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Supplementary methods. Table S1. Primers and templates used for PCRs. Table S2. Sequences of PCR primers used in the construction of chimerical viruses. Table S3. List of primers and templates used in PCRs to generate plasmids based on pMDC32. Table S4. Sequences of PCR primers used to construct plasmids based on pMDC32. Fig. S1. Infectivity of chimerical viruses based on plum pox virus. Fig. S2. Infectivity of chimerical viruses based on plum pox virus. Fig. S3. Anti-silencing activity of CaMV P6 proteins., Peer reviewed

Supplemental Figure 1. Sequence analysis of P1-like proteins from several Potyviridae, and in vitro cleavage activity of CVYV P1a. Supplemental Figure 2. HCPro silencing suppressor activity in P1a-containing constructs in the absence of NIa co-expression., Peer reviewed

To respect the intellectual property rights, protect the rights of data authors, expand services of the data center, and evaluate the application potential of data, data users should clearly indicate the source of the data and the author of the data in the research results generated by using the data (including published papers, articles, data products, and unpublished research reports, data products and other results). For re-posting (second or multiple releases) data, the author must also indicate the source of the original data. Example of acknowledgement statement is included below: The data set is provided by National Tibetan Plateau / Third Pole Environment Data Center (http://data.tpdc.ac.cn)., 1. It is challenge to scale-up from simplified proxies to ecosystem functioning since the inherent complexity of natural ecosystems hinders such an approach. One way to address this complexity is to track ecosystem processes through the lens of plant functional traits. Elevational gradients with diverse biotic and abiotic conditions offer ideal settings for inferring functional trait responses to environmental gradients globally. However, most studies have focused on differences in mean trait values among species and little is known on how intraspecific traits vary along wide elevational gradients and how this variability reflects ecosystem productivity., 2. We measured functional traits of the sub-shrub Koenigia mollis (Basionym: Polygonum molle) (a widespread species) in 11 populations along a wide elevational gradient (1515-4216 m) considering from subtropical forest to alpine biomes treeline in the central Himalayas. After measuring different traits (plant height, specific leaf area, leaf area, length of flowering branches, leaf carbon isotope – δ 13C, leaf carbon and leaf nitrogen concentrations), we investigated drivers on changes of these traits and also characterized their relationships with elevation, climate and net primary productivity (NPP)., 3. All trait values decreased with increasing elevation, except for δ 13C that increased upwards. Likewise, most traits showed strong positive relationships with potential evapotranspiration (PET), while δ 13C exhibited a negative relationship. In this context, elevation-dependent water-energy dynamics is the primary driver of trait variations. Further, five key traits (plant height, specific leaf area, leaf carbon, leaf nitrogen and leaf δ 13C) explained 90.45% of variance in NPP., 4. Synthesis. Our study evidences how elevation-dependent climate variations affect ecosystem processes and functions. Intraspecific variability in functional traits is strongly driven by changes in water-energy dynamics, and reflects changes in community productivity over elevation. K. mollis, with one of the widest elevational ranges known to date, could be a model species to infer functional trait responses to environmental gradients globally. This study sheds new insight on how plants modify their basic ecological strategies to cope with changing environments., National Science and Technology Major Project of China: Second National Science and Technology and Research Programme (STEP)（2019QZKK0000）, Peer reviewed

© Author(s) 2023. CC BY 4.0 License. The copyright of individual parts of the supplement might differ from the article licence., Peer reviewed

7953552.zip contiene 6 ficheros xlsx: Sabocos.xlsx; Urdiceto.xlsx; Marbore.xlsx; Acherito.xlsx; Creguena.xlsx; LaSierra.xlsx, Peer reviewed

Over the last years, perennial ice deposits located within caves have awakened interest as places to study microbial communities since they represent unique cryospheric archives of climate change. Since the beginning of the twentieth century, the temperature has gradually increased, and it is estimated that by the end of this century the increase in average temperature could be around 4.0°C. In this context of global warming the ice deposits of the Pyrenean caves are undergoing a significant regression. Among this type of caves, that on the Cotiella Massif in the Southern Pyrenees is one of the southernmost studied in Europe. These types of caves house microbial communities which have so far been barely explored, and therefore their study is necessary. In this work, the microbial communities of the Pyrenean ice cave A294 were identified using metabarcoding techniques. In addition, research work was carried out to analyze how the age and composition of the ice affect the composition of the bacterial and microeukaryotic populations. Finally, the in vivo effect of climate change on the cellular machinery that allow microorganisms to live with increasing temperatures has been studied using proteomic techniques., Peer reviewed

Over the last years, perennial ice deposits located within caves have awakened interest as places to study microbial communities since they represent unique cryospheric archives of climate change. Since the beginning of the twentieth century, the temperature has gradually increased, and it is estimated that by the end of this century the increase in average temperature could be around 4.0°C. In this context of global warming the ice deposits of the Pyrenean caves are undergoing a significant regression. Among this type of caves, that on the Cotiella Massif in the Southern Pyrenees is one of the southernmost studied in Europe. These types of caves house microbial communities which have so far been barely explored, and therefore their study is necessary. In this work, the microbial communities of the Pyrenean ice cave A294 were identified using metabarcoding techniques. In addition, research work was carried out to analyze how the age and composition of the ice affect the composition of the bacterial and microeukaryotic populations. Finally, the in vivo effect of climate change on the cellular machinery that allow microorganisms to live with increasing temperatures has been studied using proteomic techniques., Peer reviewed

Over the last years, perennial ice deposits located within caves have awakened interest as places to study microbial communities since they represent unique cryospheric archives of climate change. Since the beginning of the twentieth century, the temperature has gradually increased, and it is estimated that by the end of this century the increase in average temperature could be around 4.0°C. In this context of global warming the ice deposits of the Pyrenean caves are undergoing a significant regression. Among this type of caves, that on the Cotiella Massif in the Southern Pyrenees is one of the southernmost studied in Europe. These types of caves house microbial communities which have so far been barely explored, and therefore their study is necessary. In this work, the microbial communities of the Pyrenean ice cave A294 were identified using metabarcoding techniques. In addition, research work was carried out to analyze how the age and composition of the ice affect the composition of the bacterial and microeukaryotic populations. Finally, the in vivo effect of climate change on the cellular machinery that allow microorganisms to live with increasing temperatures has been studied using proteomic techniques., Peer reviewed

Over the last years, perennial ice deposits located within caves have awakened interest as places to study microbial communities since they represent unique cryospheric archives of climate change. Since the beginning of the twentieth century, the temperature has gradually increased, and it is estimated that by the end of this century the increase in average temperature could be around 4.0°C. In this context of global warming the ice deposits of the Pyrenean caves are undergoing a significant regression. Among this type of caves, that on the Cotiella Massif in the Southern Pyrenees is one of the southernmost studied in Europe. These types of caves house microbial communities which have so far been barely explored, and therefore their study is necessary. In this work, the microbial communities of the Pyrenean ice cave A294 were identified using metabarcoding techniques. In addition, research work was carried out to analyze how the age and composition of the ice affect the composition of the bacterial and microeukaryotic populations. Finally, the in vivo effect of climate change on the cellular machinery that allow microorganisms to live with increasing temperatures has been studied using proteomic techniques., Peer reviewed

ESM 1 Contrast tests of pathogen species (‘Sp’) and isolate (‘Is’) nested within species (‘df’, degrees of freedom, ‘Res’, residuals): one-way ANOVA when they meet the assumptions (transformed as x0.3 for lesion and IAP and x0.5 for IRP); χ² for binomial variables and KruskalWallis test when assumptions not met. Bold numbers are significant factors (p ≤ 0.05). Variables: Blue cells in phloem (P) and xylem (X); hyphae (H); tyloses (RT); lignin presence (LI); calloses in sieve plates (SP); lesion (length); necrosis in cambium (NC); fibers/vessels (FV), axial (AP) and ray (RP) parenchyma cells altered and axial (IAP) and ray (IRP) cells altered with iodine-potassium iodide.-- ESM 2 Factor loadings from the principal component analysis (PCA) of the variables measured in alder saplings inoculated with different Phytophthora and Halophytophthora species. Key: 'AB, S', Astra blue and Safranine staining; 'CW', Calcofluor white staining.-- ESM 3 Comparison of correlations (Spearman coefficients) between Phytophthora and Halophytophthora species for the variables lesion length, tyloses and hyphae in phloem and xylem and altered fibers/vessels and axial and ray parenchyma cells (with Astra blue ('AB’) and Safranine (‘S’) or Iodide (‘I’) staining) of inoculated alder saplings. Significance levels: *** p ≤ 0.001; **p  ≤  0.01; *p  ≤  0.05. Significant values (p  ≤  0.05) are shown in bold face.-- ESM 4 Phytophthora species isolated from symptomatic alders in riparian forests of Catalonia (NE Spain), This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/., Peer reviewed