Digital.CSIC. Repositorio Institucional del CSIC

oai:digital.csic.es:10261/339355

The following Supporting Information is available for this article: Table S1. Description of the target shrub species at the Aranjuez (Helianthemum squamatum) and Sorbas (Helianthemum squamatum, Helianthemum syriacum and Gypsophila struthium) field sites. Table S2. Results of the linear mixed model analyses of leaf gas exchange parameters measured in Helianthemum squamatum shrubs in Aranjuez and Helianthemum squamatum, Helianthemum syriacum and Gypsophila struthium shrubs in Sorbas in Spring in three consecutive growing seasons (2015-2017). Table S3. Mean (± SE) values for leaf nutrient concentrations (N, P and K in mg g-1), leaf mass area (LMA, mg cm-2), leaf area (LA, cm2), leaf thickness (LT, mm) and leaf isotopic C composition (13C) in Aranjuez (Helianthemum squamatum) and Sorbas (Helianthemum squamatum, Helianthemum syriacum and Gypsophila struthium) and across species and sites (Grand mean) in spring for three consecutive growing seasons (2015-2017). Table S4. Results of the linear mixed model analyses of for leaf nutrient concentrations (N, P and K), and contents (N, P and K content), leaf mass area (LMA), leaf thickness (LT) and leaf isotopic C composition (13C) measured in Helianthemum squamatum shrubs in Aranjuez and Helianthemum squamatum, Helianthemum syriacum and Gypsophila struthium shrubs in Sorbas in Spring in three consecutive growing seasons (2015-2017). Figure S1. Experimental plot of the combined warming and rainfall reduction treatment (W+RR). Figure S2 Overview of the experimental plots in the Aranjuez and Sorbas experimental stations and layout of the experimental plots. Figure S3. A priori conceptual structural equation model (SEM) depicting pathways by which climate change (W: warming and RR: rainfall reduction) influences plant photosynthetic nutrient use efficiency (PNutUE) through effects on the plant’s stomatal conductance (gs), photosynthetic rates (A), leaf morphology (leaf thickness: LT and leaf area: LA) and three leaf nutrients involved in photosynthesis and stomatal conductance regulation (Leaf Nut: N, P and K). Figure S4. Leaf gas exchange (Photosynthetic rate, stomatal conductance: gs, transpiration, ci/ca ratio and intrinsic and instantaneous water use efficiency) in Helianthemum squamatum in Aranjuez (H. sq A) and Helianthemum squamatum (H. sq S), Helianthemum syriacum (H. syr S) and Gypsophila struthium (G. st S) in Sorbas measured in spring. Figure S5 Relationships between net photosynthetic rates in a mass basis (Amass) and a) leaf N, b) leaf P and c) leaf K concentrations and net photosynthetic rates in an area basis (A) and d) leaf N, e) leaf P and f) leaf K concentrations. Figure S6. Relationships between the integrated water use efficiency (foliar 13C) and the photosynthetic nutrient use efficiency of a) leaf N (PNUE), b) leaf P (PPUE) and c) leaf K (PKUE). Figure S7. Standardized total (upper panel), direct (middle panel) and indirect (lower panel) effects derived from the structural equation modeling, including the effects of rainfall reduction (RR), warming (W), leaf nutrients (N, P or K, respectively), stomatal conductance (gs) and photosynthetic rates (A) on the photosynthetic use of leaf N (PNUE, black bars), leaf P (PPUE, light grey bars) and leaf K (PKUE, dark grey bars)., Peer reviewed