Dataset.

EDEM-1 and EDEM-2 are required for CPL-1* degradation

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751
Digital.CSIC. Repositorio Institucional del CSIC
  • Ghenea, Simona
  • Chiritoiu, Marioara
  • Tacutu, Robi
  • Miranda-Vizuete, Antonio
  • Petrescu, Stefana Maria
S2 Fig. EDEM-1 and EDEM-2 are required for CPL-1* degradation (A) Fluorescence intensities of WT animals carrying the CPL-1* transgene treated with indicated concentrations of kifunensine. Values in scatter gram represent mean fluorescence/µm2 x 1000 in arbitrary units (AU). The red bars indicate the average ±SEM. ****P<0.0001; one way ANOVA with Dunnett post test. (B) Immunoblot analysis of CPL-1* degradation in edem-1 and edem-2 mutants carrying the rescuing EDEM-1::mCherry and EDEM-2::mCherry transgenes, respectively. Total protein lysates derived from edem-1 and edem-2 mutants carrying the CPL-1* and rescuing transgenes were separated by SDS-PAGE and immunoblotted with anti-GFP polyclonal antisera. Tubulin was used as loading control. (C) Histogram showing the densitometry values of the bands presented in (B) normalized to the value of WT condition (n=3 ± SEM, one way ANOVA with Fisher test), *P<0.05; **P<0.01)., Peer reviewed
 
DOI: http://hdl.handle.net/10261/331751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751

HANDLE: http://hdl.handle.net/10261/331751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751
 
Ver en: http://hdl.handle.net/10261/331751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751
Dataset. 2022

EDEM-1 AND EDEM-2 ARE REQUIRED FOR CPL-1* DEGRADATION

Digital.CSIC. Repositorio Institucional del CSIC
  • Ghenea, Simona
  • Chiritoiu, Marioara
  • Tacutu, Robi
  • Miranda-Vizuete, Antonio
  • Petrescu, Stefana Maria
S2 Fig. EDEM-1 and EDEM-2 are required for CPL-1* degradation (A) Fluorescence intensities of WT animals carrying the CPL-1* transgene treated with indicated concentrations of kifunensine. Values in scatter gram represent mean fluorescence/µm2 x 1000 in arbitrary units (AU). The red bars indicate the average ±SEM. ****P<0.0001; one way ANOVA with Dunnett post test. (B) Immunoblot analysis of CPL-1* degradation in edem-1 and edem-2 mutants carrying the rescuing EDEM-1::mCherry and EDEM-2::mCherry transgenes, respectively. Total protein lysates derived from edem-1 and edem-2 mutants carrying the CPL-1* and rescuing transgenes were separated by SDS-PAGE and immunoblotted with anti-GFP polyclonal antisera. Tubulin was used as loading control. (C) Histogram showing the densitometry values of the bands presented in (B) normalized to the value of WT condition (n=3 ± SEM, one way ANOVA with Fisher test), *P<0.05; **P<0.01)., Peer reviewed




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