Resultados de investigación

To investigate differences in gene expression during flatfish metamorphosis we selected three key stages in turbot development: pre-metamorphic stage (15 dpf); climax of metamorphosis (30dpf); and post-metamorphic stage (57dpf). These fish were reared under standard production conditions at 18ºC. The migrant and non-migrant eyes and their respective surrounding tissue were directed for each stage and RNA were extracted. Libraries were sequenced on a BGISEQ-500 platform and single-end reads of 50 base pairs length were generated per sample, Flatfish have evolved their visual system to meet the demands of their environment through a fascinating process of metamorphosis. This morphological transformation involves a significant change in lifestyle from pelagic to benthic, accompanied by changes in body structure to achieve an asymmetrical flat shape. However, the fundamental change lies in the migration of one eye from its original position to the opposite location on the body. Although the role of thyroid hormone in triggering flatfish metamorphosis is well established, the specific gene regulatory network responsible for this extraordinary eye migration is still unknown. Here we show a global view through the creation of a general map of gene expression during the metamorphic process, encompassing both migrant and non-migrant eyes. Our research uncovers significant differences between migrant and non-migrant eyes of turbot in the pre-metamorphic and climax phases, emphasizing genetic disparities crucial for benthic adaptation, MCIN/AEI/10.13039/501100011033 (AGL2017-89648P and PID2021-126511OB-100 to J.R.) and “ERDF A way of making Europe”, by the “European Union”, No

Raw sequence reads data, Illumina sequencing of mussel hemocytes transcriptome after viral VHSV stimulation: three control and three infected individuals 24 hours after the stimulation, Peer reviewed

Peer reviewed

Protein sequences from transcription factors curated at https://footprintdb.eead.csic.es, footprintDB is developed by Alvaro Sebastian Yague and Bruno Contreras Moreira and it is maintained at the Computational & Structural Biology group (Zaragoza, Spain), with funding from ARAID, CSIC and the 2008 Euro Research Program [EUI2008-03612]., Peer reviewed

Este anexo incluye información relevante no incluida en el libro impreso., This compilation encompasses all records of inland water animals and plants in Madrid County over the years. It is an appendix to the book "Ecología acuática de Madrid", published by Editorial CSIC in 2020., Peer reviewed

Contents: Repo Contents. -- System Requirements. -- Installation Guide. -- Demo. -- License. -- Issues. -- Citation, Peer reviewed

Expression profiling by high throughput sequencing. -- To delve deeper into the biological mechanisms implicated in the MIA effects on neurodevelopment processes, we have generated neural stem cell lines from fetuses carried by pregnant mice treated with Poly I:C with the aim of investigating the alterations that persist in cultured neurons derived from the differentiation of these neural progenitors., To undertake a comprehensive examination of the molecular mechanisms governing the alterations induced by maternal immune activation (MIA) during gestation that impacts the subsequent neurodevelopment of progeny, we have devised an in vitro model based on neural stem cells (NSCs) sourced from fetuses carried by animals subjected to Poly I:C treatment. These neural progenitors demonstrate proliferative properties and can be effectively differentiated into both neurons and glial cells. Through transcriptomic, proteomic, and phosphoproteomic analyses conducted on these cellular models, in conjunction with counterparts from control treatments, discernible shifts in the expression levels of a specific subset of proteins implicated in neuronal function were elucidated. Noteworthy is the absence of congruence between these alterations and the transcriptomic findings, suggesting a potential dysregulation at the level of protein translation. Furthermore, a discernible discrepancy in the basal phosphorylation of proteins was observed between differentiated cells from both experimental groups, particularly within proteins associated with cytoskeletal architecture and synaptic functionality, notably those belonging to the MAP family. These observed alterations were found to correlate with changes in neuronal function, particularly with respect to synaptic plasticity and the establishment of neuronal synapses, thereby reaffirming previous assertions regarding the potential impact of MAP2 function modulation via its phosphorylation state on neuronal structure and function., Peer reviewed

Expression profiling by high throughput sequencing, We dissected hippocampus from WT and Daam1-MIC_KO adult mice and perform RNA-seq, To investigate the impact of Daam1 microexon removal on hippocampal function, we have generated RNA-seq data from adult male mice. Actin cytoskeleton dynamics is crucial for neurogenesis and neuronal function. Precise quantitative and qualitative regulation of actin polymerization is achieved by multiple actin-binding proteins, among which formins are particularly versatile. Here, we investigate how neuronal-specific splicing expands formin's functional diversity in the brain. We uncovered a highly conserved microexon in DAAM1 that extends the linker region of its FH2 domain, leading to remarkable changes in actin polymerization rates and structure. Microexon deletion causes neuritogenesis defects and increased calcium influx in in vitro differentiated mouse neurons, and mice carrying this deletion exhibit deficient long-term memory formation. These memory defects were associated with higher activity of DAAM1's interactor RhoA, increased ARC protein levels, postsynaptic deficiencies, fewer dendritic spines and impaired long-term potentiation. In summary, precise post-transcriptional regulation of DAAM1's FH2 domain is a novel mechanism for modulating actin polymerization in neural spinesneurons and is essential for proper brain function., Peer reviewed

Sampled and measured for AMS using the KLY-3S Kappabridge (AGICO, Czech Republic) and processed with the Anisoft 5.1.01 software (Chadima et al., 2018)., This study was funded by Project UKRIA-4D (PID2019-104693GB-I00) of the Spanish Ministry of Science and Innovation,, Peer reviewed

Sampling, stepwise demagnetization and measurement of the natural remanent magnetization. - Methods for processing the data: Characteristic components directions (ChRM) were calculated by principal component analyses (PCA, Kirschvink, 1980) after they were identified through visual inspection of orthogonal demagnetization plots (Zijderveld, 1967) using the VPD software (Ramón et al., 2017)., Peer reviewed