Resultados totales (Incluyendo duplicados): 1933
Encontrada(s) 194 página(s)
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/345717
. 2017

SAFETY AND VACCINE-INDUCED HIV-1 IMMUNE RESPONSES IN HEALTHY VOLUNTEERS FOLLOWING A LATE MVA-B BOOST 4 YEARS AFTER THE LAST IMMUNIZATION

  • Guardo, Alberto C
  • Gómez, Carmen Elena
  • Díaz-Brito, Vicens
  • Pich, Judit
  • Arnaiz, Joan Albert
  • Perdiguero, Beatriz
  • GARCIA ARRIAZA, JUAN FRANCISCO
  • González, Nuria
  • Sorzano, Carlos O S
  • Jiménez, Laura
  • Jiménez, José Luis
  • Muñoz-Fernández, María Ángeles
  • Gatell, José M
  • Alcamí, José
  • Esteban, Mariano
  • López Bernaldo de Quirós, Juan Carlos
  • García, Felipe
  • Plana, Montserrat
We have previously shown that an HIV vaccine regimen including three doses of HIV-modified vaccinia virus Ankara vector expressing HIV-1 antigens from clade B (MVA-B) was safe and elicited moderate and durable (1 year) T-cell and antibody responses in 75% and 95% of HIV-negative volunteers (n = 24), respectively (RISVAC02 study). Here, we describe the long-term durability of vaccine-induced responses and the safety and immunogenicity of an additional MVA-B boost., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/345717, https://api.elsevier.com/content/abstract/scopus_id/85032215204
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/345717
HANDLE: http://hdl.handle.net/10261/345717, https://api.elsevier.com/content/abstract/scopus_id/85032215204
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/345717
PMID: http://hdl.handle.net/10261/345717, https://api.elsevier.com/content/abstract/scopus_id/85032215204
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/345717
Ver en: http://hdl.handle.net/10261/345717, https://api.elsevier.com/content/abstract/scopus_id/85032215204
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/345717

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/321612
Sound. 2017

MAITOTOXIN-4, A NOVEL MTX ANALOG PRODUCED BY GAMBIERDISCUS EXCENTRICUS

  • Pisapia, Francesco
  • Sibat, Manoëlla
  • Herrenknecht, Christine
  • Lhaute, Korian
  • Gaiani, Greta
  • Ferron, Pierre-Jean
  • Fessard, Valérie
  • Fraga, Santiago
  • Nascimento, Silvia Mattos
  • Litaker, Wayne
  • Holland, W.C.
  • Roullier, Catherine
  • Hess, Philipp
Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

Proyecto: //
DOI: http://hdl.handle.net/10508/11307, http://hdl.handle.net/10261/321612
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/321612
HANDLE: http://hdl.handle.net/10508/11307, http://hdl.handle.net/10261/321612
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/321612
PMID: http://hdl.handle.net/10508/11307, http://hdl.handle.net/10261/321612
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/321612
Ver en: http://hdl.handle.net/10508/11307, http://hdl.handle.net/10261/321612
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/321612

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/323438
Sound. 2017

MAITOTOXIN-4, A NOVEL MTX ANALOG PRODUCED BY GAMBIERDISCUS EXCENTRICUS

  • Pisapia, Francesco
  • Sibat, Manoëlla
  • Herrenknecht, Christine
  • Lhaute, Korian
  • Gaiani, Greta
  • Ferron, Pierre-Jean
  • Fessard, Valérie
  • Fraga, Santiago
  • Nascimento, Silvia Mattos
  • Litaker, Wayne
  • Holland, W.C.
  • Roullier, Catherine
  • Hess, Philipp
Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

Proyecto: //
DOI: http://hdl.handle.net/10508/11307, http://hdl.handle.net/10261/323438
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/323438
HANDLE: http://hdl.handle.net/10508/11307, http://hdl.handle.net/10261/323438
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/323438
PMID: http://hdl.handle.net/10508/11307, http://hdl.handle.net/10261/323438
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/323438
Ver en: http://hdl.handle.net/10508/11307, http://hdl.handle.net/10261/323438
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/323438

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/324823
Sound. 2017

ESPAÑOLES EN LA MAR - MAR DEL NORTE - 20/02/17 20 FEB 2017

  • Llope, Marcos
El Mar del Norte alberga importantes bancos de pesca. Es un ecosistema diverso en el que se producen complejas interacciones en la cadena trófica que han sido objeto de estudio por un grupo de científicos de distintos países entre los que se encuentra Marcos Llope, del Instituto Español de Oceanografía. Con él hablamos en nuestro viaje de hoy.

Proyecto: //
DOI: http://hdl.handle.net/10508/10799, http://hdl.handle.net/10261/324823
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/324823
HANDLE: http://hdl.handle.net/10508/10799, http://hdl.handle.net/10261/324823
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/324823
PMID: http://hdl.handle.net/10508/10799, http://hdl.handle.net/10261/324823
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/324823
Ver en: http://hdl.handle.net/10508/10799, http://hdl.handle.net/10261/324823
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/324823

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/281357
Dataset. 2017

DATA FROM: TACKLING INTRASPECIFIC GENETIC STRUCTURE IN DISTRIBUTION MODELS BETTER REFLECTS SPECIES GEOGRAPHICAL RANGE

  • Marcer, Arnald
  • Méndez-Vigo, Belén
  • Alonso-Blanco, Carlos
  • Picó, F. Xavier
Arabidopsis_data_279accessions_20jan16, Genetic diversity provides insight into heterogeneous demographic and adaptive history across organisms’ distribution ranges. For this reason, decomposing single species into genetic units may represent a powerful tool to better understand biogeographical patterns as well as improve predictions of the effects of GCC (global climate change) on biodiversity loss. Using 279 georeferenced Iberian accessions, we used classes of three intraspecific genetic units of the annual plant Arabidopsis thaliana obtained from the genetic analyses of nuclear SNPs (single nucleotide polymorphisms), chloroplast SNPs, and the vernalization requirement for flowering. We used SDM (species distribution models), including climate, vegetation, and soil data, at the whole-species and genetic-unit levels. We compared model outputs for present environmental conditions and with a particularly severe GCC scenario. SDM accuracy was high for genetic units with smaller distribution ranges. Kernel density plots identified the environmental variables underpinning potential distribution ranges of genetic units. Combinations of environmental variables accounted for potential distribution ranges of genetic units, which shrank dramatically with GCC at almost all levels. Only two genetic clusters increased their potential distribution ranges with GCC. The application of SDM to intraspecific genetic units provides a detailed picture on the biogeographical patterns of distinct genetic groups based on different genetic criteria. Our approach also allowed us to pinpoint the genetic changes, in terms of genetic background and physiological requirements for flowering, that Iberian A. thaliana may experience with a GCC scenario applying SDM to intraspecific genetic units., Peer reviewed

Proyecto: //

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/281358
Dataset. 2017

DATA FROM: GENETIC AND EPIGENETIC DIVERGENCE BETWEEN DISTURBED AND UNDISTURBED SUBPOPULATIONS OF A MEDITERRANEAN SHRUB: A 20-YEAR FIELD EXPERIMENT

  • Herrera, Carlos M.
  • Bazaga, Pilar
AFLP profiles for the plants of Lavandula latifolia sampled for the study Markers are identified by primer combination and size (base pairs) AFLP.scores.xlsx MSAP profiles for the plants of Lavandula latifolia sampled for the study Markers are identified by primer combination and size (base pairs). MSAP.scores.xlsx Spatial coordinates of Lavandula latifolia plants sampled for the study Coordinates are expressed in meters and the origin (0,0) is the lower left corner of the plot as depicted in the figures. Plant.coordinates.xlsx, Little is known on the potential of ecological disturbance to cause genetic and epigenetic changes in plant populations. We take advantage of a long-term field experiment initiated in 1986 to study the demography of the shrub Lavandula latifolia, and compare genetic and epigenetic characteristics of plants in two adjacent subplots, one experimentally disturbed and one left undisturbed, 20 years after disturbance. Experimental setup was comparable to an unreplicated ‘Before-After-Control-Impact’ (BACI) design where a single pair of perturbed and control areas were compared. When sampled in 2005, plants in the two subplots had roughly similar ages, but they had established in contrasting environments: dense conspecific population (‘Undisturbed’ subpopulation) versus open area with all conspecifics removed (‘Disturbed’ subpopulation). Plants were characterized genetically and epigenetically using amplified fragment length polymorphism (AFLP) and two classes of methylation-sensitive AFLP (MSAP) markers. Subpopulations were similar in genetic diversity but differed in epigenetic diversity and multilocus genetic and epigenetic characteristics. Epigenetic divergence between subpopulations was statistically unrelated to genetic divergence. Bayesian clustering revealed an abrupt linear boundary between subpopulations closely coincident with the arbitrary demarcation line between subplots drawn 20 years back, which supports that genetic and epigenetic divergence between subpopulations was caused by artificial disturbance. There was significant fine-scale spatial structuring of MSAP markers in both subpopulations, which in the Undisturbed one was indistinguishable from that of AFLP markers. Genetic differences between subpopulations could be explained by divergent selection alone, while the concerted action of divergent selection and disturbance-driven appearance of new methylation variants in the Disturbed subpopulation is proposed to explain epigenetic differences. This study provides the first empirical evidence to date suggesting that relatively mild disturbances could leave genetic and epigenetic signatures on the next adult generation of long-lived plants., Peer reviewed

Proyecto: //

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/281362
Dataset. 2017

DATA FROM: THE DOMINANT DETRITUS-FEEDING INVERTEBRATE IN ARCTIC PEAT SOILS DERIVES ITS ESSENTIAL AMINO ACIDS FROM GUT SYMBIONTS

  • Larsen, Thomas
  • Ventura, Marc
  • Maraldo, Kristine
  • Triadó-Margarit, Xavier
  • Casamayor, Emilio O.
  • Wang, Yiming V.
  • Andersen, Nils
  • O'Brien, Diane M.
13C values of amino acids Sample 13C values (‰) of amino acids. Each sample was analyzed in triplicate (mean ± SD). NA indicates missing values or replicates. The values are compiled from this study and Larsen et al. (Plos One 2013) (marked with asterisk under sample code). Larsen_13CAA.csv, Supplementation of nutrients by symbionts enables consumers to thrive on resources that might otherwise be insufficient to meet nutritional demands. Such nutritional subsidies by intracellular symbionts has been well studied; however, supplementation of de novo synthesized nutrients to hosts by extracellular gut symbionts is poorly documented, especially for generalists with relatively undifferentiated intestinal tracts. Although gut symbionts facilitate degradation of resources that would otherwise remain inaccessible to the host, such digestive actions alone cannot make up for dietary insufficiencies of macronutrients such as essential amino acids (EAA). Documenting whether gut symbionts also function as partners for symbiotic EAA supplementation is important because the question of how some detritivores are able to subsist on nutritionally insufficient diets has remained unresolved. To answer this poorly-understood nutritional aspect of symbiont-host interactions, we studied the enchytraeid worm, a bulk soil feeder that thrives in arctic peatlands. In a combined field and laboratory study, we employed stable isotope fingerprinting of amino acids to identify the biosynthetic origins of amino acids to bacteria, fungi and plants in enchytraeids. Enchytraeids collected from arctic peatlands derived more than 80% of their EAA from bacteria. In a controlled feeding study with the enchytraeid Enchytraeus crypticus, EAA derived almost exclusively from gut bacteria when the worms fed on higher fiber diets, whereas most of the enchytraeids' EAA derived from dietary sources when fed on lower fiber diets. Our gene sequencing results of gut microbiota showed that the worms harbor several taxa in their gut lumen absent from their diets and substrates. Almost all gut taxa are candidates for EAA supplementation because almost all belong to clades capable of biosynthesizing EAA. Our study provides the first evidence of extensive symbiotic supplementation of EAA by microbial gut symbionts, and demonstrate that symbiotic bacteria in the gut lumen appear to function as partners for both symbiotic EAA supplementation as well as for digestion of insoluble plant fibers., Peer reviewed

Proyecto: //

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/281373
Dataset. 2017

DATA FROM: DEVELOPMENTAL MECHANISMS OF STRIPE PATTERNS IN RODENTS

  • Mallarino, Ricardo
  • Henegar, Corneliu
  • Mirasierra, Mercedes
  • Manceau, Marie
  • Schradin, Carsten
  • Vallejo, Mario
  • Beronja, Slobodan
  • Barsh, Gregory S.
  • Hoekstra, Hopi E.
De novo assembly of Rhabdomys pumilio transcriptome and detailed differential gene expression results This dataset contains a fasta file, including the de novo assembly of Rhabdomys pumilio transcriptome (built from RNA-Seq data using the Trinity suite of de novo transcriptome assembly tools - https://github.com/trinityrnaseq/trinityrnaseq/wiki), as well as a tab separated text file indicating the gene annotations corresponding to each assembly contig, obtained by mapping the de novo assembly to the human reference transcriptome, as described in the manuscript. A third Excel file contains the detailed results of RNA-Seq differential gene expression study in skin obtained from three dorsal regions, as described in the manuscript. Rhabdomys_pumilio.zip, Mammalian colour patterns are among the most recognizable characteristics found in nature and can have a profound impact on fitness. However, little is known about the mechanisms underlying the formation and subsequent evolution of these patterns. Here we show that, in the African striped mouse (Rhabdomys pumilio), periodic dorsal stripes result from underlying differences in melanocyte maturation, which give rise to spatial variation in hair colour. We identify the transcription factor ALX3 as a regulator of this process. In embryonic dorsal skin, patterned expression of Alx3 precedes pigment stripes and acts to directly repress Mitf, a master regulator of melanocyte differentiation, thereby giving rise to light-coloured hair. Moreover, Alx3 is upregulated in the light stripes of chipmunks, which have independently evolved a similar dorsal pattern. Our results show a previously undescribed mechanism for modulating spatial variation in hair colour and provide insights into how phenotypic novelty evolves., Peer reviewed

Proyecto: //

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/281381
Dataset. 2017

DATA FROM: SPATIO-TEMPORAL MONITORING OF DEEP-SEA COMMUNITIES USING METABARCODING OF SEDIMENT DNA AND RNA

  • Guardiola, Magdalena
  • Wangensteen, Owen S.
  • Taberlet, Pierre
  • Coissac, Eric
  • Uriz, María Jesús
  • Turon, Xavier
MOTUdataset This excel file presents filtered data found in the samples with the 18S_allshorts primers. It contains the number of occurrences of each MOTU in the different samples, as well as different columns related to the taxonomic identification of the MOTUs and the representative sequences of them assembled sequences Fasta file containing the raw assembled sequences (singletons eliminated). For each sequence the codes of the samples where the sequence has been found are indicated, as well as the number of reads in each, the total counts of the sequence, and the sequence length assembled seqs.fasta code equivalences This excel file provides the equivalences of sample codes between the file MOTUdataset.xlsx and the file assembled seqs.fasta codes.xlsx, We assessed spatio-temporal patterns of diversity in deep-sea sediment communities using metabarcoding. We chose a recently developed eukaryotic marker based on the v7 region of the 18S rRNA gene. Our study was performed in a submarine canyon and its adjacent slope in the Northwestern Mediterranean Sea, sampled along a depth gradient at two different seasons. We found a total of 5,569 molecular operational taxonomic units (MOTUs), dominated by Metazoa, Alveolata and Rhizaria. Among metazoans, Nematoda, Arthropoda and Annelida were the most diverse. We found a marked heterogeneity at all scales, with important differences between layers of sediment and significant changes in community composition with zone (canyon vs slope), depth, and season. We compared the information obtained from metabarcoding DNA and RNA and found more total MOTUs and more MOTUs per sample with DNA (ca. 20% and 40% increase, respectively). Both datasets showed overall similar spatial trends, but most groups had higher MOTU richness with the DNA template, while others, such as nematodes, were more diverse in the RNA dataset. We provide metabarcoding protocols and guidelines for biomonitoring of these key communities in order to generate information applicable to management efforts., Peer reviewed

Proyecto: //

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/281390
Dataset. 2017

DATA FROM: A NOVEL LOCUS ON CHROMOSOME 1 UNDERLIES THE EVOLUTION OF A MELANIC PLUMAGE POLYMORPHISM IN A WILD SONGBIRD

  • Bourgeois, Yann X. C.
  • Delahaie, Boris
  • Gautier, Mathieu
  • Lhuillier, Emeline
  • Malé, Pierre-Jean G.
  • Bertrand, Joris A. M.
  • Cornuault, Josselin
  • Wakamatsu, Kazumasa
  • Bouchez, Olivier
  • Mould, Claire
  • Bruxaux, Jade
  • Holota, Hélène
  • Milá, Borja
  • Thébaud, Christophe
Data for microsatellites, spectrophotometry and melanins dosage This file contains 1) microsatellites data in GENPOP format, 2) morphometric data for birds included in the pedigree analysis, 3) Spectrophotometry measurements and 4) Results from the dosage of melanins for 12 individuals. Localities coordinates are also provided. All_data_microsats_spectro_melanindosage.xlsx Unfiltered VCF file for 42 individuals SNPs calls for individual GBS data from the same three localities as in Pooled RAD-seq. Colors and localities for each individuals are provided in a separate excel file. Highlands_dataset.recode.vcf Information about individuals included in the GBS VCF file Morph and localities for each individual included in the GBS study. Localities_Morphs_individuals_VCF.xlsx, Understanding the mechanisms responsible for phenotypic diversification within and among species ultimately rests with linking naturally occurring mutations to functionally and ecologically significant traits. Colour polymorphisms are of great interest in this context because discrete colour patterns within a population are often controlled by just a few genes in a common environment. We investigated how and why phenotypic diversity arose and persists in the Zosterops borbonicus white-eye of Reunion (Mascarene archipelago), a colour polymorphic songbird in which all highland populations contain individuals belonging to either a brown or a grey plumage morph. Using extensive phenotypic and genomic data, we demonstrate that this melanin-based colour polymorphism is controlled by a single locus on chromosome 1 with two large-effect alleles, which was not previously described as affecting hair or feather colour. Differences between colour morphs appear to rely upon complex cis-regulatory variation that either prevents the synthesis of pheomelanin in grey feathers, or increases its production in brown ones. We used coalescent analyses to show that, from a ‘brown’ ancestral population, the dominant ‘grey’ allele spread quickly once it arose from a new mutation. Since colour morphs are always found in mixture, this implies that the selected allele does not go to fixation, but instead reaches an intermediate frequency, as would be expected under balancing selection., Peer reviewed

Proyecto: //

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