Digital.CSIC. Repositorio Institucional del CSIC

oai:digital.csic.es:10261/339979

Figure S1: predicted secondary structure of circRAJ31. Figure S2: analysis of the secondary structure of the 5 UTR and the first 30 bp of the coding sequence of gfp or cat. Figure S3: electrophoretic assay and sequencing results of RT-PCR after in vitro transcription. Figure S4: putative sequence of the RT-PCR misprocessing reaction. Figure S5: response of the system to varying concentrations of aTc (which controls expression of the riboregulator). Figure S6: characterization of mutant systems. Figure S7: growth of cells cotransformed with plasmids expressing circRAJ31 and CamR on plates without chloramphenicol (Cam) and on plates with chloramphenicol and different inducers. Figure S8: growth curves with a cis-repressed gene coding for chloramphenicol acetyltransferase (CamR). Figure S9: additional growth curves for different concentrations of Cam. Table S2: sequences of the primers used in this work., Peer reviewed