BUSCADOR RECOLECTA

Experimental Procedures for Supplemental Figure: Double-stranded Zic2 and Zic2 mutant decoy DNA were prepared by annealing complementary single strands with the follow sequences: 5′-TCTTGGGTGGTCTCCGGAGACCACCCAAGA (for Zic2 decoy) and 5′-TCTTGAGTGAACTCCGGAGTTCACTCAAGA (for Zic2M decoy) as a negative control (Mizugishi et al., 2001). We utilized unlabeled and also Texas-red-tagged oligonucleotides (Invitrogen) to monitor DNA entry into cells. The terminal four bases on either site of the molecule were linked by phosphorothioate esters for added stability. Retinal explants were exposed to serial concentrations (0, 10, 100, and 200 μM) of decoy DNA combined with serial concentrations of serum-free medium (SFM) plus Fungene 6 transfection reagent (Roche) and incubated for 1 hr 30 min at 37°C. The explants were then washed several times in SFM, plated on D-polylysine/laminin, and incubated at 37°C, and dissociated chiasm cells were added to the cultures 2 hr later. They were then immunolabeled with α-neurofilament and explant outgrowth quantified, as indicated in Experimental Procedures., Figure S1. Zic2 Is Necessary for Ventrotemproal (VT) RGCs to Respond Negatively to Cues from the Chiasm Midline In Vitro. (A) Retinal explants grown on dissociated chiasm cells and immunostained with α-neurofilament. Representative images of dorsotemporal (DT) (a–d) and VT (e–h) retinal explants that were untransfected (a and e) or incubated with lipofectamine (b and f), Zic2 decoy oligos (c and g), or Zic2-mutant-decoy oligos (d and h) and subsequently cocultured with dissociated chiasm cells. Scale bar: 100 μm. (B) Quantification of area occupied by axons of DT (black columns) or VT (white columns) retinal explants that were untransfected or incubated with lipofectamine, Zic2-decoy oligos, or mutant-Zic2-decoy oligos, and then cocultured with dissociated chiasm cells. Left panels and graph: transfection of Zic2-decoy oligos does not affect the outgrowth in DT neurites (p > 0.2). Right panels and graph: transfection of Zic2-decoy oligos leads to increased growth of VT neurites. (*p < 0.05 compared with VT explants incubated with the lipofection agent alone or transfected with Zic2M-decoy). Number above bars indicates number of explants., Peer reviewed

Preparation procedures and physical and spectroscopic data for compounds 17−43 and crystallographic data in CIF format and ORTEP diagrams of 28 and 30., Peer reviewed

Analytical and spectral characterization data of all new compounds, NMR/NOESY interprotonic distances, and crystallographic data (CIF)., Peer reviewed

[CAT] Dades antracològiques procedents de l'excavació d'urgència de Can Viola, realitzat l'any 2003 en el context de l'obra d'ampliació de la variant C-59. Excavació d'urgència dirigida per Arcos, R. i Velasco, C. Les dades adjuntades provenen del registre antracològic trobat a l'interior d'un forn datat al Bronce Final (900-650 aC) en la unitat estratigrafia UE-184.

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, [ENG] Anthracological data from the rescue excavation at Can Viola, carried out in 2003 in the context of the expansion works of the C-59 highway. Emergency excavation directed by Arcos, R. and Velasco, C. The attached data come from the anthracological record found inside a furnace dated to the Late Bronze Age (900-650 BC) in the UE-184 stratigraphic unit.

Anthracological data from the rescue excavations at Catillo de Olmos (Toledo). The attached data come from the anthracological and carpological records from an islamic fortress.

Dades d'ús de prèstec del CRAI de la UB de l'any 2002. Inclou en format csv les dades mensuals i el recull anual. Dades desglossades per: tipus d'operació, tipus de lector, per tipus de material, llengua i any de publicació. Les dades corresponents al mes de maig no estan disponibles

Dades d'ús de prèstec del CRAI de la UB de l'any 2003. Inclou en format csv les dades mensuals i el recull anual. Dades desglossades per: tipus d'operació, tipus de lector, per tipus de material, per llengua i any de publicació. Les dades corresponents al mes de novembre no estan disponibles

Dades relatives al nombre de cerques al catàleg de fons modern del CRAI de la UB durant el curs 2002-2003. Inclou en format csv les dades mensuals desglossades per tipus de cerca i per la ubicació des d'on s'ha fet la consulta.

Dades relatives al nombre de cerques al catàleg de fons modern del CRAI de la UB durant el curs 2003-2004. Inclou en format csv les dades mensuals desglossades per tipus de cerca i per la ubicació des d'on s'ha fet la consulta.

Peer reviewed, Fecha 2014-09-02.--Fujitsu ScanSnap iX500.--Unidad técnica IHMC.--Archivo depósito IHMC.--Solo se permite el uso con fines educativos y de investigación