. 2022

High throughput stationary 13C-Metabolic Flux Analysis of 3-hydroxypropionic acid producing Pichia pastoris strains. Supplementary materials of Chapter 5 of the PhD Thesis of Albert Fina Romero

Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:264385
Dipòsit Digital de Documents de la UAB
  • Fina Romero, Albert|||0000-0001-7949-0484
  • Albiol i Sala, Joan|||0000-0001-5626-429X
  • Ferrer, Pau|||0000-0002-5287-4127
Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis).
 

DOI: https://ddd.uab.cat/record/264385, https://dx.doi.org/10.5565/ddd.uab.cat/264385
Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:264385

HANDLE: https://ddd.uab.cat/record/264385, https://dx.doi.org/10.5565/ddd.uab.cat/264385
Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:264385
 
Ver en: https://ddd.uab.cat/record/264385, https://dx.doi.org/10.5565/ddd.uab.cat/264385
Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:264385

Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:264385
. 2022

HIGH THROUGHPUT STATIONARY 13C-METABOLIC FLUX ANALYSIS OF 3-HYDROXYPROPIONIC ACID PRODUCING PICHIA PASTORIS STRAINS. SUPPLEMENTARY MATERIALS OF CHAPTER 5 OF THE PHD THESIS OF ALBERT FINA ROMERO

Dipòsit Digital de Documents de la UAB
  • Fina Romero, Albert|||0000-0001-7949-0484
  • Albiol i Sala, Joan|||0000-0001-5626-429X
  • Ferrer, Pau|||0000-0002-5287-4127
Supplementary files of Chapter 5 of the PhD Thesis of Albert Fina Romero. This chapter describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis).