Dataset.

Identifying natural bioactive peptides from the common octopus (Octopus vulgaris Cuvier, 1797) skin mucus by-products using proteogenomic analysis [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/355058
Digital.CSIC. Repositorio Institucional del CSIC
  • Gestal, C.
  • Carrera, Mónica
22 files, Data for the article Identifying Natural Bioactive Peptides from the Common Octopus (Octopus vulgaris Cuvier, 1797) Skin Mucus By-Products Using Proteogenomic Analysis, https://doi.org/10.3390/ijms24087145, No
 
DOI: http://hdl.handle.net/10261/355058
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/355058

HANDLE: http://hdl.handle.net/10261/355058
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/355058
 
Ver en: http://hdl.handle.net/10261/355058
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/355058

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/283675
. 2022

IDENTIFYING NATURAL BIOACTIVE PEPTIDES FROM THE COMMON OCTOPUS (OCTOPUS VULGARIS) INK USING PROTEOMIC ANALYSIS

Digital.CSIC. Repositorio Institucional del CSIC
  • Imran, Md Abdus Shukur
  • Carrera, Mónica
  • Pérez-Polo, Sara
  • Pérez Vázquez, Jaime
  • Barros, Lorena
  • Dios, S.
  • Gestal, C.
Poster.-- 2nd International Symposium Mucosal Health in Aquaculture, Madrid, 3-6 October 2022, The common octopus (Octopus vulgaris) is nowadays the most demanded cephalopod species for human consumption. This species was also postulated for aquaculture diversification to supply its increasing demand in the market worldwide, which only relies on continuously declined field captures. In addition, they serve as model species for biomedical and behavioral studies. Body parts of marine species are usually removed before reaching the final consumer as by-products in order to improve preservation, reduce shipping weight and increase product quality. These by-products have recently attracted great attention due to discovery of the presence of several relevant bioactive compounds. Particularly, the common octopus ink has been described as anti-microbial and anti-oxidant properties, among others. We applied the emerging science of Proteomics-based Systems Biology to generate a common octopus reference proteome for the screening of potential bioactive peptides from fishing discards and by-products such as ink. Octopus were captured in NW Atlantic waters (Galicia, Spain), by local fishermen and transported in ice to the laboratory. Sac inks were dissected and processed for ink protein extraction/purification and freeze-dried. A shotgun proteomics approach combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) using an Orbitrap Elite instrument was then used to create a reference dataset from octopus ink. Protein-based bioinformatics analyses were then carried out to predict and characterize potential bioactive peptides. Octopus ink proteome was created by merging 2693 identified spectra (PSMs) from 1432 different peptides. Finally, a total 361 non-redundant annotated proteins were identified from these peptides. The results obtained show the potential of bioactive compounds from octopus body parts that are usually discarded. These bioactive compounds can exert health beneficial properties and are therefore considered as lead compounds for the development of pharmacological, functional foods or nutraceuticals, No




Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/308099
Artículo científico (article). 2023

IDENTIFYING NATURAL BIOACTIVE PEPTIDES FROM THE COMMON OCTOPUS (OCTOPUS VULGARIS CUVIER, 1797) SKIN MUCUS BY-PRODUCTS USING PROTEOGENOMIC ANALYSIS

Digital.CSIC. Repositorio Institucional del CSIC
  • Pérez-Polo, Sara
  • Imran, Md Abdus Shukur
  • Dios, S.
  • Pérez Vázquez, Jaime
  • Barros, Lorena
  • Carrera, Mónica
  • Gestal, C.
19 pages, 6 figures, 3 tables.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, The common octopus is a cephalopod species subject to active fisheries, with great potential in the aquaculture and food industry, and which serves as a model species for biomedical and behavioral studies. The analysis of the skin mucus allows us to study their health in a non-invasive way, by using a hardly exploited discard of octopus in the fishing sector. A shotgun proteomics approach combined with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) using an Orbitrap-Elite instrument was used to create a reference dataset from octopus skin mucus. The final proteome compilation was investigated by integrated in-silico studies, including Gene Ontology (GO), the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, network studies, and prediction and characterization analysis of potential bioactive peptides. This work presents the first proteomic analysis of the common octopus skin mucus proteome. This library was created by merging 5937 identified spectra of 2038 different peptides. A total of 510 non-redundant proteins were identified. Obtained results show proteins closely related to the defense, which highlight the role of skin mucus as the first barrier of defense and the interaction with the environment. Finally, the potential of the bioactive peptides with antimicrobial properties, and their possible application in biomedicine, pharmaceutical, and nutraceutical industry was addressed., This research was supported by Projects PID2019-103845RB-C21 and PID2020-119906GB-I00 funded by MCIN/AEI/10.13039/501100011033, and the Project AGL2017-89475-C2-1-R funded by MCIN/AEI/10.13039/501100011033, and for FEDER Una manera de hacer Europa, and the JAE Intro research grant “JAE Intro ICU”, within the framework of the “Junta para la Ampliación de Estudios” 2021 program., Peer reviewed




Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/344392
. 2023

IDENTIFYING NATURAL BIOACTIVE PEPTIDES FROM THE COMMON OCTOPUS (OCTOPUS VULGARIS) SKIN MUCUS BY-PRODUCTS USING PROTEOMIC ANALYSIS

Digital.CSIC. Repositorio Institucional del CSIC
  • Pérez-Polo, Sara
  • Imran, Md Abdus Shukur
  • Dios, S.
  • Pérez Vázquez, Jaime
  • Barros, Lorena
  • Carrera, Mónica
  • Gestal, C.
21st International Conference on Diseases of Fish and Shellfish, 11-14 September 2023, Aberdeen, UK, The common octopus (Octopus vulgaris) is one of the world’s economically important species, subject of active fisheries and highly appreciated as food. In addition, they have a great potential as aquaculture species, and serve as model species for biomedical and behavioral studies. Bioactive compounds from marine organisms’ by-products can exert health beneficial properties and are considered as lead compounds for the development of pharmacological, functional foods or nutraceuticals. Particularly, the common octopus skin mucus has been described as anti-microbial and anti-oxidant properties, among others. We applied the emerging science of Proteomics-based Systems Biology to generate a common octopus reference proteome for the screening of potential bioactive peptides from fishing discards and by-products such as skin mucus. Octopus were captured in NW Atlantic waters (Galicia, Spain), by local fishermen and transported in ice to the laboratory where they were processed for mucus protein extraction/purification and freeze-dried. A shotgun proteomics approach combined with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) using an Orbitrap Elite instrument was then used to create a reference dataset from octopus skin mucus. Protein-based bioinformatics analyses were then carried out to predict and characterize potential bioactive peptides. The first proteomics analysis for Octopus vulgaris mucus skin proteome is presented in this work. This library was created merging a total of 5937 identified spectra (PSMs) from 2038 different peptides. Finally, a total of 510 non-redundant annotated proteins were identified. The results obtained show the potential of bioactive compounds from octopus body parts that are usually discarded and therefore highlight their application in biomedicine and in the pharmaceutical and nutraceutical industry, No




Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/355058
Dataset. 2022

IDENTIFYING NATURAL BIOACTIVE PEPTIDES FROM THE COMMON OCTOPUS (OCTOPUS VULGARIS CUVIER, 1797) SKIN MUCUS BY-PRODUCTS USING PROTEOGENOMIC ANALYSIS [DATASET]

Digital.CSIC. Repositorio Institucional del CSIC
  • Gestal, C.
  • Carrera, Mónica
22 files, Data for the article Identifying Natural Bioactive Peptides from the Common Octopus (Octopus vulgaris Cuvier, 1797) Skin Mucus By-Products Using Proteogenomic Analysis, https://doi.org/10.3390/ijms24087145, No




Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/355492
Dataset. 2023

IDENTIFYING NATURAL BIOACTIVE PEPTIDES FROM THE INK BY-PRODUCT OF COMMON OCTOPUS (OCTOPUS VULGARIS) USING PROTEOMICS

Digital.CSIC. Repositorio Institucional del CSIC
  • Gestal, C.
  • Carrera, Mónica
  • Dios, S.
10 files, f.MSV000089896/ccms_parameters/params.xml; f.MSV000089896/ccms_result/MCMMCM34021201_02_03_INK_BATCH_FDR_UNIGENE_PULPO_plus_Cephalopoda_Percolator.mzTab; f.MSV000089896/ccms_statistics/statistics.tsv; f.MSV000089896/peak/MCMMCM34021201.mzML; f.MSV000089896/peak/MCMMCM34021202.mzML; f.MSV000089896/peak/MCMMCM34021203.mzML; f.MSV000089896/raw/MCMMCM34021201.raw; f.MSV000089896/raw/MCMMCM34021202.raw; f.MSV000089896/raw/MCMMCM34021203.raw; f.MSV000089896/result/MCMMCM34021201_02_03_INK_BATCH_FDR_UNIGENE_PULPO_plus_Cephalopoda_Percolator.mzTab, Peer reviewed




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