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Digital.CSIC. Repositorio Institucional del CSIC

oai:digital.csic.es:10261/111563

The dilution hypothesis was tested by adding different concentrations of ambient DOC obtained by solid phase extraction to deep seawater samples. Microbial growth and consumption of DOC were assessed by flow cytometry, HTCO measurements of DOC and oxygen consumption measurements in 14 experiments using water collected from deep water masses of the Atlantic and Pacific Oceans.There are two kinds of experiments 14 (A-N) where prokaryotic growth was evaluated under increasing concentrations of ambient DOC and 2 additional experiments (O and P) where DOC composition and the utilization of different compounds was evaluated by means of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS). A utilization index for each compound was derived from the FT-ICR-MS fingerprints, showing whether the relative signal for each compound remained stable (refractory or not used), decreased (was consumed) or increased (was produced). Detailed information on conditions and procedures can be found in the article. Enquiries can be sent to Jesús M. Arrieta at txetxu[at]mail.com., Access and reuse conditions: This database and its components are subject to a Creative Commons Attribution-Noncommercial-ShareAlike International licence 4.0., Experimental results on the hypothesis that deep-water DOC consists of many different, intrinsically labile compounds at concentrations too low to compensate for the metabolic costs associated to their utilization., This is a contribution to the MALASPINA Expedition 2010 project, funded by the CONSOLIDER-Ingenio 2010 program of the Spanish Ministry of Economy and Competitiveness (Ref. CSD2008-00077). J.M.A. was supported by a “Ramón y Cajal” research fellowship from the Spanish Ministry of Economy and Competitiveness. E.M. was supported by a fellowship from the JAE program of CSIC. G.J.H. and R.H. were supported by the Austrian Science Fund (FWF) projects: I486-B09 and P23234-B11 and by the European Research Council under the European Community’s Seventh Framework Programme (FP7/2007-2013) / ERC grant agreement No. 268595 (MEDEA project). We thank A. Dorsett for assistance with DOC analyses, participants in the Malaspina Expedition and the crews of the BIO Hespérides, and RV Pelagia and the personnel of the Marine Technology Unit of CSIC (UTM) for their invaluable support., Peer reviewed