Resultados totales (Incluyendo duplicados): 5
Encontrada(s) 1 página(s)
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/119378
Otros (other). 2018

EFFECT OF SEX ON GLUCOSE HANDLING BY ADIPOCYTES ISOLATED FROM RAT SUBCUTANEOUS, MESENTERIC AND PERIGONADAL ADIPOSE TISSUE (RAW DATA)

  • Rotondo, Floriana
  • Ho-Palma, Ana Cecilia
  • Remesar Betlloch, Xavier
  • Fernández López, José Antonio
  • Romero Romero, María del Mar
  • Alemany, Marià, 1946-
Dades primàries associades a un article publicat a la revista PeerJ https://doi.org/10.7717/peerj.5440

Proyecto: //
DOI: http://hdl.handle.net/2445/119378
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/119378
HANDLE: http://hdl.handle.net/2445/119378
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/119378
PMID: http://hdl.handle.net/2445/119378
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/119378
Ver en: http://hdl.handle.net/2445/119378
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/119378

Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/66010
Dataset. 2015

INFLUENCE OF A HYPERLIPIDIC DIET ON THE COMPOSITION OF THE NON-MEMBRANE LIPID 6 POOL OF RED BLOOD CELLS OF MALE AND FEMALE RATS (RAW DATA)

  • Remesar Betlloch, Xavier
  • Antelo, Arantxa
  • Llivina, Clàudia
  • Albà, Emma
  • Berdié Rabanaque, Lourdes
  • Agnelli, Silvia
  • Arriarán, Sofía
  • Fernández López, José Antonio
  • Alemany, Marià, 1946-
Dades primàries associades a l'article publicat a la revista PeerJ disponible a l'adreça http://dx.doi.org/10.7717/peerj.1083, Podeu consultar l'article associat a: http://hdl.handle.net/2445/66497, El material que es diposita correspon a les dades experimentals, i part del procés de tabulació d'aquestes, d'un estudi de distribució d'àcids grassos en el lípids d'un nou compartiment lipídic dels glòbuls vermells de rata.

Proyecto: //
DOI: http://hdl.handle.net/2445/66010
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/66010
HANDLE: http://hdl.handle.net/2445/66010
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/66010
PMID: http://hdl.handle.net/2445/66010
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/66010
Ver en: http://hdl.handle.net/2445/66010
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/66010

Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/96861
Dataset. 2016

CAFETERIA DIET MODIFIES BLOOD FLOW DISTRIBUTION OF MALE RATS (RAW DATA)

  • Sabater Martínez, David
  • Agnelli, Silvia
  • Arriarán, Sofía
  • Romero Romero, María del Mar
  • Fernández López, José Antonio
  • Alemany, Marià, 1946-
  • Remesar Betlloch, Xavier
Dades primàries associades a un article enviat a la revista PeerJ i pendent d'avaluació (Abril 2016), Podeu consultar l'article a: http://hdl.handle.net/2445/101599, Dades experimentals de la recerca on s'ha provat de veure si l'administració d'una dieta hiperlipídics (dieta de cafeteria) a rates mascles durant 30 dies, modifica la distribució del fluxs de sang entre els diferents teixits. Això s'ha fet mitjançant l'administració de microesferes fluorescents. S'ha pogut comprobar que hi ha un augment de flux cap el teixit adipós marró, pulmons i cor i una disminució cap a la pell. Això confirma l'augmenten en els teixits que controlen l'energia disipada.

Proyecto: //
DOI: http://hdl.handle.net/2445/96861
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/96861
HANDLE: http://hdl.handle.net/2445/96861
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/96861
PMID: http://hdl.handle.net/2445/96861
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/96861
Ver en: http://hdl.handle.net/2445/96861
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/96861

Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/102243
Dataset. 2016

QUANTITATIVE ANALYSIS OF RAT ADIPOSE TISSUE CELL RECOVERY, AND NON-FAT CELL VOLUME, IN PRIMARY CELL CULTURES (RAW DATA)

  • Rotondo, Floriana
  • Romero Romero, María del Mar
  • Ho-Palma, Ana Cecilia
  • Remesar Betlloch, Xavier
  • Fernández López, José Antonio
  • Alemany, Marià, 1946-
Dades primàries associades a un article publicat a la revista PeerJ disponible a l'adreça http://dx.doi.org/10.7717/peerj.2725, Podeu consultar l'article a: http://hdl.handle.net/2445/104686, Background. White adipose tissue (WAT) is a complex, diffuse, multifunctional organ which contains adipocytes, and a large proportion of fat, but also other cell types, active in defence, regeneration and signalling functions. Studies with adipocytes often require their isolation from WAT by breaking up the matrix of collagen fibres, however, it is unclear to what extent adipocyte number in primary cultures correlates with their number in intact WAT, since recovery and viability are often unknown. Experimental design. Epididymal WAT of 4-6 young adult rats was used to isolate adipocytes with collagenase. Careful recording of lipid content of tissue, and all fraction volumes and weights, allowed us to trace the amount of initial WAT fat remaining in the cell preparation. Functionality was estimated by incubation with glucose and measurement of lactate, glycerol and NEFA production. Non-adipocyte cells were also recovered and their sizes (and those of adipocytes) were also measured. The presence of non-nucleated cells (erythrocytes) was also estimated. Results. Cell numbers and sizes were correlated from all fractions to intact WAT. Tracing the lipid content, the recovery of adipocytes in the final, metabolically active, preparation was in the range of 70-75%. Adipocytes were 7%, erythrocytes 68% and other stromal (nucleated cells) 24% of total WAT cells. However, their overall volumes were, 91%, 0.05%, and 0.2% of WAT. Non-fat volume of adipocytes was 2.5% of WAT. Conclusions. The methodology presented here allows for a direct quantitative reference to the original tissue of studies using isolated cells. We have found, also, that the "live cell mass" of adipose tissue is very small (about 25 µL/g for adipocytes and 2 µL/g stromal, plus about 1 µL/g blood). This fact, translates (with respect to the actual "live cytoplasm" size) into an extremely high metabolic activity, which make WAT an even more significant agent in the control of energy metabolism.

Proyecto: //
DOI: http://hdl.handle.net/2445/102243
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/102243
HANDLE: http://hdl.handle.net/2445/102243
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/102243
PMID: http://hdl.handle.net/2445/102243
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/102243
Ver en: http://hdl.handle.net/2445/102243
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/102243

Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/111074
Dataset. 2017

IN RATS FED HIGH-ENERGY DIETS, TASTE –RATHER THAN FAT CONTENT– IS THE KEY FACTOR INCREASING FOOD INTAKE. COMPARISON OF A CAFETERIA AND A LIPID-SUPPLEMENTED STANDARD DIET (RAW DATA)

  • Oliva Lorenzo, Laia
  • Aranda, Tània
  • Caviola, Giada
  • Fernández-Bernal, Anna
  • Alemany, Marià, 1946-
  • Fernández López, José Antonio
  • Remesar Betlloch, Xavier
Dades primàries associades a un article enviat a la revista PeerJ i pendent d'avaluació (maig 2017), Podeu consultar l'article a: http://hdl.handle.net/2445/115482, Background: Diet deeply affects the food selection and ingestion both in humans and rodents, often resulting in excess energy intake. Methods: We investigated this process comparing two different high-fat dietary approaches to induce obesity, in which all rats received about 40% of their energy intake as lipids. The main nutrient difference between the diets, when compared with controls fed standard lab chow, was the lipid content. Cafeteria diets (K) were devised to be tasty, and thus highly desirable to the rats, mainly for its diverse mix of tastes, particularly salty and sweet. This diet was compared with another high-fat (HF) potentially obesogenic diet, devised not to be as tasty as K, and prepared just supplementing standard chow pellets with fat. We also analysed the influence of sex on the effects of the diets. Results: K rats grew faster, especially the males, although females showed a higher proportion of body lipid, because of a high lipid, sugar and protein intake. HF weight change rates were not different from those of controls. In addition to high sugar, K rats also ingested large amounts of salt. With this study we have shown that the key factor eliciting the excess energy intake in a high-energy diet rat model was not solely or mainly their fat intake. The changes in body fat accrual were more a consequence of their appetence for the food. Conclusions: The results show that the significant presence of sugar and salt is a powerful factor promoting excess food intake, more effective than increasing diet lipid content. These effects were already observed after a relatively short treatment, additionally confirming the differential effects of sex on the hedonic and obesogenic response to diet.

Proyecto: //
DOI: http://hdl.handle.net/2445/111074
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/111074
HANDLE: http://hdl.handle.net/2445/111074
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/111074
PMID: http://hdl.handle.net/2445/111074
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/111074
Ver en: http://hdl.handle.net/2445/111074
Dipòsit Digital de la UB
oai:diposit.ub.edu:2445/111074

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