BUSCADOR RECOLECTA

During the sampling period from 29/10/2018 to 31/01/2020, traps were installed inside the municipal facilities in the City of Barcelona. 111 Mus musculus domesticus simples were collected and a tail fragment was taken and stored at -20ºC for subsequent molecular analysis. We extracten genomic DNA and analyzed the mutations in the Vkorc1 gene in exon 1, exon 2 and exon 3 that are 174 bp, 110 bp and 202 bp long, respectively, and encode in total 161 amino acids., Agència de Salut Pública de Barcelona., Peer reviewed

Telomere length of magpie and great spotted cuckoo nestlings Telomere length of magpie and great spotted cuckoo nestlings soon after hatching and before fledging. We also show telomere attrition values and laying-date of each studied nest. Values are means estimates per study nest. data.xlsx, Although little is known on the impact of environment on telomere length dynamics, it has been suggested to be affected by stress, lifestyle and/or life-history strategies of animals. We here compared telomere dynamics in erythrocytes of hatchlings and fledglings of the brood parasite great spotted cuckoos (Clamator glandarius) and of magpies (Pica pica), their main host in Europe. In magpie chicks, telomere length decreased from hatching to fledging while no significant change in telomere length of great spotted cuckoo chicks was found. Moreover, we found interspecific differences in the association between laying date and telomere shortening. Interspecific differences in telomere shortening were interpreted as a consequence of differences in lifestyle and life-history characteristics of magpies and great spotted cuckoos. In comparison with magpies, cuckoos experience reduced sibling competition and higher access to resources, and, consequently, lower stressful environmental conditions during the nestling phase. These characteristics also explain the associations between telomere attrition and environmental conditions (i.e. laying date) for magpies and the absence of association for great spotted cuckoos. These results therefore fit expectations on telomere dynamics derived from interspecific differences in lifestyle and life-history of brood parasites and their bird hosts., Peer reviewed

[Usage Notes] Dataset. Dataset used in the analysis of breeding output of the color polymorphic Eleonora's falcon., Morph-specific mate choice has been proposed as one of the evolutionary mechanisms that contribute to the maintenance of variation in color polymorphic systems. Coloration usually covaries with other phenotypic traits affecting life history and thus is often used as a criterion for mate choice. Here, we assess whether mating patterns, natal dispersal, and breeding output are phenotype-dependent in the color polymorphic Eleonora’s falcon. We used a long-term dataset of 946 individually ringed adult falcons that included 109 individuals monitored from birth up to recruitment into the breeding population. Overall, patterns of mate choice with regard to coloration were neither assortative nor disassortative. Natal dispersal distance was greater in females but was not associated with coloration. Breeding success was both morph-dependent and context-dependent. Although clutch size was similar in differently colored pairs, differences arose in the number of chicks that fledge. In some years, dark males raised more offspring, regardless of female color morph. Differences in the breeding tactics between male morphs could be associated with intraspecific predation and may thus contribute to the observed differences in breeding output, especially when food availability is low. This suggests that mating patterns may interact with other factors and give rise to the observed higher breeding output of dark males only under certain environmental conditions., Peer reviewed

Telomeres are repetitive DNA sequences at the end of chromosomes that protect them from degradation. They have been the focus of intense research because short telomeres would predict accelerated ageing and reduced longevity in vertebrates. Oxidative stress is considered a physiological driver of the telomere shortening and, consequently, short lifespan. Among molecules fighting against oxidative stress, glutathione is involved in many antioxidant pathways. Literature supports that oxidative stress may trigger a compensatory “hormetic” response increasing glutathione levels and telomere length. Here we tested the link between total glutathione concentration and telomere length in captive birds (zebra finches; Taeniopygia guttata). Total glutathione levels were experimentally decreased during birds’ growth using a specific inhibitor of glutathione synthesis (buthionine sulfoximine; BSO). We monitored the birds’ reproductive performance in an outdoor aviary during the first months of life and their almost nine-year longevity. We predicted that if glutathione protects telomeres from oxidative stress-induced telomere attrition, a positive correlation between telomere length and glutathione concentration in erythrocytes of control individuals should emerge. Similarly, we predicted that since BSO-treated zebra finches endured higher oxidative stress than controls, they should have also developed shorter telomeres in their erythrocytes and reduced longevity and reproductive outcomes. The current dataset allows testing these predictions., Financial support was obtained from projects CGL-2009-10883-C02-02, CGL2012-40229-C02-01 and CGL2015-69338-C2-2-P and PID2019-109303GB-I00 from Ministerio de Economía y Competitividad, MINECO, Spanish Government., Peer reviewed

[Description of methods used for collection/generation of data] We collected birds infected by Plasmodium cathemerium and Plasmodium relictum and uninfected birds. We collected mosquito larvae that were raised in the lab. Adult mosquitoes were divided in 3 groups allowed them to feed overnight on a P. relictum-infected bird, a P. cathemerium-infected bird and an uninfected control bird. For transcriptome analyses, we processed mosquitoes at three time points after exposure, 24 hours post-infection (hpi), 10 days post-infection (dpi) and 21 dpi. At each time point, we created pools of 5 mosquitoes of each infection status capturing the mosquitoes alive and immediately transferring them to dry ice. We preserved the mosquitoes at -80ºC until RNA extractions were carried out. We collected a total of 36 samples including controls (4 pools x 3 time points x 3 conditions). We extracted RNA and DNA from pools of 5 mosquitoes using TRIzol® (Invitrogen, Carlsbad, CA, USA) followed by column purification using RNeasy mini kit® (QIAGEN, Hilden, Germany). RNA samples were submitted to the Polo d’Innovazione di Genomica, Genetica e Biologia, Siena (Italy) where library preparation and sequencing were carried out., [Methods for processing the data] Illumina sequencing and RNAseq pipeline available with the published paper., Metadata describing the experimental conditions of an RNA seq experiment that aims to analyse the differential gene expression of Culex pipiens when exposed to either Plasmodium relictum or Plasmodium cathemerium. The data includes the information of the corresponding project published at the European Nucleotide Archive (https://www.ebi.ac.uk/ena/browser/view/PRJEB41609), sample IDs and the links to the generated fasta files., This publication was supported by the project Research Infrastructures for the control of vector-borne diseases (Infravec2), which has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 731060., Peer reviewed

Black-winged Stilt chicks were captured during the breeding season (from April to August) from 2005-2011 in Doñana rice fields located near the Guadalquivir marshes (37º09N 06º08W, SW Spain), in 2009-2010 in rice fields of Sidi Allal Tazi and in 2009-2011 in rice fields of Larache (both sites in NW Morocco, 34º30N 6º16W and 35º11N 6º07W respectively). Birds were also sampled at natural ponds in the Doñana area in 2010-2011 (37º04N 6º27W), and in natural wetlands at Larache and a coastal lagoon in Briech (NW Morocco, 35º11N 6º07W and 35º31N 6º00W respectively) in 2011., The Junta de Andalucía funded this study through the project “Las aves acuáticas de Doñana y el cultivo del arroz: la interacción entre la agricultura y la conservación de las zonas húmedas”. This study was possible thanks to the INRA administration and CNRST-CSIC collaboration program (P2007MA01 and P2009MA01). GMT was funded by an I3P-CSIC grant for the formation of Researchers. MMH is supported by a Marie Curie Intra-European Fellowship for Career Development (PIEF-GA-2011-299747) within the 7th Framework Programme (FP7 2007-2013) of the European Commission., Peer reviewed

Data was collected by Juli Broggi in la Cañada de los Pajaros, Puebla del Rio (Sevilla, Spain), and later analyzed at EBD (Sevilla,) following a detailed experimental design (Spring-summer 2010), Peer reviewed

Nest materials used by animals can have profound effects on developing offspring. They can modify the bacterial and parasitic environment of the nest, and can influence parental investment through sexual signalling processes. In spotless starlings, Sturnus unicolor, green plants and feathers are known nest materials with such functions. The aim of our study was to experimentally assess their influence on nestlings’ telomere length and attrition, which are good predictors of their survival prospects. In a full-factorial experiment, we explored these effects in two different populations, together with the potential effects of hatching date, ectoparasitism, bacterial environment and nestling growth. Telomere length and attrition largely depended on population identity and hatching date. After correcting for these effects, the addition of feathers resulted in higher rates of telomere attrition. The addition of plants did not affect nestling telomeres in general, but did in interaction with location: in Hueneja, the experimental addition of green plants resulted in longer telomeres. Feather pigmentation also did not affect telomere length or attrition in general, but did in interaction with location: in Hueneja, the experimental addition of unpigmented feathers resulted in nestlings with longer telomeres and lower attrition rates. Moreover, prevalence of staphylococci on the skin of 8-day-old nestlings was negatively related to telomere lengths of fledglings. Taken together, these results suggest a direct link between nest material composition and nestling telomere length and dynamics. This relationship could be partially mediated by the antimicrobial and/or antiparasitic properties of nest materials or by sexual signalling, Funding was provided by the Spanish Ministerio de Economia y Competitividad and European funds (FEDER) (CGL2013-48193-C3-1-P, CGL2013-48193-C3-2-P, CGL2012-30759, CGL2015-65055-P). J.M.P., G.T. and M.R.R. were supported respectively by Juan de la Cierva, Ramón & Cajal and Talentia postdoctoral contracts., Peer reviewed

Global warming and direct anthropogenic impacts, such as water extraction, are largely affecting water budgets in Mediterranean wetlands, thereby increasing wetland salinities and isolation, and decreasing water depths and hydroperiods (duration of the inundation period). These wetland features are key elements structuring waterbird communities. However, the ultimate and net consequences of these dynamic conditions on waterbird assemblages are largely unknown. We combined a regular sampling on waterbird presence through the 2008 annual cycle with in-situ data on these relevant environmental predictors of waterbird distribution to model habitat selection for 69 individual species in a typical Mediterranean wetland network in south-western Spain. Species association with environmental features were subsequently used to predict changes in habitat suitability for each species under three climate change scenarios (encompassing changes in environment that ranged from 10% to 50% change as predicted by climatic models). Waterbirds distributed themselves unevenly throughout environmental gradients and water salinity was the most important gradient structuring the distribution of the community. Environmental suitability for the guilds of diving birds and vegetation gleaners will be reduced according to future climate scenarios, while most small wading birds will benefit from changing conditions. Resident species and those that breed in this wetland network will be also more impacted than those using this area for wintering or stopover. We provide here a tool that can be used in a horizon-scanning framework to identify emerging issues on waterbird conservation and to anticipate suitable management actions, Peer reviewed

En la tabla se indican características de los nidos y pollos de estornino negro (Sturnus unicolor) utilizados en el artículo: Juan J. Soler, Cristina Ruiz-Castellano, Jordi Figuerola, Josué Martínez-de la Puente, Magdalena Ruiz-Rodríguez and Gustavo Tomás (2018) Egg colouration predicts brood size, telomere length and body condition of spotless starling fledglings. Journal of Avian Biology, e01686. doi: 10.1111/jav.01686 Nest identity: identidad de los nidos utilizados . feather treatment: si se añadieron plumas a los nidos durante el crecimiento de los pollos o no. plant treatment: si se añadieron plantas verdes a los nidos durante el crecimiento de los pollos o no. Brightness: Brillo (luminosidad) de los huevos de estorninos de los distintos nidos. Blue-Green chroma (400-580): Croma verde-azulado, reflectancia en longitudes de onda. comprendidas entre 400 y 580 nm dividida por la reflectanca de todo el espectro (300-700nm) en tanto por ciento. hathing date (1=1st april): fecha de eclosion (el valor 1 corresponde al 1 de abril). Clutch size: tamaño de puesta. Brood size: tamaño de pollada. Body mass: peso corporal de pollos de 14 dias de edad. Tarsus length: longitude del tarso de pollos de 14 días de edad. Body condition: condicion corporal de pollos de 14 días de edad estimada como los residuos del peso después de controlar por la longitud del tarso. Telomere length (hatchlings): longitud de los telómeros de pollos recién eclosionados. Telomere length (fledglings): longitude de los telómeros de pollos poco antes de abandonar el nido., Para la recogida de los datos se ha seguido la reproducción de dos poblaciones de estornino negro. Además de anotar información sobre distintas variables reproductoras (fecha de puesta, taño de puesta y de pollada,, y éxito reproductor, se ha medido el color de sus huevos, se han tomado muestras de sangre de pollos recién eclosionados y poco antes de abandonar el nido. También se midió el tarso y el peso corporal de los pollos poco antes de abandonar el nido. La estima del tamaño de los telomeros se realizó en el laboratorio, a partir de ADN de los glóbulos rojos de las muestras de sangre recogidas en el campo., Funding was provided by the Spanish Ministerio de Economia y Competitividad and European funds (FEDER) (CGL2013-48193-C3-1-P, CGL2013-48193-C3-2-P, CGL2012-30759, CGL2015-65055-P). JMP, GT and MRR were supported by Juan de la Cierva, Ramón & Cajal and Talentia postdoctoral contracts, respectively. JMP was partially supported by a 2017 Leonardo Grant for Researchers and Cultural Creators, BBVA Foundation., Peer reviewed