BUSCADOR RECOLECTA

Here you can found the two SPSS database to replicate the analyses for the validation of the ABC Connectedness to Nature Scale, validated in a paper published at the Environmental Education Research (doi: 10.1080/13504622.2022.2111407)., Peer reviewed

All images show projections of confocal sections, except D, I, L, O, and Q, which show single confocal sections. (A, B) The overexpression of GFP-kkv in the trachea leads to the presence of intracellular chitin vesicles at early stages (pink arrowheads) (A, A’). At later stages, intracellular chitin vesicles are not present, and chitin is deposited extracellularly in the lumen (blue arrowheads and inset) (B, B’). (C, C’) In exp reb mutants, the overexpression of GFP-kkv in the trachea produces intracellular chitin punctae until late stages (pink arrowheads). (D) The overexpression of GFP-kkv in salivary glands produces intracellular chitin vesicles (pink arrowheads). (E) Schematic representation of Exp protein. (F, G) In exp reb mutants, the expression of a wild-type form of exp/reb rescues the lack of extracellular chitin deposition (F, white arrow and inset), while expΔMH2/rebΔMH2 do not (G, white arrow points to absence of CBP). (H, I) The co-overexpression of GFP-kkv and expΔMH2 in control embryo does not produce morphogenetic defects in trachea (H) or extracellular chitin deposition in salivary glands (white arrow) (I); however, intracellular chitin punctae are present (pink arrowheads in H, I, and inset in H). (J) The coexpression of GFP-kkv and expΔMH2 in exp reb mutants produces intracellular chitin particles (pink arrowheads) but does not rescue the lack of extracellular chitin deposition. (K, L) RebΔMH2 localises apically in trachea (K) and in salivary glands (L). (M) MH2-reb is not able to rescue the absence of extracellular chitin deposition in exp reb mutants. (N, O) The simultaneous expression of MH2-reb and GFP-kkv does not produce morphogenetic defects or ectopic chitin deposition in trachea (N) and in salivary glands (white arrow in O), but intracellular chitin vesicles are present (pink arrowhead in O). (P, Q) MH2-Exp protein does not localize apically in trachea (P) or in salivary gland (Q). Scale bars A-C, F-H, J, M, N: 25 μm; D, I, K, L, O-Q: 10 μm., Peer reviewed

The excerpted portion of the immunoblot shown in the figures is highlighted by a black box., Peer reviewed

All images correspond to projections of confocal sections. (A-D) At early stages, overexpressed GFP-Kkv accumulates apically (white arrow in B) and in intracellular punctae (blue arrow in B), as endogenous Kkv (white and blue arrows in A), but also in the whole cell. At later stages, GFP-Kkv shows a pattern in stripes that corresponds to the taenidial folds (inset in D), in a comparable pattern to the endogenous Kkv (inset in C). Endogenous Kkv at late stages localises mainly apical and almost no intracellular punctae are detected (C). GFP-Kkv also localises mainly apical, but in addition, Kkv intracellular punctae are also detected (blue arrow in D). (E-H) In trachea, the simultaneous overexpression of reb and GFP-kkv anticipates chitin deposition (compare E and F). At later stages, this results in different morphogenetic defects like short and straight tubes and defects in branch fusion (compare H and G). (I) In salivary glands, the coexpression of reb and GFP-kkv promotes accumulation of chitin in the lumen. Scale bars: 10 μm., Peer reviewed

Calorimetric measurements, 1H field cycling relaxometry, and 2D 2H NMR spectra., Peer reviewed

The following supporting information: Figure S1: HPLC chromatograms of betalains and phenolic compounds from Opuntia stricta var. dillenii green extracts. The numbers associated to the peaks are according to Table S1; Table S1: Characterization of the main betalains and phenolic compounds present in Opuntia stricta var. dillenii green extracts analyzed by HPLC-DAD and HPLC-MS1 [5]; Table S2: Composition of the digestive phases and the enzymes used for the in vitro gastro-intestinal digestion assay by INFOGEST method; Table S3. Recovery of main betalains and phenolic compounds from Opuntia stricta var. dillenii green extracts (control) encapsulated by Formulation A and Formulation B after in vitro gastro-intestinal digestion., Peer reviewed

Supplementary Figures: Supplementary Figure 1. Short and long-term synaptic plasticity are preserved in the hippocampus of PD rats. (A) Pair pulse stimulation of the perforant pathway. Suprathreshold and equal intensity pairs of pulses (upper panel) are delivered at varying inter-stimulus time intervals (ISI). The results (lower panel) are expressed as the PS amplitude ratio (PS2/PS1). PS ratios below 1 indicate response depression and vice versa. Insets show pair pulse inhibition (left) and facilitation (right). (B) LTP of the perforant pathway. The upper panel shows the stimulation protocol used to induce LTP. Lower panel: synaptic potentiation in PD (orange) and control (black) rats quantified as the percentage increase of the PS amplitude after vs. before LTP induction. (C) Same as B but for the EPSP slope. (D) Evolution of the PS amplitude in response to perforant path stimulation before and 1 h after LTP induction. LTP protocol is applied at minute 6 (lasting 16 min) and PS amplitude is not tested until 1h later (grey shadow). The PS is normalized to pre-LTP measurements, and averaged across animals (n=5 per group). Data represents mean ± SEM. Supplementary Figure 2. Effect size of FA alterations in AUD patients analyzed with CSF correction (see Methods). Supplementary Figure 3. Effect size of FA alterations in a group of AUD patients chosen to avoid the age differences between groups. Supplementary Figure 4. Linear correlations between FA in the fimbria/fornix and cognitive variables: (A) NST, (B) TMT-A, (C) TMT-B, (D) number of perseverative errors in the WCST, (E) reaction times in the WCST, and (F) the Stroop test. (G) Correlation between FA in the fimbria/fornix and age. Supplementary Table 1. List of regions employed for the effect size analysis. Supplementary Table 2. List of cognitive test scores and age from AUD patients., Peer reviewed

This is the auxiliary online material to the research article: Brands et al. (2023): A global climate model performance atlas for the Southern Hemisphere extratropics based on regional atmospheric circulation patterns For further informaion, please see the README.txt file contained herein or contact Swen Brands, swen.brands@gmail.com, Peer reviewed

We initiate the study of a bulk-boundary eigenvalue problem for the Bilaplacian with a particular third order boundary condition that arises from the study of dynamical boundary conditions for the Cahn-Hilliard equation. First we consider continuity properties under parameter variation (in which the parameter also affects the domain of definition of the operator). Then we look at the ball and the annulus geometries (together with the punctured ball), obtaining the eigenvalues as solutions of a precise equation involving special functions. An interesting outcome of our analysis in the annulus case is the presence of a bifurcation from the zero eigenvalue depending on the size of the annulus., This work was initiated while C. Falcó was participating in the program “Introducción a la Investigación Severo Ochoa” grant at the Instituto de Ciencias Matemáticas (ICMAT). M.d.M. González acknowledges financial support from the Spanish Government, grant numbers MTM2017-85757-P, PID2020- 113596GB-I00; additionally, Grant RED2018-102650-T funded by MCIN/AEI/10.13039/501100011033, and the “Severo Ochoa Programme for Centers of Excellence in R&D” (CEX2019-000904-S)., With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2019-000904-S)., No

Experimental chronogram: Figure S1: Chronogram of the glutathione manipulation. BSO = buthionine sulfoximine. From (Romero Haro and Alonso-Alvarez 2015). DNA extraction protocol. Supplementary statistical analyses. Supplementary Results: Table S1; Table S2; Table S3; Table S4; Table S5; Figure S2; Figure S3; Figure S4; Figure S5; Figure S6; Figure S7; Figure S8. Interaction between early glutathione levels and telomere length on longevity, Peer reviewed