Dataset.

Epigenetic heterogeneity among modules of individual Lavandula latifolia plants

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/227776
Digital.CSIC. Repositorio Institucional del CSIC
  • Herrera, Carlos M.
  • Bazaga, Pilar
  • Pérez, Ricardo
  • Alonso, Conchita
The dataset consists of two comma-delimited plain text files for global methylation and MS-AFLP data, respectively. In each file, each row refers to an individual module. For MS-AFLP columns refers to a specific (although anonymous) marker, identified by primer combination and size (base pairs). Three L. latifolia shrubs were sampled at a large population growing near Arroyo Aguaderillos in the Sierra de Cazorla (Jaén province, southeastern Spain; geographical coordinates 37.96157 N, 2.88389 W). Fresh leaf samples were collected from as many individual modules as possible in each plant. DNA cytosine methylation was determined for each sample by reversed phase HPLC with spectrofluorimetric detection. Global cytosine methylation was estimated as 100 x 5mdC/(5mdC + dC), where 5mdC and dC are the integrated areas under the peaks for 5-methyl-2'-deoxycytidine and 2'-deoxycytidine, respectively. The MS-AFLP analyses were performed using standard protocols involving the use of fluorescent dye-labeled selective primers. Each sample was fingerprinted using eight primer combinations, each with two (HpaII) or three (MseI) selective nucleotides. Fragment separation and detection was made using an ABI PRISM 3130xl DNA sequencer, only fragments ≥ 150 base pairs in size were considered and the presence (1) or absence (0) of fragments in each sample was scored manually by visualizing electropherograms with GeneMapper 3.7 software, This dataset includes: (i) global DNA cytosine methylation estimates (= percentage of all genomic cytosines that are methylated), obtained by reverse phase HPLC with spectrofluorimetric detection; and (ii) the scoring (presence/absence) of individual methylation-sensitive AFLP markers (MS-AFLP), for different modules of three adult, wild-grown, field-sampled plants of the shrub Lavandula latifolia., Peer reviewed
 
DOI: http://hdl.handle.net/10261/227776
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/227776

HANDLE: http://hdl.handle.net/10261/227776
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/227776
 
Ver en: http://hdl.handle.net/10261/227776
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/227776

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/227776
Dataset. 2021

EPIGENETIC HETEROGENEITY AMONG MODULES OF INDIVIDUAL LAVANDULA LATIFOLIA PLANTS

Digital.CSIC. Repositorio Institucional del CSIC
  • Herrera, Carlos M.
  • Bazaga, Pilar
  • Pérez, Ricardo
  • Alonso, Conchita
The dataset consists of two comma-delimited plain text files for global methylation and MS-AFLP data, respectively. In each file, each row refers to an individual module. For MS-AFLP columns refers to a specific (although anonymous) marker, identified by primer combination and size (base pairs). Three L. latifolia shrubs were sampled at a large population growing near Arroyo Aguaderillos in the Sierra de Cazorla (Jaén province, southeastern Spain; geographical coordinates 37.96157 N, 2.88389 W). Fresh leaf samples were collected from as many individual modules as possible in each plant. DNA cytosine methylation was determined for each sample by reversed phase HPLC with spectrofluorimetric detection. Global cytosine methylation was estimated as 100 x 5mdC/(5mdC + dC), where 5mdC and dC are the integrated areas under the peaks for 5-methyl-2'-deoxycytidine and 2'-deoxycytidine, respectively. The MS-AFLP analyses were performed using standard protocols involving the use of fluorescent dye-labeled selective primers. Each sample was fingerprinted using eight primer combinations, each with two (HpaII) or three (MseI) selective nucleotides. Fragment separation and detection was made using an ABI PRISM 3130xl DNA sequencer, only fragments ≥ 150 base pairs in size were considered and the presence (1) or absence (0) of fragments in each sample was scored manually by visualizing electropherograms with GeneMapper 3.7 software, This dataset includes: (i) global DNA cytosine methylation estimates (= percentage of all genomic cytosines that are methylated), obtained by reverse phase HPLC with spectrofluorimetric detection; and (ii) the scoring (presence/absence) of individual methylation-sensitive AFLP markers (MS-AFLP), for different modules of three adult, wild-grown, field-sampled plants of the shrub Lavandula latifolia., Peer reviewed




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