Dataset.
Advanced Binary Guanosine and Guanosine 5'‑Monophosphate Cell-Laden Hydrogels for Soft Tissue Reconstruction by 3D Bioprinting [Dataset]
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/356211
Digital.CSIC. Repositorio Institucional del CSIC
- Godoy-Gallardo, María
- Merino-Gómez, Maria
- Mateos-Timoneda, Miguel A.
- Eckhard, Ulrich
- Gil, Francisco Javier
- Pérez, Román A.
Soft tissue defects or pathologies frequently necessitate the use of biomaterials that provide the volume required for subsequent vascularization and tissue formation as autrografts are not always a feasible alternative. Supramolecular hydrogels represent promising candidates because of their 3D structure, which resembles the native extracellular matrix, and their capacity to entrap and sustain living cells. Guanosine-based hydrogels have emerged as prime candidates in recent years since the nucleoside self-assembles into well-ordered structures like G-quadruplexes by coordinating K+ ions and π–π stacking, ultimately forming an extensive nanofibrillar network. However, such compositions were frequently inappropriate for 3D printing due to material spreading and low shape stability over time. Thus, the present work aimed to develop a binary cell-laden hydrogel capable of ensuring cell survival while providing enough stability to ensure scaffold biointegration during soft tissue reconstruction. For that purpose, a binary hydrogel made of guanosine and guanosine 5'-monophosphate was optimized, rat mesenchymal stem cells were entrapped, and the composition was bioprinted. To further increase stability, the printed structure was coated with hyperbranched polyethylenimine. Scanning electron microscopic studies demonstrated an extensive nanofibrillar network, indicating excellent G-quadruplex formation, and rheological analysis confirmed good printing and thixotropic qualities. Additionally, diffusion tests using fluorescein isothiocyanate labeled-dextran (70, 500, and 2000 kDa) showed that nutrients of various molecular weights may diffuse through the hydrogel scaffold. Finally, cells were evenly distributed throughout the printed scaffold, cell survival was 85% after 21 days, and lipid droplet formation was observed after 7 days under adipogenic conditions, indicating successful differentiation and proper cell functioning. To conclude, such hydrogels may enable the 3D bioprinting of customized scaffolds perfectly matching the respective soft tissue defect, thereby potentially improving the outcome of the tissue reconstruction intervention., Peer reviewed
DOI: http://hdl.handle.net/10261/356211
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/356211
HANDLE: http://hdl.handle.net/10261/356211
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/356211
Ver en: http://hdl.handle.net/10261/356211
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/356211
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1 Documentos relacionados
1 Documentos relacionados
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/341508
Artículo científico (article). 2024
ADVANCED BINARY GUANOSINE AND GUANOSINE 5'-MONOPHOSPHATE CELL-LADEN HYDROGELS FOR SOFT TISSUE RECONSTRUCTION BY 3D BIOPRINTING
Digital.CSIC. Repositorio Institucional del CSIC
- Godoy-Gallardo, María
- Merino-Gómez, Maria
- Mateos-Timoneda, Miguel A.
- Eckhard, Ulrich
- Gil, Francisco Javier
- Pérez, Román A.
Soft tissue defects or pathologies frequently necessitate the use of biomaterials that provide the volume required for subsequent vascularization and tissue formation as autrografts are not always a feasible alternative. Supramolecular hydrogels represent promising candidates because of their 3D structure, which resembles the native extracellular matrix, and their capacity to entrap and sustain living cells. Guanosine-based hydrogels have emerged as prime candidates in recent years since the nucleoside self-assembles into well-ordered structures like G-quadruplexes by coordinating K+ ions and π–π stacking, ultimately forming an extensive nanofibrillar network. However, such compositions were frequently inappropriate for 3D printing due to material spreading and low shape stability over time. Thus, the present work aimed to develop a binary cell-laden hydrogel capable of ensuring cell survival while providing enough stability to ensure scaffold biointegration during soft tissue reconstruction. For that purpose, a binary hydrogel made of guanosine and guanosine 5'-monophosphate was optimized, rat mesenchymal stem cells were entrapped, and the composition was bioprinted. To further increase stability, the printed structure was coated with hyperbranched polyethylenimine. Scanning electron microscopic studies demonstrated an extensive nanofibrillar network, indicating excellent G-quadruplex formation, and rheological analysis confirmed good printing and thixotropic qualities. Additionally, diffusion tests using fluorescein isothiocyanate labeled-dextran (70, 500, and 2000 kDa) showed that nutrients of various molecular weights may diffuse through the hydrogel scaffold. Finally, cells were evenly distributed throughout the printed scaffold, cell survival was 85% after 21 days, and lipid droplet formation was observed after 7 days under adipogenic conditions, indicating successful differentiation and proper cell functioning. To conclude, such hydrogels may enable the 3D bioprinting of customized scaffolds perfectly matching the respective soft tissue defect, thereby potentially improving the outcome of the tissue reconstruction intervention., M.G.G. has received funding from the postdoctoral fellowship programme Beatriu de Pinós (2018 BP 00155), funded by the Secretary of Universities and Research (Government of Catalonia) and by the Horizon 2020 programme of research and innovation of the European Union under the Marie Sklodowska-Curie grant agreement No 801370. M.A.M.T. is supported by the Spanish Ministry of Science with the project (RTI2018-096320 B-C21, 2018). U.E. received funding from the Beatriu de Pinós (2018 BP 00163) and Ramón y Cajal (RYC2020-029773-I) programs. R.A.P. is supported by the Spanish Ministry by the Ramón y Cajal Program (RYC2018-025977-I) and MINECO/FEDER project (RTI2018-096088-J-100). Additional financial support was provided by the Government of Catalonia (2017 SGR 708)., No
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1 Versiones
1 Versiones
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/356211
Dataset. 2023
ADVANCED BINARY GUANOSINE AND GUANOSINE 5'‑MONOPHOSPHATE CELL-LADEN HYDROGELS FOR SOFT TISSUE RECONSTRUCTION BY 3D BIOPRINTING [DATASET]
Digital.CSIC. Repositorio Institucional del CSIC
- Godoy-Gallardo, María
- Merino-Gómez, Maria
- Mateos-Timoneda, Miguel A.
- Eckhard, Ulrich
- Gil, Francisco Javier
- Pérez, Román A.
Soft tissue defects or pathologies frequently necessitate the use of biomaterials that provide the volume required for subsequent vascularization and tissue formation as autrografts are not always a feasible alternative. Supramolecular hydrogels represent promising candidates because of their 3D structure, which resembles the native extracellular matrix, and their capacity to entrap and sustain living cells. Guanosine-based hydrogels have emerged as prime candidates in recent years since the nucleoside self-assembles into well-ordered structures like G-quadruplexes by coordinating K+ ions and π–π stacking, ultimately forming an extensive nanofibrillar network. However, such compositions were frequently inappropriate for 3D printing due to material spreading and low shape stability over time. Thus, the present work aimed to develop a binary cell-laden hydrogel capable of ensuring cell survival while providing enough stability to ensure scaffold biointegration during soft tissue reconstruction. For that purpose, a binary hydrogel made of guanosine and guanosine 5'-monophosphate was optimized, rat mesenchymal stem cells were entrapped, and the composition was bioprinted. To further increase stability, the printed structure was coated with hyperbranched polyethylenimine. Scanning electron microscopic studies demonstrated an extensive nanofibrillar network, indicating excellent G-quadruplex formation, and rheological analysis confirmed good printing and thixotropic qualities. Additionally, diffusion tests using fluorescein isothiocyanate labeled-dextran (70, 500, and 2000 kDa) showed that nutrients of various molecular weights may diffuse through the hydrogel scaffold. Finally, cells were evenly distributed throughout the printed scaffold, cell survival was 85% after 21 days, and lipid droplet formation was observed after 7 days under adipogenic conditions, indicating successful differentiation and proper cell functioning. To conclude, such hydrogels may enable the 3D bioprinting of customized scaffolds perfectly matching the respective soft tissue defect, thereby potentially improving the outcome of the tissue reconstruction intervention., Peer reviewed
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