BUSCADOR RECOLECTA

The development of three-dimensional environments to mimic the in vivo cellular response is a problem in the building of disease models. This study aimed to synthesize and validate three-dimensional support for culturing monoclonal plasma cells (mPCs) as a disease model for multiple myeloma. The three-dimensional environment is a biomimetic microgel formed by alginate microspheres and produced on a microfluidic device whose surface has been functionalized by a layer-by-layer process with components of the bone marrow’s extracellular matrix, which will interact with mPC. As a proof of concept, RPMI 8226 cell line cells were cultured in our 3D culture platform. We proved that hyaluronic acid significantly increased cell proliferation and corroborated its role in inducing resistance to dexamethasone. Despite collagen type I having no effect on proliferation, it generated significant resistance to dexamethasone. Additionally, it was evidenced that both biomolecules were unable to induce resistance to bortezomib. These results validate the functionalized microgels as a 3D culture system that emulates the interaction between tumoral cells and the bone marrow extracellular matrix. This 3D environment could be a valuable culture system to test antitumoral drugs efficiency in multiple myeloma.

[EN] This study examines the degradation of atrazine (ATZ) with Pt-modified textile electrodes using an electrochemical method that is comparatively studied in two electrochemical cell configurations: cells with separated anodic and cathodic compartments (divided configuration); and without any separation (undivided configuration). The influence of the presence of chloride ions was studied. The best results were obtained when an undivided cell was used. The morphology and composition of the dispersed Pt coatings were analyzed using field emission scanning electron microscopy (FESEM) and Energy Dispersive X-Ray Analysis. The FESEM analyses confirmed that the textile surface was effectively modified by the electrocatalytic material. High performance liquid chromatography, gas chromatography mass spectrometry, and spectroscopic methods were used to follow the evolution of major oxidation products. Total organic carbon, chemical oxygen demand, and total nitrogen were used to evaluate the degradation efficiency of treated aqueous solutions. The experimental results obtained indicate that the efficiency of the electrochemical treatment was high with a low energy consumption when using electrodes based on textile materials, such as anodes or as cathodes (in particular, in electrolysis without compartment separation). All these can be produced at very competitive prices., Chemviron Carbon who kindly donated the FlexzorbTM FM10 activated carbon fabrics and Funding for open access charge, CRUE-Universitat Politècnica de València, are acknowledged.