BIOSINTESIS DE AMINOACIDOS Y ECONOMIA DEL NITROGENO EN PLANTAS DE INTERES FORESTAL
BIO2015-69285-R
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Nombre agencia financiadora Ministerio de Economía y Competitividad
Acrónimo agencia financiadora MINECO
Programa Programa Estatal de I+D+I Orientada a los Retos de la Sociedad
Subprograma Todos los retos
Convocatoria Proyectos de I+D+I dentro del Programa Estatal Retos de la Sociedad (2015)
Año convocatoria 2015
Unidad de gestión Dirección General de Investigación Científica y Técnica
Centro beneficiario UNIVERSIDAD DE MALAGA
Centro realización DPTO. BIOLOGIA MOLECULAR Y BIOQUIMICA
Identificador persistente http://dx.doi.org/10.13039/501100003329
Publicaciones
Found(s) 1 result(s)
Found(s) 1 page(s)
Found(s) 1 page(s)
Overexpression of a pine Dof transcription factor in hybrid poplars: A comparative study in trees growing under controlled and natural conditions
Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
- Rueda López, Marina
- Pascual, María Belén
- Pallero, Mercedes
- Henao, Luisa María
- Lasa Larrea, Berta
- Jáuregui Mosquera, Iván
- Aparicio Tejo, Pedro María
- Cánovas, Francisco M.
- Ávila, Concepción
In this work, the role of the pine transcriptional regulator Dof 5 in carbon and nitrogen metabolism
has been examined in poplar trees. The overexpression of the gene and potential
effects on growth and biomass production were compared between trees growing in a
growth chamber under controlled conditions and trees growing in a field trial during two
growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed
controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form
of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates
such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of
the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin
were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key
enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However,
the results in the field trial experiment diverged from those observed in the chamber
system. The lines overexpressing PpDof5 showed attenuated growth during the two growing
seasons and no modification of carbon or nitrogen metabolism. These results were not
associated with a decrease in the expression of the transgene, but they can be ascribed to
the nitrogen available in the field soil compared to that available for growth under controlled
conditions. This work highlights the paramount importance of testing transgenic lines in field
trials., This work was supported by grants from the Spanish Ministerio de Economía y Competitividad (BIO2015-69285-R) and Junta de Andalucia (BIO-474).
has been examined in poplar trees. The overexpression of the gene and potential
effects on growth and biomass production were compared between trees growing in a
growth chamber under controlled conditions and trees growing in a field trial during two
growth seasons. Ten-week-old transgenic poplars exhibited higher growth than untransformed
controls and exhibited enhanced capacity for inorganic nitrogen uptake in the form
of nitrate. Furthermore, the transgenic trees accumulated significantly more carbohydrates
such as glucose, fructose, sucrose and starch. Lignin content increased in the basal part of
the stem likely due to the thicker stem of the transformed plants. The enhanced levels of lignin
were correlated with higher expression of the PAL1 and GS1.3 genes, which encode key
enzymes involved in the phenylalanine deamination required for lignin biosynthesis. However,
the results in the field trial experiment diverged from those observed in the chamber
system. The lines overexpressing PpDof5 showed attenuated growth during the two growing
seasons and no modification of carbon or nitrogen metabolism. These results were not
associated with a decrease in the expression of the transgene, but they can be ascribed to
the nitrogen available in the field soil compared to that available for growth under controlled
conditions. This work highlights the paramount importance of testing transgenic lines in field
trials., This work was supported by grants from the Spanish Ministerio de Economía y Competitividad (BIO2015-69285-R) and Junta de Andalucia (BIO-474).
Proyecto: MINECO//BIO2015-69285-R