Dataset.

Analysis of intracellular chitin deposition [Dataset]

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337675
Digital.CSIC. Repositorio Institucional del CSIC
  • Giorgio, Ettore de
  • Giannios, Panagiotis
  • Espinàs, Maria Lluïsa
  • Llimargas, Marta
All images show salivary glands. (A, C, E-L, N, O) Show single confocal sections and (B, D, M) show projections of several sections. (A-A’) The concomitant expression of reb and GFP-kkv leads to luminal chitin deposition (blue arrow in orthogonal section in A’). (B, C) Coexpression of expΔMH2 and GFP-kkv produces intracellular chitin punctae, some of which partially colocalise with GFP-Kkv vesicles (yellow arrowhead) while others do not (red arrowhead). GFP-Kkv vesicles without chitin are also observed (green arrowhead). Note the accumulation of chitin in the apical domain (white arrow in C) that is not deposited extracellularly in the lumen (blue arrow in orthogonal section in C’). (D) In Rab5DN background, intracellular chitin punctae are still present (white arrowheads). (E-L) Analysis of the nature of GFP-Kkv vesicles and chitin punctae using markers Golgin245 (E-F), Hrs 27–4 (G-H), Arl8 (I-J), and Rab11 (K-L); arrowheads indicate colocalisation between Kkv and each specific marker. (M, N) All GFP-Kkv vesicles colocalise with the membrane marker CD4-mIFP (white arrowheads), and few of them also with chitin (orange arrowheads); single chitin punctae do not colocalise with CD4-mIFP (red arrowheads). (O-O”) Frames from live imaging movie show that partially colocalising GFP-Kkv and chitin punctae (yellow arrow) can separate from each other; however, many GFP-Kkv (green arrow) and chitin puncta (red arrow) do not colocalise. Scale bars A-D, M: 10 μm; E-L, N-N”‘: 1 μm; O-O”: 5 μm., Peer reviewed
 
DOI: http://hdl.handle.net/10261/337675
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337675

HANDLE: http://hdl.handle.net/10261/337675
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337675
 
Ver en: http://hdl.handle.net/10261/337675
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337675

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/337675
Dataset. 2023

ANALYSIS OF INTRACELLULAR CHITIN DEPOSITION [DATASET]

Digital.CSIC. Repositorio Institucional del CSIC
  • Giorgio, Ettore de
  • Giannios, Panagiotis
  • Espinàs, Maria Lluïsa
  • Llimargas, Marta
All images show salivary glands. (A, C, E-L, N, O) Show single confocal sections and (B, D, M) show projections of several sections. (A-A’) The concomitant expression of reb and GFP-kkv leads to luminal chitin deposition (blue arrow in orthogonal section in A’). (B, C) Coexpression of expΔMH2 and GFP-kkv produces intracellular chitin punctae, some of which partially colocalise with GFP-Kkv vesicles (yellow arrowhead) while others do not (red arrowhead). GFP-Kkv vesicles without chitin are also observed (green arrowhead). Note the accumulation of chitin in the apical domain (white arrow in C) that is not deposited extracellularly in the lumen (blue arrow in orthogonal section in C’). (D) In Rab5DN background, intracellular chitin punctae are still present (white arrowheads). (E-L) Analysis of the nature of GFP-Kkv vesicles and chitin punctae using markers Golgin245 (E-F), Hrs 27–4 (G-H), Arl8 (I-J), and Rab11 (K-L); arrowheads indicate colocalisation between Kkv and each specific marker. (M, N) All GFP-Kkv vesicles colocalise with the membrane marker CD4-mIFP (white arrowheads), and few of them also with chitin (orange arrowheads); single chitin punctae do not colocalise with CD4-mIFP (red arrowheads). (O-O”) Frames from live imaging movie show that partially colocalising GFP-Kkv and chitin punctae (yellow arrow) can separate from each other; however, many GFP-Kkv (green arrow) and chitin puncta (red arrow) do not colocalise. Scale bars A-D, M: 10 μm; E-L, N-N”‘: 1 μm; O-O”: 5 μm., Peer reviewed




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