Dataset.

Osteosarcoma tissue-engineered model challenges oxidative stress therapy revealing promoted cancer stem cell properties [Dataset]

UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/184325
UPCommons. Portal del coneixement obert de la UPC
  • Tornin Cavielles, Juan|||0000-0002-7676-3958
  • Villasante, Aranzazu
  • Sole Marti, Xavi
  • Ginebra Molins, Maria Pau|||0000-0002-4700-5621
  • Canal Barnils, Cristina|||0000-0002-3039-7462
These data are associated to the paper with the same title of the authors, and are structured according to the figures in the paper: http://hdl.handle.net/2117/335727, The use of Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects of CAP for treating osteosarcoma have mostly been demonstrated in 2-dimensional cultures of cells, which do not mimic the complexity of the 3-dimensional (3D) bone microenvironment. In order to evaluate the effects of CAP in a relevant context of the human disease, we developed a 3D tissue-engineered model of osteosarcoma using a bone-like scaffold made of collagen type I and hydroxyapatite nanoparticles. Human osteosarcoma cells cultured within the scaffold showed a high capacity to infiltrate and proliferate and to exhibit osteomimicry in vitro. As expected, we observed significantly different functional behaviors between monolayer and 3D cultures when treated with Cold Plasma-Activated Ringer’s Solution (PAR). Our data reveal that the 3D environment not only protects cells from PAR-induced lethality by scavenging and diminishing the amount of reactive oxygen and nitrogen species generated by CAP, but also favours the stemness phenotype of osteosarcoma cells. This is the first study that demonstrates the negative effect of PAR on cancer stem-like cell subpopulations in a 3D biomimetic model of cancer. These findings will allow to suitably re-focus research on plasma-based therapies in future
 

DOI: http://hdl.handle.net/2117/184325, https://dx.doi.org/10.5821/data-2117-184325-1
UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/184325

HANDLE: http://hdl.handle.net/2117/184325, https://dx.doi.org/10.5821/data-2117-184325-1
UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/184325
 
Ver en: http://hdl.handle.net/2117/184325, https://dx.doi.org/10.5821/data-2117-184325-1
UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/184325

r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
oai:fundanet.fsjd.org:p18655
Artículo científico (article). 2021

OSTEOSARCOMA TISSUE-ENGINEERED MODEL CHALLENGES OXIDATIVE STRESS THERAPY REVEALING PROMOTED CANCER STEM CELL PROPERTIES.

r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
  • Tornín J
  • Villasante A
  • Solé-Martí X
  • Ginebra MP
  • Canal C
The use of oxidative stress generated by Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects of CAP for treating osteosarcoma have mostly been demonstrated in 2-dimensional cultures of cells, which do not mimic the complexity of the 3-dimensional (3D) bone microenvironment. In order to evaluate the effects of CAP in a relevant context of the human disease, we developed a 3D tissue-engineered model of osteosarcoma using a bone-like scaffold made of collagen type I and hydroxyapatite nanoparticles. Human osteosarcoma cells cultured within the scaffold showed a high capacity to infiltrate and proliferate and to exhibit osteomimicry in vitro. As expected, we observed significantly different functional behaviors between monolayer and 3D cultures when treated with Cold Plasma-Activated Ringer's Solution (PAR). Our data reveal that the 3D environment not only protects cells from PAR-induced lethality by scavenging and diminishing the amount of reactive oxygen and nitrogen species generated by CAP, but also favours the stemness phenotype of osteosarcoma cells. This is the first study that demonstrates the negative effect of PAR on cancer stem-like cell subpopulations in a 3D biomimetic model of cancer. These findings will allow to suitably re-focus research on plasma-based therapies in future.



UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/335727
Artículo científico (article). 2021

OSTEOSARCOMA TISSUE-ENGINEERED MODEL CHALLENGES OXIDATIVE STRESS THERAPY REVEALING PROMOTED CANCER STEM CELL PROPERTIES

UPCommons. Portal del coneixement obert de la UPC
  • Tornin Cavielles, Juan|||0000-0002-7676-3958
  • Villasante Bermejo, Aránzazu
  • Solé Martí, Xavier|||0000-0002-5544-2485
  • Ginebra Molins, Maria Pau|||0000-0002-4700-5621
  • Canal Barnils, Cristina|||0000-0002-3039-7462
The use of oxidative stress generated by Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects of CAP for treating osteosarcoma have mostly been demonstrated in 2-dimensional cultures of cells, which do not mimic the complexity of the 3-dimensional (3D) bone microenvironment. In order to evaluate the effects of CAP in a relevant context of the human disease, we developed a 3D tissue-engineered model of osteosarcoma using a bone-like scaffold made of collagen type I and hydroxyapatite nanoparticles. Human osteosarcoma cells cultured within the scaffold showed a high capacity to infiltrate and proliferate and to exhibit osteomimicry in vitro. As expected, we observed significantly different functional behaviors between monolayer and 3D cultures when treated with Cold Plasma-Activated Ringer's Solution (PAR). Our data reveal that the 3D environment not only protects cells from PAR-induced lethality by scavenging and diminishing the amount of reactive oxygen and nitrogen species generated by CAP, but also favours the stemness phenotype of osteosarcoma cells. This is the first study that demonstrates the negative effect of PAR on cancer stem-like cell subpopulations in a 3D biomimetic model of cancer. These findings will allow to suitably re-focus research on plasma-based therapies in future., Peer Reviewed





UPCommons. Portal del coneixement obert de la UPC
oai:upcommons.upc.edu:2117/184325
Dataset. 2020

OSTEOSARCOMA TISSUE-ENGINEERED MODEL CHALLENGES OXIDATIVE STRESS THERAPY REVEALING PROMOTED CANCER STEM CELL PROPERTIES [DATASET]

UPCommons. Portal del coneixement obert de la UPC
  • Tornin Cavielles, Juan|||0000-0002-7676-3958
  • Villasante, Aranzazu
  • Sole Marti, Xavi
  • Ginebra Molins, Maria Pau|||0000-0002-4700-5621
  • Canal Barnils, Cristina|||0000-0002-3039-7462
These data are associated to the paper with the same title of the authors, and are structured according to the figures in the paper: http://hdl.handle.net/2117/335727, The use of Cold Atmospheric Plasma (CAP) in oncology is being recently studied as a novel potential anti-cancer therapy. However, the beneficial effects of CAP for treating osteosarcoma have mostly been demonstrated in 2-dimensional cultures of cells, which do not mimic the complexity of the 3-dimensional (3D) bone microenvironment. In order to evaluate the effects of CAP in a relevant context of the human disease, we developed a 3D tissue-engineered model of osteosarcoma using a bone-like scaffold made of collagen type I and hydroxyapatite nanoparticles. Human osteosarcoma cells cultured within the scaffold showed a high capacity to infiltrate and proliferate and to exhibit osteomimicry in vitro. As expected, we observed significantly different functional behaviors between monolayer and 3D cultures when treated with Cold Plasma-Activated Ringer’s Solution (PAR). Our data reveal that the 3D environment not only protects cells from PAR-induced lethality by scavenging and diminishing the amount of reactive oxygen and nitrogen species generated by CAP, but also favours the stemness phenotype of osteosarcoma cells. This is the first study that demonstrates the negative effect of PAR on cancer stem-like cell subpopulations in a 3D biomimetic model of cancer. These findings will allow to suitably re-focus research on plasma-based therapies in future





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