BUSCADOR RECOLECTA

Cylindrospermopsin (CYN) is a cyanotoxin with an increasing occurrence, and therefore it is important to elucidate its toxicity profile. CYN has been classified as a cytotoxin, although the scientific literature has already revealed that it affects a wide range of organs and systems. However, research on its potential immunotoxicity is still limited. Thus, this study aimed to evaluate the impact of CYN on two human cell lines representative of the immune system: THP-1 (monocytes) and Jurkat (lymphocytes). CYN reduced cell viability, leading to mean effective concentrations (EC50 24 h) of 6.00± 1.04 µM and 5.20± 1.20 µM for THP-1 and Jurkat cells, respectively, and induced cell death mainly by apoptosis in both experimental models. Moreover, CYN decreased the differentiation of monocytes to macrophages after 48 h of exposure. In addition, an up-regulation of the mRNA expression of different cytokines, such as interleukin (IL) 2, IL-8, tumor necrosis factor-alpha (TNF-α) and interferon-gamma (INF-ɣ), was also observed mainly after 24 h exposure in both cell lines. However, only an increase in TNF-_ in THP-1 supernatants was observed by ELISA. Overall, these results suggest the immunomodulatory activity of CYN in vitro. Therefore, further research is required to evaluate the impact of CYN on the human immune system. Dataset contains the numerical values of the results obtained regarding cytotoxicity, differentiation, cell death mechanisms (apoptosis/necrosis), gene expression and interleukin leakage., Numerical data of the results of cytotoxicity, differentiation, cell death mechanisms (apoptosis/necrosis), gene expression and interleukin leakage

Experimental data of copper leaching kinetics from non-pulverized printed circuit boards are compiled. They consist of the leached copper values as a function of time for the different tests performed. The copper concentrations were measured by atomic absorption spectrophotometry. Moreover, scanning electron microscope photomicrographs and energy dispersive X-ray spectroscopy data of a PCB sample has been included.

En este documento se presenta la herramienta metodológica empleada en el contexto del proyecto I+D "Atención a la diversidad y educación inclusiva en la Universidad. Diagnóstico y evaluación de indicadores de institucionalización” (referencia EDU2017-82862-R, 2018-2022) para realizar un análisis documental de las políticas universitarias en su abordaje de la inclusión educativa y la diversidad. Se describen, en primer lugar, los procedimientos de selección, en segundo lugar, los criterios de organización documental y, en tercer lugar, la estructura de categorías empleadas.

Here, experimental data for the different stages of a bioprocess for obtaining copper from printed circuit boards are compiled. Chemical analysis data to determine the chemical composition of samples and residues. Conditions and results of batch and continuous leaching, solvent extraction, biooxidation and electrowinning tests are included., One Microsoft Excel file with 6 sheets called: Composition, Batch, CSTR, Bio-oxidation, SX and EW.

El dasaset incluye los datos brutos de los experimentos western blot y ELISA realizados en el trabajo titulado "Anti-VEGF Effect of Bioactive Indolic Compounds and Hydroxytyrosol Metabolites". También se incluyen las medias y las desviaciones estándar., Datos numéricos

A three-step (rotor-stator-microfluidization-rotor stator) protocol was used to prepare 15% lemon essential oil in water emulgels using a mixture of Tween 80 and Span 20 surfactants as low molecular mass emulsifiers and 0.4% low-methoxyl citrus peel pectin as a gelling agent. Ca2+ was used as a gel-promoting agent. Different CaCl2/pectin mass ratio values from 0.3 to 0.7 were used. Emulgels showed a microstructure consisting of oil droplets embedded in a sheared gel matrix, as demonstrated by bright field optical microscopy. Laser diffraction tests showed multimodal particle size distributions due to the coexistence of oil droplets and gel-like particles. Multiple light scattering tests revealed that the physical stability of emulgels was longer as the CaCl2/pectin mass ratio decreased and that different destabilization mechanisms took place. Thus, incipient syneresis became more important with increasing CaCl2 concentration, but a parallel creaming mechanism was detected for CaCl2/pectin mass ratio values above 0.5. Dynamic viscoelastic and steady shear flow properties of the emulgels with the lowest and highest CaCl2/pectin mass ratio values were compared as a function of aging time. The lowest ratio yielded an emulgel with enhanced connectivity among fluid units as indicated by its wider linear viscoelastic region, higher storage modulus, loss modulus and viscosity values, and more shear thinning properties than those of the emulgel formulated with the highest CaCl2/pectin mass ratio. The evolution of the dynamic viscoelastic properties with aging time was consistent with the information provided by monitoring scans of backscattering as a function of sample height

The laboratory experiment “Valorization of waste with high Zn content” was designed to introduce the concept of circular economy and waste recovery to students of the Materials Engineering Degree at the University of Seville (Spain). This laboratory experiment has been carried out every academic year since 2015-16. This document contains the experimental data collected during the laboratory practices, which are necessary to respond to the questionnaire posed to the students. Likewise, the anonymized responses of the students to the pre- and post-laboratory questionnaires that were designed to evaluate the achievement of the pedagogical objectives are included.

The aim of this study was to assess the potential mutagenicity and genotoxicity of graphene oxide (GO) and reduced-graphene oxide (rGO) following the recommendations of the European Food Safety Authority (EFSA). Thus, the mouse lymphoma assay (MLA) and the micronucleus test (MN) were performed in L5178YTk ± cells, and the Caco-2 cells were used for the standard and modified comet assays. The results indicated that GO (0–250 μg/mL) was not mutagenic in the MLA. However, rGO revealed mutagenic activity from 250 μg/mL and 125 μg/mL after 4h and 24h of exposure, respectively. In the MN test, negative results were obtained for both compounds at the concentrations assayed (0–250 μg/mL) for GO/rGO. Moreover, no DNA strand breaks, or oxidative DNA damage were detected in Caco-2 cells exposed to GO (0–250 μg/mL) and rGO (0–176.3 μg/mL for 24h and 0–166.5 μg/mL for 48h). Dataset contains the numerical values of the results obtained regarding to MLA assay 4 and 24 h, MN test 24h and comet assay 24 and 48h., Numerical data of the results of MLA assay, MN test and comet assay

The cytotoxicity response of reduced graphene oxide (rGO) was investigated in monocytes (THP-1) and human T cells (Jurkat). A mean effective concentration (EC50-24 h) of 121.45 ± 11.39 μg/mL and 207.51 ± 21.67 μg/mL for cytotoxicity was obtained in THP-1 and Jurkat cells, respectively. rGO decreased THP-1 monocytes differentiation at the highest concentration after 48 h of exposure. Regarding the inflammatory response at genetic level, changes (up and downregulations) were observed depending on the cell line, the exposure time and the interleukins investigated. Apoptosis/ necrosis genes expression was not altered in THP-1 cells but in Jurkat cells BAX and BCL-2 were downregulated after 4h. Finally, rGO did not trigger a significant release of any cytokine at any exposure time assayed. Dataset contains the numerical values of the results obtained regarding cytotoxicity, differentiation, gene expression and interleukin leakage., Numerical data of the results of cytotoxicity, differentiation, gene expression and interleukin leakage