Dataset.
Analysis of CBK1 mutants in cbk1-deleted cells
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/362411
Digital.CSIC. Repositorio Institucional del CSIC
- Foltman, Magdalena
- Méndez, Iván
- Bech-Serra, Joan J.
- de la Torre, Carolina
- Brace, Jennifer L.
- Weiss, Eric L.
- Lucas, María
- Queralt, Ethel
- Sánchez-Díaz, Alberto
(A) CBK1 cbk1Δ cdc15-2 (YMF3869), (B) cbk1-6E cbk1Δ cdc15-2 (YMF3763), (C) cbk1-5E-E409S cbk1Δ cdc15-2 (YMF4279), (D) cbk1-5E-E574T cbk1Δ cdc15-2 (YMF4280), and (E) cbk1-9A cbk1Δ cdc15-2 (YMF3764) cells were grown in YPD and arrested in late anaphase by raising the temperature to 37 °C before rapamycin was added to half of the culture for 20 min. Subsequently, to allow progression through the cell cycle, cells were released in the absence (i) or presence (ii) of rapamycin. Samples were taken at the indicated times to study cell-cycle progression by flow cytometry. Schematic illustration of cbk1-9A mutant in which phosphosites containing serines or threonines followed by prolines were changed to alanine to block phosphorylations (iii). FACS graphs can be found in the supplementary FACS file (S1 File)., pbio.3002263.s006.pdf, Peer reviewed
DOI: http://hdl.handle.net/10261/362411
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/362411
HANDLE: http://hdl.handle.net/10261/362411
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/362411
Ver en: http://hdl.handle.net/10261/362411
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/362411
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Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/362411
Dataset. 2023
ANALYSIS OF CBK1 MUTANTS IN CBK1-DELETED CELLS
Digital.CSIC. Repositorio Institucional del CSIC
- Foltman, Magdalena
- Méndez, Iván
- Bech-Serra, Joan J.
- de la Torre, Carolina
- Brace, Jennifer L.
- Weiss, Eric L.
- Lucas, María
- Queralt, Ethel
- Sánchez-Díaz, Alberto
(A) CBK1 cbk1Δ cdc15-2 (YMF3869), (B) cbk1-6E cbk1Δ cdc15-2 (YMF3763), (C) cbk1-5E-E409S cbk1Δ cdc15-2 (YMF4279), (D) cbk1-5E-E574T cbk1Δ cdc15-2 (YMF4280), and (E) cbk1-9A cbk1Δ cdc15-2 (YMF3764) cells were grown in YPD and arrested in late anaphase by raising the temperature to 37 °C before rapamycin was added to half of the culture for 20 min. Subsequently, to allow progression through the cell cycle, cells were released in the absence (i) or presence (ii) of rapamycin. Samples were taken at the indicated times to study cell-cycle progression by flow cytometry. Schematic illustration of cbk1-9A mutant in which phosphosites containing serines or threonines followed by prolines were changed to alanine to block phosphorylations (iii). FACS graphs can be found in the supplementary FACS file (S1 File)., pbio.3002263.s006.pdf, Peer reviewed
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