Dataset.
Primer sequences [Dataset]
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352126
Digital.CSIC. Repositorio Institucional del CSIC
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
To meet the physiological demands of the body, organs need to establish a functional tissue architecture and adequate size as the embryo develops to adulthood. In the liver, uni- and bipotent progenitor differentiation into hepatocytes and biliary epithelial cells (BECs), and their relative proportions, comprise the functional architecture. Yet, the contribution of individual liver progenitors at the organ level to both fates, and their specific proportion, is unresolved. Combining mathematical modelling with organ-wide, multispectral FRaeppli-NLS lineage tracing in zebrafish, we demonstrate that a precise BEC-to-hepatocyte ratio is established (i) fast, (ii) solely by heterogeneous lineage decisions from uni- and bipotent progenitors, and (iii) independent of subsequent cell type–specific proliferation. Extending lineage tracing to adulthood determined that embryonic cells undergo spatially heterogeneous three-dimensional growth associated with distinct environments. Strikingly, giant clusters comprising almost half a ventral lobe suggest lobe-specific dominant-like growth behaviours. We show substantial hepatocyte polyploidy in juveniles representing another hallmark of postembryonic liver growth. Our findings uncover heterogeneous progenitor contributions to tissue architecture-defining cell type proportions and postembryonic organ growth as key mechanisms forming the adult liver., Peer reviewed
DOI: http://hdl.handle.net/10261/352126
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352126
HANDLE: http://hdl.handle.net/10261/352126
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352126
Ver en: http://hdl.handle.net/10261/352126
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352126
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Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352126
Dataset. 2023
PRIMER SEQUENCES [DATASET]
Digital.CSIC. Repositorio Institucional del CSIC
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
To meet the physiological demands of the body, organs need to establish a functional tissue architecture and adequate size as the embryo develops to adulthood. In the liver, uni- and bipotent progenitor differentiation into hepatocytes and biliary epithelial cells (BECs), and their relative proportions, comprise the functional architecture. Yet, the contribution of individual liver progenitors at the organ level to both fates, and their specific proportion, is unresolved. Combining mathematical modelling with organ-wide, multispectral FRaeppli-NLS lineage tracing in zebrafish, we demonstrate that a precise BEC-to-hepatocyte ratio is established (i) fast, (ii) solely by heterogeneous lineage decisions from uni- and bipotent progenitors, and (iii) independent of subsequent cell type–specific proliferation. Extending lineage tracing to adulthood determined that embryonic cells undergo spatially heterogeneous three-dimensional growth associated with distinct environments. Strikingly, giant clusters comprising almost half a ventral lobe suggest lobe-specific dominant-like growth behaviours. We show substantial hepatocyte polyploidy in juveniles representing another hallmark of postembryonic liver growth. Our findings uncover heterogeneous progenitor contributions to tissue architecture-defining cell type proportions and postembryonic organ growth as key mechanisms forming the adult liver., Peer reviewed
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