Publicación Artículo científico (article).

A Phase I Randomized Therapeutic MVA-B Vaccination Improves the Magnitude and Quality of the T Cell Immune Responses in HIV-1-Infected Subjects on HAART

Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:163254
Dipòsit Digital de Documents de la UAB
  • Gómez, Carmen Elena
  • Perdiguero, Beatriz
  • García-Arriaza, Juan
  • Cepeda, Victoria
  • Sánchez-Sorzano, Carlos Óscar
  • Mothe, Beatriz|||0000-0001-9975-407X
  • Jiménez, José Luis
  • Muñoz-Fernández, María Ángeles
  • Gatell, José María
  • López Bernaldo de Quirós, Juan Carlos
  • Brander, Christian|||0000-0002-0548-5778
  • García, Felipe|||0000-0001-7658-5832
  • Esteban, Mariano
TRIAL DESIGN: Previous studies suggested that poxvirus-based vaccines might be instrumental in the therapeutic HIV field. A phase I clinical trial was conducted in HIV-1-infected patients on highly active antiretroviral therapy (HAART), with CD4 T cell counts above 450 cells/mm3 and undetectable viremia. Thirty participants were randomized (2:1) to receive either 3 intramuscular injections of MVA-B vaccine (coding for clade B HIV-1 Env, Gag, Pol and Nef antigens) or placebo,followed by interruption of HAART. METHODS: The magnitude, breadth, quality and phenotype of the HIV-1-specific T cell response were assayed by intracellular cytokine staining (ICS) in 22 volunteers pre- and post-vaccination. RESULTS: MVA-B vaccine induced newly detected HIV-1-specific CD4 T cell responses and expanded pre-existing responses (mostly against Gag, Pol and Nef antigens) that were high in magnitude, broadly directed and showed an enhanced polyfunctionality with a T effector memory (TEM) phenotype, while maintaining the magnitude and quality of the pre-existing HIV-1-specific CD8 T cell responses. In addition, vaccination also triggered preferential CD8+ T cell polyfunctional responses to the MVA vector antigens that increase in magnitude after two and three booster doses. CONCLUSION: MVA-B vaccination represents a feasible strategy to improve T cell responses in individuals with pre-existing HIV-1-specific immunity. TRIAL REGISTRATION: ClinicalTrials. gov NCT01571466.
 

DOI: https://ddd.uab.cat/record/163254, https://dx.doi.org/urn:doi:10.1371/journal.pone.0141456
Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:163254

HANDLE: https://ddd.uab.cat/record/163254, https://dx.doi.org/urn:doi:10.1371/journal.pone.0141456
Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:163254
 
Ver en: https://ddd.uab.cat/record/163254, https://dx.doi.org/urn:doi:10.1371/journal.pone.0141456
Dipòsit Digital de Documents de la UAB
oai:ddd.uab.cat:163254