Dataset.

Analysis of the importance of each serine or threonine followed by proline in Cbk1-6E

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361053
Digital.CSIC. Repositorio Institucional del CSIC
  • Foltman, Magdalena
  • Méndez, Iván
  • Bech-Serra, Joan J.
  • de la Torre, Carolina
  • Brace, Jennifer L.
  • Weiss, Eric L.
  • Lucas, María
  • Queralt, Ethel
  • Sánchez-Díaz, Alberto
(A) cbk1-5E-E164S cbk1-aid cdc15-2 (YMF3905), (B) cbk1-5E-E251S cbk1-aid cdc15-2 (YMF3910), (C) cbk1-5E-E409S cbk1-aid cdc15-2 (YMF3995), (D) cbk1-5E-E615T cbk1-aid cdc15-2 (YMF3906), and (E) cbk1-5E-E711S cbk1-aid cdc15-2 (YMF3907) cells were grown in YPD and arrested in late anaphase by shifting the temperature to 37 °C before the addition of rapamycin to half of the culture for 20 min. To allow progression through the cell cycle, cells were released in the absence (i) or presence (ii) of rapamycin. Samples were taken at the indicated times to determine cell-cycle progression by flow cytometry. (F) 3D Cbk1 structure (PDB 4LQS [48]) in which different protein domains are highlighted. Residue T574 in the activation loop, together with residues D475 and S409 in the kinase domain are denoted. (G) cbk1-T574A cbk1-aid cdc15-2 cells (YMF4191) were grown as above. FACS graphs can be found in the supplementary FACS file (S1 File)., pbio.3002263.s005.pdf, Peer reviewed
 
DOI: http://hdl.handle.net/10261/361053
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361053

HANDLE: http://hdl.handle.net/10261/361053
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361053
 
Ver en: http://hdl.handle.net/10261/361053
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361053

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/361053
Dataset. 2023

ANALYSIS OF THE IMPORTANCE OF EACH SERINE OR THREONINE FOLLOWED BY PROLINE IN CBK1-6E

Digital.CSIC. Repositorio Institucional del CSIC
  • Foltman, Magdalena
  • Méndez, Iván
  • Bech-Serra, Joan J.
  • de la Torre, Carolina
  • Brace, Jennifer L.
  • Weiss, Eric L.
  • Lucas, María
  • Queralt, Ethel
  • Sánchez-Díaz, Alberto
(A) cbk1-5E-E164S cbk1-aid cdc15-2 (YMF3905), (B) cbk1-5E-E251S cbk1-aid cdc15-2 (YMF3910), (C) cbk1-5E-E409S cbk1-aid cdc15-2 (YMF3995), (D) cbk1-5E-E615T cbk1-aid cdc15-2 (YMF3906), and (E) cbk1-5E-E711S cbk1-aid cdc15-2 (YMF3907) cells were grown in YPD and arrested in late anaphase by shifting the temperature to 37 °C before the addition of rapamycin to half of the culture for 20 min. To allow progression through the cell cycle, cells were released in the absence (i) or presence (ii) of rapamycin. Samples were taken at the indicated times to determine cell-cycle progression by flow cytometry. (F) 3D Cbk1 structure (PDB 4LQS [48]) in which different protein domains are highlighted. Residue T574 in the activation loop, together with residues D475 and S409 in the kinase domain are denoted. (G) cbk1-T574A cbk1-aid cdc15-2 cells (YMF4191) were grown as above. FACS graphs can be found in the supplementary FACS file (S1 File)., pbio.3002263.s005.pdf, Peer reviewed




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