BUSCADOR RECOLECTA

We have previously shown that an HIV vaccine regimen including three doses of HIV-modified vaccinia virus Ankara vector expressing HIV-1 antigens from clade B (MVA-B) was safe and elicited moderate and durable (1 year) T-cell and antibody responses in 75% and 95% of HIV-negative volunteers (n = 24), respectively (RISVAC02 study). Here, we describe the long-term durability of vaccine-induced responses and the safety and immunogenicity of an additional MVA-B boost., Peer reviewed

En este episodio hablamos de uno de los primeros proyectos de Obsevadores del Mar: Crustáceos Decápodos. Sandra Espeja conversa sobre estos animales con David Díaz, investigador en el IEO y coordinador científico del proyecto; Lluc García, naturalista y cofundador del Museo Balear de Ciencias Naturales; e Iván Ramos, jefe del Servicio de Protección de Especies de la Conselleria de Medi ambient i Territori de Baleares.

The deep-water sedimentary processes and morphological features offshore Madeira Island, located in the Central-NE Atlantic have been scantly studied. The analysis of new multibeam bathymetry, echo-sounder profiles and few multichannel seismic reflection profiles allowed us to identify the main geomorphologies, geomorphic processes and their interplay. Several types of features were identified below 3800 m water depth, shaped mainly by i) the interplay between northward-flowing Antarctic Bottom Water (AABW) and turbidity currents and ii) interaction of the AABW with oceanic reliefs and the Madeira lower slope. Subordinate and localized geomorphic processes consist of tectono-magmatic, slope instability, turbidity currents and fluid migration. The distribution of the morphological features defines three regional geomorphological sectors. Sector 1 represents a deep seafloor with its abyssal hills, basement highs and seamounts inherited from Early Cretaceous seafloor spreading. Sector 2 is exclusively shaped by turbidity current flows that formed channels and associated levees. Sector 3 presents a more complex morphology dominated by widespread depositional and erosional features formed by AABW circulation, and localized mixed contourite system developed by the interplay between the AABW circulation and WNW-ESE-flowing turbidite currents. The interaction of the AABW with abyssal hills, seamounts and basement ridges leads to the formation of several types of contourites: patch drifts, double-crest mounded bodies, and elongated, mounded and separated drifts. The patch drifts formed downstream of abyssal hills defining an previously unknown field of relatively small contourites. We suggest they may be a result of localized vortexes that formed when the AABW’s flow impinges these oceanic reliefs producingthe erosional scours that bound these features. The bottom currents in the area are known to be too weak (1–2 cm s− 1) to produce the patch drifts and scours. Therefore, we suggest that these features could be relics at present, having developed when the AABW was stronger than today, as during glacial/end of glacial stages., SUBVENT

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

Presentación oral en el congreso internacional: 14th INTERNATIONAL CORAL REEF SYMPOSIUM, celebrado en Bremen (Alemania) del 19-23 de julio de 2021 (ICRS2021), DETECCIÓN INNOVADORA DE PROLIFERACIONES ALGALES TÓXICAS: UNA NECESIDAD FRENTE AL CALENTAMIENTO GLOBAL, DIANAS

Dinophysis acuminata is the major cause of lengthy harvesting bans in shellfish growing areas in European Atlantic waters. In North Western Iberian shelf waters, the growing season of D. acuminata lasts throughout the upwelling season (ca. from March to September). Once environmental conditions trigger initial population growth, abundance fluctuations are coupled to the event-scale dynamics of coastal upwelling-relaxation/downwelling cycles. The “ASIMUTH-Rias” cruise (17–21 June 2013) in the Galician Rias (Pontevedra and Vigo) and adjacent shelf, during a DSP outbreak, aimed to explore small-scale physical processes associated with late spring blooms (> 103 cells L-1) of D. acuminata. The cruise coincided with the initiation of an upwelling pulse following relaxation and deepening of a previously formed thin layer of centric colony-forming diatoms. In this work, a niche (sensu Hutchinson) approach based on an Outlying Mean Index (OMI) analysis was used to describe the realized niche of D. acuminata during transient conditions. The OMI analysis was applied to fine-scale measurements of physical properties, HPLC-derived pigment composition, and phytoplankton functional groups in cross-shelf transects and in a 36-h study at a fixed station. Although having a wide niche breath, the realized niche of D. acuminata (cell maximum 5 ×103 cells L-1 between 3 and 5 m) was characterized by outflowing warmer (15-16 °C) waters with high turbulence (? < 10-6 m2 s-3), high light intensity, and low nitrate concentrations. These results show that during a transient upwelling-initiation scenario, mid-shelf waters provided a more suitable habitat for D. acuminata and the accompanying dinoflagellate populations than waters inside the rías

Maitotoxins (MTXs) are among the most potent toxins known. These toxins are produced by epi-benthic dinoflagellates of the genera Gambierdiscus and Fukuyoa and may play a role in causing the symptoms associated with Ciguatera Fish Poisoning. A recent survey revealed that, of the species tested, the newly described species from the Canary Islands, G. excentricus, is one of the most maitotoxic. The goal of the present study was to characterize MTX-related compounds produced by this species. Initially, lysates of cells from two Canary Island G. excentricus strains VGO791 and VGO792 were partially purified by (i) liquid-liquid partitioning between dichloromethane and aqueous methanol followed by (ii) size-exclusion chromatography. Fractions from chromatographic separation were screened for MTX toxicity using both the neuroblastoma neuro-2a (N2a) cytotoxicity and Ca2+ flux functional assays. Fractions containing MTX activity were analyzed using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) to pinpoint potential MTX analogs. Subsequent non-targeted HRMS analysis permitted the identification of a novel MTX analog, maitotoxin-4 (MTX4, accurate mono-isotopic mass of 3292.4860 Da, as free acid form) in the most toxic fractions. HRMS/MS spectra of MTX4 as well as of MTX are presented. In addition, crude methanolic extracts of five other strains of G. excentricus and 37 other strains representing one Fukuyoa species and ten species, one ribotype and one undetermined strain/species of Gambierdiscus were screened for the presence of MTXs using low resolution tandem mass spectrometry (LRMS/MS). This targeted analysis indicated the original maitotoxin (MTX) was only present in one strain (G. australes S080911_1). Putative maitotoxin-2 (p-MTX2) and maitotoxin-3 (p-MTX3) were identified in several other species, but confirmation was not possible because of the lack of reference material. Maitotoxin-4 was detected in all seven strains of G. excentricus examined, independently of their origin (Brazil, Canary Islands and Caribbean), and not detected in any other species. MTX4 may therefore serve as a biomarker for the highly toxic G. excentricus in the Atlantic area.

Presentación oral en el congreso internacional: 14th INTERNATIONAL CORAL REEF SYMPOSIUM, celebrado en Bremen (Alemania) del 19-23 de julio de 2021 (ICRS2021), DETECCIÓN INNOVADORA DE PROLIFERACIONES ALGALES TÓXICAS: UNA NECESIDAD FRENTE AL CALENTAMIENTO GLOBAL, DIANAS