Data from: Analysis of the porcine APOA2 gene expression in liver, polymorphisms identification and association with fatty acid composition traits

Digital.CSIC. Repositorio Institucional del CSIC
  • Ballester, María
  • Revilla, Manuel
  • Puig-Oliveras, Anna
  • Marchesi, J.A.P.
  • Castelló, Anna
  • Corominas, Jordi
  • Fernández, Ana Isabel
  • Folch, Josep María
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Porcine APOA2 gene expression in liver
Data from Analysis of the porcine APOA2 gene expression in liver, polymorphisms identification and association with fatty acid composition traits. Contains SNP genotypes for GWAS and eGWAS studies, mRNA expression of APOA2 in liver, and fatty acid composition in backfat and muscle, APOA2 is a protein implicated in triglyceride, fatty acid and glucose metabolism. In pigs, the APOA2 gene is located on pig chromosome 4 (SSC4) in a QTL region affecting fatty acid composition, fatness and growth traits. In this study, we evaluated APOA2 as a candidate gene for meat quality traits in an Iberian × Landrace backcross population. The APOA2:c.131T>A polymorphism, located in exon 3 of APOA2 and determining a missense mutation, was associated with the percentage of hexadecenoic acid [C16:1(n–9)], linoleic acid [C18:2(n–6)], α-linolenic acid [C18:3(n–3)], dihomo-gamma-linolenic acid [C20:3(n–6)] and polyunsaturated fatty acids (PUFAs) in backfat. Furthermore, this SNP was associated with the global mRNA expression levels of APOA2 in liver and was used as a marker to determine allelic expression imbalance by pyrosequencing. We determined an overexpression of the T allele in heterozygous samples with a mean ratio of 2.8 (T/A), observing a high variability in the allelic expression among individuals. This result suggests that complex regulatory mechanisms, beyond a single polymorphism (e.g. epigenetic effects or multiple cis-acting polymorphisms), may be regulating APOA2 gene expression., Peer reviewed
 
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DOI: http://hdl.handle.net/10261/269620
Digital.CSIC. Repositorio Institucional del CSIC

HANDLE: http://hdl.handle.net/10261/269620
Digital.CSIC. Repositorio Institucional del CSIC
 
Ver en: http://hdl.handle.net/10261/269620
Digital.CSIC. Repositorio Institucional del CSIC