Dataset.
Data of Manuscript Potential of Dry-Cured Ham Bones as a Sustainable Source to Obtain Antioxidant and DPP-IV Inhibitory Extracts
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371528
Digital.CSIC. Repositorio Institucional del CSIC
- Carrera Alvarado, Gisela
- Toldrá Vilardell, Fidel
- Mora, Leticia
Excel file including: Degree of hydrolysis, antioxidant activity evaluated by DPPH, FRAP and ABTS assays, inhibition of DPP-IV of 3 enzymatic hydrolysates of Spanish dry-cured ham bone, peptide separation by reverse phase chromatography (RP-HPLC) and hydrophilic interaction liquid chromatography (HILIC); peptide identification by mass spectrometry in tandem and free amino acid content of each hydrolysate, The utilization of animal bones as a protein source could be used as a sustainable pathway for the production of bioactive compounds. In this study, bones were pretreated with pepsin enzyme (PEP) and then sequentially hydrolyzed with Alcalase (PA) and Alcalase, as well as Protana prime (PAPP). The degree of hydrolysis, antioxidant activity, and DPP-IV inhibitory activity were measured. All three hydrolysates showed antioxidant and DPP-IV inhibitory activity; however, the highest result in both bioactivities was obtained with the PAPP hydrolysate. The obtained free amino acid content was 54.62, 88.12, and 668.46 mg/100 mL of hydrolyzed in PEP, PA, and PAPP, respectively. Pepsin pretreatment did not significantly affect the degree of hydrolysis; however, it is suggested that it promoted the cleavage of certain bonds for subsequent protease action. Accordingly, a total of 550 peptides were identified in PEP hydrolysate, 1087 in PA hydrolysate, and 1124 in PAPP hydrolysate using an LC-MS/MS approach. Pepsin pretreatment could be an effective method in the utilization of bone sources for the production of antioxidant and hypoglycemic peptides., Grant PID2020-119684RB-I00 funded by MCIN/ AEI /10.13039/501100011033 is acknowledged. Grant GRISOLIAP/2020/021 de la Consellería de Innovació, Universitats, Ciència i Societat Digital de la Generalitat Valenciana (G.C.-A.) is also acknowledged. Grant CEX2021-001189-S funded by MCIN/ AEI /10.13039/501100011033 is acknowledged, With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S), Peer reviewed
DOI: http://hdl.handle.net/10261/371528
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371528
HANDLE: http://hdl.handle.net/10261/371528
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371528
Ver en: http://hdl.handle.net/10261/371528
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371528
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Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/371528
Dataset. 2024
DATA OF MANUSCRIPT POTENTIAL OF DRY-CURED HAM BONES AS A SUSTAINABLE SOURCE TO OBTAIN ANTIOXIDANT AND DPP-IV INHIBITORY EXTRACTS
Digital.CSIC. Repositorio Institucional del CSIC
- Carrera Alvarado, Gisela
- Toldrá Vilardell, Fidel
- Mora, Leticia
Excel file including: Degree of hydrolysis, antioxidant activity evaluated by DPPH, FRAP and ABTS assays, inhibition of DPP-IV of 3 enzymatic hydrolysates of Spanish dry-cured ham bone, peptide separation by reverse phase chromatography (RP-HPLC) and hydrophilic interaction liquid chromatography (HILIC); peptide identification by mass spectrometry in tandem and free amino acid content of each hydrolysate, The utilization of animal bones as a protein source could be used as a sustainable pathway for the production of bioactive compounds. In this study, bones were pretreated with pepsin enzyme (PEP) and then sequentially hydrolyzed with Alcalase (PA) and Alcalase, as well as Protana prime (PAPP). The degree of hydrolysis, antioxidant activity, and DPP-IV inhibitory activity were measured. All three hydrolysates showed antioxidant and DPP-IV inhibitory activity; however, the highest result in both bioactivities was obtained with the PAPP hydrolysate. The obtained free amino acid content was 54.62, 88.12, and 668.46 mg/100 mL of hydrolyzed in PEP, PA, and PAPP, respectively. Pepsin pretreatment did not significantly affect the degree of hydrolysis; however, it is suggested that it promoted the cleavage of certain bonds for subsequent protease action. Accordingly, a total of 550 peptides were identified in PEP hydrolysate, 1087 in PA hydrolysate, and 1124 in PAPP hydrolysate using an LC-MS/MS approach. Pepsin pretreatment could be an effective method in the utilization of bone sources for the production of antioxidant and hypoglycemic peptides., Grant PID2020-119684RB-I00 funded by MCIN/ AEI /10.13039/501100011033 is acknowledged. Grant GRISOLIAP/2020/021 de la Consellería de Innovació, Universitats, Ciència i Societat Digital de la Generalitat Valenciana (G.C.-A.) is also acknowledged. Grant CEX2021-001189-S funded by MCIN/ AEI /10.13039/501100011033 is acknowledged, With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S), Peer reviewed
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