María J.
  • López-López, José R.
  • Orduña, Antonio
  • Fuente, Miguel A. de la
  • Martín, Miguel Ángel
  • Simarro-Grande, María
(A, B) Parental (Par), and Ndufs4−/− RAW 264.7 cells were left untreated or treated with LPS (100 ng/ml). Supernatants were collected at 8 hours for measurement of cytokine concentrations by ELISA (A). Cells were collected at 4 hours for quantification of cytokine transcripts using real-time PCR (expressed as fold increases versus untreated parental cells) (B). (C) Representative flow cytometry plots (left) showing phagocytosis of FITC labeled heat killed E. coli (HKEC) and bar graph representing % of fluorescent cells and the MFI values (right). Ctrl, control. Ns, not significant; *, P <0.05; **, P <0.01; ***, P<0.005; ****, P<0.001. Each point represents a biological replicate. In all experiments, 4 to 5 different Ndufs4−/− clones were used. Data are shown as the mean ± SEM., Peer reviewed




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