Resultados totales (Incluyendo duplicados): 18
Encontrada(s) 2 página(s)
Encontrada(s) 2 página(s)
CORA.Repositori de Dades de Recerca
doi:10.34810/data592
Dataset. 2023
DEMOGRAPHIC SURVEY
- Paula Ruiz-Castillo
- Saimado Imputiua
- Kexin Xie
- Eldo Elobolobo
- Patricia Nicolas
- Julia Montana
- Edgar Jamisse
- Humberto Munguambe
- Felisbela Materrula
- Aina Casellas
- Xinwei Deng
- Achla Marathe
- Regina Rabinovich
- Francisco Saute
- Carlos Chaccour
- Charfudin Sacoor
Data corresponding to a demographic survey conducted in Mopeia district (Zambezia province, Mozambique) to inform the Broad One Health Endectocide-based Malaria Intervention in Africa project, a cluster randomized clinical trial. Data consists of household and individual level variables.
Proyecto: //
CORA.Repositori de Dades de Recerca
doi:10.34810/data682
Dataset. 2023
WEALTH INDEX DATA
- Achla Marathe
- Aina Casellas
- Carlos Chaccour
- Cassidy Rist
- Charfudin Sacoor
- Edgar Jamisse
- Eldo Elobolobo
- Felisbela Materrula
- Francisco Saute
- Humberto Munguambe
- Julia Montana
- Kexin Xie
- Mussa Sale
- Patricia Nicolas
- Paula Ruiz-Castillo
- Regina Rabinovich
- Saimado Imputiua
- Victor Mutepa
- Xinwei Deng
Data corresponding to household socioeconomic characteristics collected in a demographic survey conducted in Mopeia district (Zambezia province, Mozambique).
Proyecto: //
CORA.Repositori de Dades de Recerca
doi:10.34810/data745
Dataset. 2023
SNAKEBITE DATA
- Aina Casellas
- Carlos Chaccour
- Charfudin Sacoor
- Edgar Jamisse
- Eldo Elobolobo
- Emma O'Bryan
- Francisco Saute
- Humberto Munguambe
- Julia Montana
- Patricia Nicolas
- Paula Ruiz-Castillo
- Regina Rabinovich
- Saimado Imputiua
Data corresponding to snakebite characteristics collected in a demographic survey conducted in Mopeia district (Zambezia province, Mozambique).
Proyecto: //
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351957
Dataset. 2023
PBIO.3002315.T002 - LINEAGE TRACING IDENTIFIES HETEROGENEOUS HEPATOBLAST CONTRIBUTION TO CELL LINEAGES AND POSTEMBRYONIC ORGAN GROWTH DYNAMICS [DATASET]
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
pbio.3002315.t002 -
Lineage tracing identifies heterogeneous hepatoblast contribution to cell lineages and postembryonic organ growth dynamics, Peer reviewed
Proyecto: //
DOI: http://hdl.handle.net/10261/351957
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351957
HANDLE: http://hdl.handle.net/10261/351957
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351957
PMID: http://hdl.handle.net/10261/351957
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351957
Ver en: http://hdl.handle.net/10261/351957
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351957
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351973
Dataset. 2023
LINEAGE TRACING REVEALS HETEROGENEOUS CLUSTER TOPOLOGIES DURING POSTEMBRYONIC GROWTH [DATASET]
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
(A) Schematic depicting key stages in postembryonic zebrafish liver development. (B) Experimental schematics of long-term lineage tracing experiments using fraeppli-nls embryos, inducing recombination by heat shock at 26 hpf to label hepatoblasts. At 120 hpf, embryos were screened by live imaging at the confocal microscope, and only sparsely labelled embryos were raised and fixed in either juvenile or adult stages. (C-H) Recombined livers showed different cluster topologies: clusters along central veins (C-C’) (n = 9 livers), proximal–distal stripes (D) (n = 23 livers) or giant clusters in the ventral lobe in adult (F-G’) (n = 3 livers). Large clusters in the ventral lobe can originate from one single-labelled cell at 5 dpf (n = 1 liver) (E). (F) Stereomicroscope image showing the spatial location of the giant clone originating from a single recombined cell (H). Recombined livers show a range of cluster sizes from small (H’) to medium (H”). (I) Schematics of characteristic cluster topologies in recombined livers. Red lines indicate the blood vessel orientation in the liver. (C-H) Total numbers are (N = 9, n = 79 livers). A, anterior; P, posterior; R, right; L, left; RL, right lobe; LL, left lobe; VL, ventral lobe., Peer reviewed
Proyecto: //
DOI: http://hdl.handle.net/10261/351973
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351973
HANDLE: http://hdl.handle.net/10261/351973
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351973
PMID: http://hdl.handle.net/10261/351973
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351973
Ver en: http://hdl.handle.net/10261/351973
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351973
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351980
Dataset. 2023
QUANTITATIVE LINEAGE TRACING OF HEPATOBLASTS DURING EMBRYONIC DEVELOPMENT IDENTIFIES HETEROGENEOUS GROWTH BEHAVIOUR [DATASET]
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
(A) Frequency of manually assigned pure hepatocyte clone sizes (N = 6, n = 190 clones). (B) Distribution of the corresponding number of cell divisions for each pure hepatocyte clone (N = 6, n = 190 clones). (A, B) Clone colours are plotted in blue (TagBFP), turquoise (mTFP1), magenta (mKate2), and orange (E2-Orange); the mean of all colours is represented in black. (C) Whole-mount of a 100-hpf liver showing several clones, including a mKate2+ 1-cell clone (N = 6, n = 15 livers). (D) Liver with a medium size 12-cell mTFP1+ clone (N = 6, n = 7 livers). (E) Whole-mount of a 100-hpf liver with a large 33-cell TagBFP+ clone (N = 1, n = 1 livers). (C-E) Labelled cells are represented as segmented nuclei, and an overall segmentation of the whole liver tissue is shown in transparent grey. The numerical values that were used to generate the graphs in (A, B) can be found in S1 Data., Peer reviewed
Proyecto: //
DOI: http://hdl.handle.net/10261/351980
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351980
HANDLE: http://hdl.handle.net/10261/351980
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351980
PMID: http://hdl.handle.net/10261/351980
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351980
Ver en: http://hdl.handle.net/10261/351980
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351980
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351993
Dataset. 2023
QUANTITATIVE LINEAGE TRACING IDENTIFIES UNI- AND BIPOTENT HEPATOBLAST CONTRIBUTIONS DURING LINEAGE DECISIONS [DATASET]
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
(A) Schematic of FRaeppli-NLS cassette including attB and attP sites for PhiC31-mediated recombination and the 4 FRaeppli FPs: TagBFP, mTFP1, mKate2, and E2-Orange. Recombination is induced by combining fraeppli-nls with hsp70l:phiC31; prox1a:kalTA4; see S3A Fig. (B) Key steps of liver development in zebrafish: After hepatoblast specification, the differentiation into BECs and hepatocytes is initiated at around 42 hpf. Differentiated cells acquire polarity and form a functional architecture by 120 hpf. (C) Experimental strategy for tracing progeny of individual hepatoblasts using fraeppli-nls: Heat shock at 26 hpf controls PhiC31 expression followed by attB-attP recombination. Embryos were fixed at 100 hpf for analysis. (D-F) Whole-mount livers at 100 hpf showing (D) mixed clone composed of hepatocytes and BECs (D’) (N = 6, n = 23 clones); (E) clones formed by pure hepatocytes (E’-E”) (N = 6, n = 190 clones); and (F) example of pure BEC clone coexpressing TagBFP and mTFP1 (white, coexpressing cells were manually segmented and masked). (F’) (N = 2, n = 2 clones). (D-F) An overall segmentation of the whole liver tissue is shown in transparent grey. (G) Pie charts showing the total number of labelled embryos and clones with manually assigned lineage contributions (N = 6, n = 214 clones; in 2 of the 6 experiments, nuclear shape indicated BEC fate). The numerical values that were used to generate the graphs in (G) can be found in S1 Data. BEC, biliary epithelial cell; FP, fluorescent protein; hpf, hours post fertilization., Peer reviewed
Proyecto: //
DOI: http://hdl.handle.net/10261/351993
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351993
HANDLE: http://hdl.handle.net/10261/351993
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351993
PMID: http://hdl.handle.net/10261/351993
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351993
Ver en: http://hdl.handle.net/10261/351993
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/351993
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005
Dataset. 2023
HEPATIC PROLIFERATION DYNAMICS AND EARLY ESTABLISHMENT OF A 1:9 BEC:HEPATOCYTE RATIO DURING EMBRYONIC DEVELOPMENT [DATASET]
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
(A) Approximately 5 μm projection of a 72-hpf liver expressing tp1:H2B-mCherry (BEC), stained for Hnf4a (hepatocytes) and EdU (proliferating cells). Yellow and white arrowheads highlight proliferating BECs and hepatocytes, respectively (N = 2, n = 10 livers). (B) Graph showing the proportion of EdU+ proliferating hepatocytes and BECs over time (N = 2, n ≥ 8). (C, D) Graph showing hepatocyte (C) and BEC (D) cell numbers during development (N = 4, n ≥ 12 livers). (E) Quantification of total liver volume during development determined in embryos in BABB (N = 4, n ≥ 12 livers). (F) Maximum projection (20 μm z-stacks) of a 48-hpf liver expressing tp1:H2B-mCherry (BEC) and stained for Hnf4ɑ (hepatocyte). (G) Relative distribution of BECs and hepatocytes during development from 48 to 144 hpf (N = 4, n ≥ 12 livers). (B-E) Different shape data points indicate different experiments. The numerical values that were used to generate the graphs in (B-E, G) can be found in S1 Data. BEC, biliary epithelial cell; EdU, 5-ethynyl-2′-deoxyuridine; hpf, hours post fertilization., Peer reviewed
Proyecto: //
DOI: http://hdl.handle.net/10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005
HANDLE: http://hdl.handle.net/10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005
PMID: http://hdl.handle.net/10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005
Ver en: http://hdl.handle.net/10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352005
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352014
Dataset. 2023
ESTABLISHMENT OF BEC AND HEPATOCYTE LINEAGES: IN VIVO CELL TYPE QUANTIFICATION AND IN SILICO MODELLING [DATASET]
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
(A) Schematic of a 5-dpf liver, highlighting the biliary network. (B-B’) Maximum projection (200 μm z-stack) of a 120-hpf liver expressing tp1:H2B-mCherry (BEC) and stained for Hnf4ɑ (hepatocyte). Autofluorescent blood cells appear in bright white. (N = 4, n ≥ 12 livers) (C) Relative distribution of BECs and hepatocytes at 120 hpf (N = 4, n ≥ 12 livers). (D-F) Mathematical models simulating hepatoblast differentiation employing different parameter combinations: proliferation rates of differentiated cell types is equal (D, F) or slower in BECs (E). Hepatoblasts either are all bipotent (D, E) or represent a heterogeneous population with mixed probabilities for uni-or bipotent differentiation (F). Plots showing the simulated cell proportions over simulation time (n = 10) and the final cell type ratio in bar graphs. The numerical values that were used to generate the graphs in (C-F) can be found in S1 Data. BEC, biliary epithelial cell; dpf, day postfertilization; Hb, hepatoblast; Hc, hepatocyte; hpf, hours post fertilization., Peer reviewed
Proyecto: //
DOI: http://hdl.handle.net/10261/352014
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352014
HANDLE: http://hdl.handle.net/10261/352014
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352014
PMID: http://hdl.handle.net/10261/352014
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352014
Ver en: http://hdl.handle.net/10261/352014
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352014
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352036
Dataset. 2023
POSTEMBRYONIC VENTRAL LOBE FORMATION [DATASET]
- Unterweger, Iris. A.
- Klepstad, Julie
- Hannezo, Edouard
- Lundegaard, Pia R.
- Trusina, Ala
- Ober, Elke A.
(A, B) Confocal images of the same liver showing the embryonic left liver lobe at 5 dpf with a 3-cell mKate2+ clone (A) and at juvenile stage (SL = 14.4 mm) including a continuous Kate2+ clone in the ventral lobe (N = 1, n = 1 liver). (C, D) Juvenile livers (C–SL = 8.46 mm and D–SL = 10.93 mm) with connected clusters that are oriented along the tissue edge and spread through the left and the ventral lobe. Arrows indicate cluster growth direction (N = 4, n = 14 livers). (E-P) Brightfield images of stages I-VI livers in loco within the fish (E, G, I, K, M, O) or dissected out (F, H, J, L, N, P). In (M), the liver is removed and the gut bend is visible. A, anterior; P, posterior; R, right; L, left; RL, right lobe; LL, left lobe; VL, ventral lobe., Peer reviewed
Proyecto: //
DOI: http://hdl.handle.net/10261/352036
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352036
HANDLE: http://hdl.handle.net/10261/352036
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352036
PMID: http://hdl.handle.net/10261/352036
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352036
Ver en: http://hdl.handle.net/10261/352036
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/352036
Advanced search