BUSCADOR RECOLECTA

We have previously shown that an HIV vaccine regimen including three doses of HIV-modified vaccinia virus Ankara vector expressing HIV-1 antigens from clade B (MVA-B) was safe and elicited moderate and durable (1 year) T-cell and antibody responses in 75% and 95% of HIV-negative volunteers (n = 24), respectively (RISVAC02 study). Here, we describe the long-term durability of vaccine-induced responses and the safety and immunogenicity of an additional MVA-B boost., Peer reviewed

To investigate the safety and immunogenicity of a modified vaccinia virus Ankara vector expressing HIV-1 antigens from clade B (MVA-B), a phase-I, doubled-blind placebo-controlled trial was performed., Peer reviewed

Attenuated poxvirus vectors expressing human immunodeficiency virus type 1 (HIV-1) antigens are considered promising HIV/AIDS vaccine candidates. Here, we describe the nature of T cell immune responses induced in healthy volunteers participating in a phase I clinical trial in Spain after intramuscular administration of three doses of the recombinant MVA-B-expressing monomeric gp120 and the fused Gag-Pol-Nef (GPN) polyprotein of clade B. The majority (92.3%) of the volunteers immunized had a positive specific T cell response at any time postvaccination as detected by gamma interferon (IFN-γ) intracellular cytokine staining (ICS) assay. The CD4(+) T cell responses were predominantly Env directed, whereas the CD8(+) T cell responses were similarly distributed against Env, Gag, and GPN. The proportion of responders after two doses of MVA-B was similar to that obtained after the third dose of MVA-B vaccination, and the responses were sustained (84.6% at week 48). Vaccine-induced CD8(+) T cells to HIV-1 antigens after 1 year were polyfunctional and distributed mainly within the effector memory (TEM) and terminally differentiated effector memory (TEMRA) T cell populations. Antivector T cell responses were mostly induced by CD8(+) T cells, highly polyfunctional, and of TEMRA phenotype. These findings demonstrate that the poxvirus MVA-B vaccine candidate given alone is highly immunogenic, inducing broad, polyfunctional, and long-lasting CD4 and CD8 T cell responses to HIV-1 antigens, with preference for TEM. Thus, on the basis of the immune profile of MVA-B in humans, this immunogen can be considered a promising HIV/AIDS vaccine candidate., Peer reviewed

Audiovisual sobre el grupo artístico 4 Elementes, autores de Postlibros, Peer reviewed

Vídeo conmemorativo, con el personal y la Dirección del IPNA-CSIC, sobre el Día de la Mujer y la Niña en la Ciencia, Peer reviewed

Referencias: Di Vincenzo, Facundo. "El viaje de Elías Castelnuovo a la Unión Soviética en 1932". Rebelión.org (28.09.2021), https://rebelion.org/el-viaje-de-elias-castelnuovo-a-la-union-sovietica-en-1932/. Saítta, Sylvia. Hacia la revolución: viajeros argentinos de izquierda. Buenos Aires, Fondo de Cultura Económica, 2007. Rupprecht, Tobias. Soviet Internationalism after Stalin. Cambridge University Press, 2015. Rupprecht, Tobias. "Socialist high modernity and global stagnation: a shared history of Brazil and the Soviet Union during the Cold War". Journal of Global History, Volume 6, Issue 3, November 2011, pp. 505 - 528. DOI: https://doi.org/10.1017/S174002281100043X Tarcus, Horacio. Primeros viajeros al país de los soviets. Crónicas porteñas. 1920-1934. Buenos Aires: Ministerio de Cultura del Gobierno de la Ciudad Autónoma de Buenos Aires, 2017 Zourek, Michel. Praga y los intelectuales latinoamericanos (1947-1959). Rosario, Prohistoria, 2019, Esta base de datos recoge libros y artículos relacionados genéricamente con las escrituras del yo y cuya autoría recae en autores latinoamericanos, preferentemente quienes son reconocidos como escritores profesionales, aunque también se han incluido otros perfiles laborales. En ellos se detallan experiencias vitales acaecidas en los países socialistas europeos. En cuanto a su estado actual, consideramos que la base de datos se encuentra en proceso. Por ello, agradecemos cualquier observación que nos puedan hacer llegar para complementarla o mejorarla. Pueden escribir en este sentido a emilio.gallardo@csic.es, Este resultado es parte del proyecto de I+D+i Escritores latinoamericanos en los países socialistas europeos durante la Guerra Fría (PID2020-113994GB-I00), financiado por MCIN/ AEI/10.13039/501100011033/, No

Simulated data: For all the studies protein families (DNAK, DDL, TPIS, TRPA, TRXB, SH2). - The folder “*SimulatedANDinferredDATA” includes all simulated (true) protein sequence alignments and inferred with ProtASR under MF and the empirical JTT model (both for joint and marginal ASR) - The folder “*ComputedEnergiesFromData” includes the calculated energies of sequences of every MSA (files *.dat)., Real data: Files of the analysis based on real data are shown in the folder “RealData”, for the studied protein families (DNAK, DDL, TPIS, TRPA, TRXB). MSA and phylogenetic tree are in .fas/.nex formats and Newick format, respectively. Energies are shown in the .dat file and printed on the tree in files *Energies.tre (we recommend open them with FrigTree)., The computational reconstruction of ancestral proteins provides information on past biological events and has practical implications for biomedicine and biotechnology. Currently available tools for ancestral sequence reconstruction (ASR) are often based on empirical amino acid substitution models that assume that all sites evolve at the same rate and under the same process. However, this assumption is frequently violated because protein evolution is highly heterogeneous due to different selective constraints among sites. Here, we present ProtASR, a new evolutionary framework to infer ancestral protein sequences accounting for selection on protein stability. First, ProtASR generates site-specific substitution matrices through the structurally constrained mean-field substitution model (MF), which considers both unfolding and misfolding stability. We previously showed that MF models outperform empirical amino acid substitution models, as well as other structurally constrained substitution models, both in terms of likelihood and correctly inferring amino acid distributions across sites. In the second step, ProtASR adapts a well-established maximum-likelihood (ML) ASR procedure to infer ancestral proteins under MF models. A known bias of ML ASR methods is that they tend to overestimate the stability of ancestral proteins by under-estimating the frequency of deleterious mutations. We compared ProtASR under MF to two empirical substitution models (JTT and CAT), reconstructing the ancestral sequences of simulated proteins. ProtASR yields reconstructed proteins with less biased stabilities, which are significantly closer to those of the simulated proteins. Analysis of extant protein families suggests that folding stability evolves through time across protein families, potentially reflecting neutral fluctuation. Some families exhibit a more constant protein folding stability, while others are more variable. ProtASR is freely available from https://github.com/miguelarenas/protasr and includes detailed documentation and ready-to-use examples. It runs in seconds/minutes depending on protein length and alignment size., Peer reviewed

Table S1: Chemical compositions of the control wines without oenological additives (average values); Table S2: Chemical compositions of the oenological additives used in this study., Peer reviewed

Table S1. Columns used for the optimization of the HILIC × RP method used in the present research. Table S2. Identification proposed for the anthocyanins evaluated in the grape samples during ripening for a 10-week period. Figure S1. C18-RP 1DLC analysis of grape W9 (A) and wine (B). Blue: separation acquired at 280 nm; red: separation acquired at 520 nm. Figure S2. Chromatograms (280 nm) obtained for the HILIC separations obtained from a grape sample after optimization in a Zic-HILIC (A), diol (B) and silica (C) columns. Separation conditions are shown in inserts. Figure S3. Chromatograms (280 nm) obtained for the RP-based separations obtained from a grape sample after optimization in a PFP (A) and C18 (B) columns. Separation conditions are: flow rate 2 ml min-1 (Panel A) or 3 ml min-1 (panel B); elution with water (0.1% formic acid, solvent A) and acetonitrile (solvent B) as mobile phases. Specific gradients were shown in the respective chromatogram. Figure S4. 2D contour plots from the polyphenolic fraction of a grape sample (W10) obtained using different gradients and mobile phases composition in the second dimension, as indicated. Figure S5. 2D plots obtained under the optimum separation conditions for the grape samples of different ripening degree (W1 to W10)., Peer reviewed

[Introduction] Sunflower breeding for resistance to the parasitic plant sunflower broomrape (Orobanche cumana Wallr.) requires the identification of novel resistance genes. In this research, we conducted a genome-wide association study (GWAS) to identify QTLs associated with broomrape resistance., [Methods] The marker-trait associations were examined across a germplasm set composed of 104 sunflower accessions. They were genotyped with a 600k AXIOM® genome-wide array and evaluated for resistance to three populations of the parasite with varying levels of virulence (races EFR, FGV, and GTK) in two environments., [Results and Discussion] The analysis of the genetic structure of the germplasm set revealed the presence of two main groups. The application of optimized treatments based on the general linear model (GLM) and the mixed linear model (MLM) allowed the detection of 14 SNP markers significantly associated with broomrape resistance. The highest number of marker-trait associations were identified on chromosome 3, clustered in two different genomic regions of this chromosome. Other associations were identified on chromosomes 5, 10, 13, and 16. Candidate genes for the main genomic regions associated with broomrape resistance were studied and discussed. Particularly, two significant SNPs on chromosome 3 associated with races EFR and FGV were found at two tightly linked SWEET sugar transporter genes. The results of this study have confirmed the role of some QTL on resistance to sunflower broomrape and have revealed new ones that may play an important role in the development of durable resistance to this parasitic weed in sunflower., Peer reviewed