Dataset.
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2 [Dataset]
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371161
Digital.CSIC. Repositorio Institucional del CSIC
- Mertinkus, Klara R.
- Oxenfarth, Andreas
- Richter, Christian
- Wacker, Anna
- Mata, Carlos P.
- Carazo, Jose M.
- Schlundt, Andreas
- Schwalbe, Harald
Throughout the family of coronaviruses, structured RNA elements within the 5′ region of the genome are highly conserved. The fifth stem-loop element from SARS-CoV-2 (5_SL5) represents an example of an RNA structural element, repeatedly occurring in coronaviruses. It contains a conserved, repetitive fold within its substructures SL5a and SL5b. We herein report the detailed characterization of the structure and dynamics of elements SL5a and SL5b that are located immediately upstream of the SARS-CoV-2 ORF1a/b start codon. Exploiting the unique ability of solution NMR methods, we show that the structures of both apical loops are modulated by structural differences in the remote parts located in their stem regions. We further integrated our high-resolution models of SL5a/b into the context of full-length 5_SL5 structures by combining different structural biology methods. Finally, we evaluated the impact of the two most common VoC mutations within 5_SL5 with respect to individual base-pair stability., Peer reviewed
DOI: http://hdl.handle.net/10261/371161, https://digital.csic.es/handle/10261/371150
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371161
HANDLE: http://hdl.handle.net/10261/371161, https://digital.csic.es/handle/10261/371150
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371161
Ver en: http://hdl.handle.net/10261/371161, https://digital.csic.es/handle/10261/371150
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371161
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1 Documentos relacionados
1 Documentos relacionados
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371150
Artículo científico (article). 2024
DISSECTING THE CONFORMATIONAL HETEROGENEITY OF STEM-LOOP SUBSTRUCTURES OF THE FIFTH ELEMENT IN THE 5'-UNTRANSLATED REGION OF SARS-COV-2
Digital.CSIC. Repositorio Institucional del CSIC
- Mertinkus, Klara R.
- Oxenfarth, Andreas
- Richter, Christian
- Wacker, Anna
- Mata, Carlos P.
- Carazo, José M.
- Schlundt, Andreas
- Schwalbe, Harald
Throughout the family of coronaviruses, structured RNA elements within the 5' region of the genome are highly conserved. The fifth stem-loop element from SARS-CoV-2 (5_SL5) represents an example of an RNA structural element, repeatedly occurring in coronaviruses. It contains a conserved, repetitive fold within its substructures SL5a and SL5b. We herein report the detailed characterization of the structure and dynamics of elements SL5a and SL5b that are located immediately upstream of the SARS-CoV-2 ORF1a/b start codon. Exploiting the unique ability of solution NMR methods, we show that the structures of both apical loops are modulated by structural differences in the remote parts located in their stem regions. We further integrated our high-resolution models of SL5a/b into the context of full-length 5_SL5 structures by combining different structural biology methods. Finally, we evaluated the impact of the two most common VoC mutations within 5_SL5 with respect to individual base-pair stability., This work was supported by state of Hesse (BMRZ). Funding for the work comes from the IWB-EFRE-program 20007375 of the State of Hesse, the Deutsche Forschungsgemeinschaft (DFG) through CRC 902: “Molecular Principles of RNA-based regulation” (Project number 161793742) and through individual grant (project number 495006306 H.S.). SAXS measurements were facilitated by the BAGs with numbers SAXS990, 1102, and 1106 funded by DESY/EMBL Hamburg. The authors JCP and JMC acknowledge the financial support from the Ministry of Science, Innovation and Universities (BDNS n. 716450) to the Instruct Image Processing Center (I2PC) as part of the Spanish participation in Instruct-ERIC, the European strategic infrastructure project (ESFRI) in the area of structural biology. AS was funded by DFG through grant numbers SCHL2062/2-1 and 2-2 as well as by the Johanna Quandt Young Academy at Goethe University (grant number 2019/AS01)., Peer reviewed
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1 Versiones
1 Versiones
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371161
Dataset. 2024
DISSECTING THE CONFORMATIONAL HETEROGENEITY OF STEM-LOOP SUBSTRUCTURES OF THE FIFTH ELEMENT IN THE 5'-UNTRANSLATED REGION OF SARS-COV-2 [DATASET]
Digital.CSIC. Repositorio Institucional del CSIC
- Mertinkus, Klara R.
- Oxenfarth, Andreas
- Richter, Christian
- Wacker, Anna
- Mata, Carlos P.
- Carazo, Jose M.
- Schlundt, Andreas
- Schwalbe, Harald
Throughout the family of coronaviruses, structured RNA elements within the 5′ region of the genome are highly conserved. The fifth stem-loop element from SARS-CoV-2 (5_SL5) represents an example of an RNA structural element, repeatedly occurring in coronaviruses. It contains a conserved, repetitive fold within its substructures SL5a and SL5b. We herein report the detailed characterization of the structure and dynamics of elements SL5a and SL5b that are located immediately upstream of the SARS-CoV-2 ORF1a/b start codon. Exploiting the unique ability of solution NMR methods, we show that the structures of both apical loops are modulated by structural differences in the remote parts located in their stem regions. We further integrated our high-resolution models of SL5a/b into the context of full-length 5_SL5 structures by combining different structural biology methods. Finally, we evaluated the impact of the two most common VoC mutations within 5_SL5 with respect to individual base-pair stability., Peer reviewed
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1106