Dataset.

Arrhythmic Effects Evaluated on Caenorhabditis elegans: The Case of Polypyrrole Nanoparticles [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360401
Digital.CSIC. Repositorio Institucional del CSIC
  • Srinivasan, Sumithra Y.
  • Alvarez-Illera, Pilar
  • Kukhtar, Dmytro
  • Benseny-Cases, Núria
  • Cerón, Julián
  • Álvarez. Javier
  • Fonteriz, Rosalba I.
  • Montero, Mayte
  • Laromaine, Anna
Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes’ pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs’ biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs., Peer reviewed
 
DOI: http://hdl.handle.net/10261/360401
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360401

HANDLE: http://hdl.handle.net/10261/360401
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360401
 
Ver en: http://hdl.handle.net/10261/360401
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360401

UVaDOC. Repositorio Documental de la Universidad de Valladolid
oai:uvadoc.uva.es:10324/66078
Artículo científico (article). 2023

ARRHYTHMIC EFFECTS EVALUATED ON CAENORHABDITIS ELEGANS: THE CASE OF POLYPYRROLE NANOPARTICLES

UVaDOC. Repositorio Documental de la Universidad de Valladolid
  • Srinivasan, Sumithra Yasaswini
  • Álvarez Illera, María Pilar
  • Kukhtar, Dmytro
  • Benseny-Cases, Núria
  • Cerón, Julián
  • Álvarez Martín, Javier
  • Fonteriz García, Rosalba Inés
  • Montero Zoccola, María Teresa
  • Laromaine, Anna
Producción Científica, Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes’ pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs’ biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs., MICINN project PID2021-122239OB-I00, BEAT project (FUNFUTURE-FIP- 2020), European Union NextGEM project 101057527., European Union’s Horizon 2020 - Marie Sklodowska-Curie grant agreement No 754397





Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/342017
Artículo científico (article). 2024

ARRHYTHMIC EFFECTS EVALUATED ON CAENORHABDITIS ELEGANS: THE CASE OF POLYPYRROLE NANOPARTICLES

Digital.CSIC. Repositorio Institucional del CSIC
  • Srinivasan, Sumithra Y.
  • Alvarez-Illera, Pilar
  • Kukhtar, Dmytro
  • Benseny-Cases, Núria
  • Cerón, Julián
  • Álvarez. Javier
  • Fonteriz, Rosalba I.
  • Montero, Mayte
  • Laromaine, Anna
Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes’ pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs’ biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs., This work was supported by the Ministry of Science and Innovation (MICINN) “Severo Ochoa” program for centers of excellence through the BEAT project (FUNFUTURE-FIP-2020), MICINN project PID2021-122239OB-I00 and partly supported by the European Union’s Horizon Europe research and innovation program under grant agreement No 101057527 (NextGEM). Funded by the European Union. Some experiments were performed at MIRAS beamline at ALBA Synchrotron with the collaboration of ALBA staff. SYS is enrolled in the Materials Science Ph.D. program of Universitat Autonoma de Barcelona (UAB). SYS acknowledges financial support from DOC-FAM, European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 754397. AL and SYS participate in the networks of EPNOE, Red Nanocare2.0 , and the CSIC Interdisciplinary Platform for Sustainable Plastics toward a Circular Economy, SUSPLAST. The authors acknowledge the use of Biorender.com, Peer reviewed




Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/214026
Artículo científico (article). 2023

ARRHYTHMIC EFFECTS EVALUATED ON CAENORHABDITIS ELEGANS: THE CASE OF POLYPYRROLE NANOPARTICLES

Dipòsit Digital de la UB
  • Srinivasan, Sumithra Yasaswini
  • Álvarez Illera, Pilar
  • Kukhtar, Dmytro
  • Benseny Cases, Núria
  • Cerón, Julián
  • Álvarez, Javier
  • Fonteriz, Rosalba I.
  • Montero, Mayte
  • Laromaine, Anna
Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes' pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs' biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs.




Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/360401
Dataset. 2023

ARRHYTHMIC EFFECTS EVALUATED ON CAENORHABDITIS ELEGANS: THE CASE OF POLYPYRROLE NANOPARTICLES [DATASET]

Digital.CSIC. Repositorio Institucional del CSIC
  • Srinivasan, Sumithra Y.
  • Alvarez-Illera, Pilar
  • Kukhtar, Dmytro
  • Benseny-Cases, Núria
  • Cerón, Julián
  • Álvarez. Javier
  • Fonteriz, Rosalba I.
  • Montero, Mayte
  • Laromaine, Anna
Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes’ pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs’ biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs., Peer reviewed




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