Resultados totales (Incluyendo duplicados): 33785
Encontrada(s) 3379 página(s)
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331427
Dataset. 2022

SUPPLEMENTARY INFORMATION FOR ENGINEERING PAN-HIV-1 NEUTRALIZATION POTENCY THROUGH MULTISPECIFIC ANTIBODY AVIDITY

  • Rujas, Edurne
  • Cui, Hong
  • Burnie, Jonathan
  • Burn Aschner, Clare
  • Zhao, Tiantian
  • Insausti, Sara
  • Muthuraman, Krithika
  • Semesi, Anthony
  • Ophel, Jasper
  • Seaman, Michael S.
  • Guzzo, Christina
  • Treanor, Bebhinn
  • Julien, Jean-Philippe
13 pages. -- This PDF file includes: Figures S1 to S7 and Tables S1 to S2. -- Fig. S1. Characterization of scFab-apoferritin fusions. -- Fig. S2. Multabody affinity-purification scheme. Protein A and Protein L sequential affinity purification. -- Fig. S3. Generation of a Multabody that cross-targets the HIV-1 Env and the CD4 receptor. -- Fig. S4. Biophysical characterization of HIV-1 Multabodies. Comparison of the Tm and Tagg temperatures of T-01/T-02 MB, 12-mer ferritin fusions, parental IgGs and the N6/PGDM1400x10E8v4 trispecific antibody. -- Fig. S5. Binding characteristics of IgGs binding to four different antigens. -- Fig. S6. Multabody v2 features. -- Fig. S7. PsV neutralization and inhibition of primary PBMC infection by Multabodies. -- Table S1. IC50 of individual and IgG mixtures, PGDM1400/N6x10E8v4 trispecific and HIV-1 Multabodies against a 14-PsV panel and 25-PsV panel (additional 11 HIV-1 strains highly resistant to PGDM1400). -- Table S2. Potency of parental and IgG mixtures and T-01 Multabody versions against a 118-PsV panel., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331427
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331427
HANDLE: http://hdl.handle.net/10261/331427
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331427
PMID: http://hdl.handle.net/10261/331427
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331427
Ver en: http://hdl.handle.net/10261/331427
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331427

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331430
Dataset. 2022

TABLE_2_EVOLUTIONARY DYNAMICS OF THE REPEATOME EXPLAINS CONTRASTING DIFFERENCES IN GENOME SIZES AND HYBRID AND POLYPLOID ORIGINS OF GRASS LOLIINAE LINEAGES.DOCX

  • Moreno Aguilar, María Fernanda
  • Inda, Luis A.
  • Sánchez-Rodríguez, Aminael
  • Arnelas, Itziar
  • Catalán, Pilar
Loliinae samples used in the repetitive DNA analysis. Genome skimming paired-end (PE) reads per sample and PE reads selected by Repeat Explorer 2 per sample in each of the comparative analyses of the four Loliinae groups: Loliinae, BL (broad-leaved Loliinae), FL (fine-leaved Loliinae), Schedonorus., The repeatome is composed of diverse families of repetitive DNA that keep signatures on the historical events that shaped the evolution of their hosting species. The cold seasonal Loliinae subtribe includes worldwide distributed taxa, some of which are the most important forage and lawn species (fescues and ray-grasses). The Loliinae are prone to hybridization and polyploidization. It has been observed a striking two-fold difference in genome size between the broad-leaved (BL) and fine-leaved (FL) Loliinae diploids and a general trend of genome reduction of some high polyploids. We have used genome skimming data to uncover the composition, abundance, and potential phylogenetic signal of repetitive elements across 47 representatives of the main Loliinae lineages. Independent and comparative analyses of repetitive sequences and of 5S rDNA loci were performed for all taxa under study and for four evolutionary Loliinae groups [Loliinae, Broad-leaved (BL), Fine-leaved (FL), and Schedonorus lineages]. Our data showed that the proportion of the genome covered by the repeatome in the Loliinae species was relatively high (average ∼ 51.8%), ranging from high percentages in some diploids (68.7%) to low percentages in some high-polyploids (30.7%), and that changes in their genome sizes were likely caused by gains or losses in their repeat elements. Ty3-gypsy Retand and Ty1-copia Angela retrotransposons were the most frequent repeat families in the Loliinae although the relatively more conservative Angela repeats presented the highest correlation of repeat content with genome size variation and the highest phylogenetic signal of the whole repeatome. By contrast, Athila retrotransposons presented evidence of recent proliferations almost exclusively in the Lolium clade. The repeatome evolutionary networks showed an overall topological congruence with the nuclear 35S rDNA phylogeny and a geographic-based structure for some lineages. The evolution of the Loliinae repeatome suggests a plausible scenario of recurrent allopolyploidizations followed by diploidizations that generated the large genome sizes of BL diploids as well as large genomic rearrangements in highly hybridogenous lineages that caused massive repeatome and genome contractions in the Schedonorus and Aulaxyper polyploids. Our study has contributed to disentangling the impact of the repeatome dynamics on the genome diversification and evolution of the Loliinae grasses., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331430
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331430
HANDLE: http://hdl.handle.net/10261/331430
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331430
PMID: http://hdl.handle.net/10261/331430
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331430
Ver en: http://hdl.handle.net/10261/331430
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331430

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331431
Dataset. 2022

TABLE_3_EVOLUTIONARY DYNAMICS OF THE REPEATOME EXPLAINS CONTRASTING DIFFERENCES IN GENOME SIZES AND HYBRID AND POLYPLOID ORIGINS OF GRASS LOLIINAE LINEAGES.XLSX

  • Moreno Aguilar, María Fernanda
  • Inda, Luis A.
  • Sánchez-Rodríguez, Aminael
  • Arnelas, Itziar
  • Catalán, Pilar
Repeat Explorer 2 comparative analysis. Repeat content data for top clusters (repeat families) in each of the four evolutionary groups of Loliinae: (A) Loliinae; (B) broad-leaved (BL) Loliinae; (C) fine-leaved (FL) Loliinae; (D) Schedonorus., The repeatome is composed of diverse families of repetitive DNA that keep signatures on the historical events that shaped the evolution of their hosting species. The cold seasonal Loliinae subtribe includes worldwide distributed taxa, some of which are the most important forage and lawn species (fescues and ray-grasses). The Loliinae are prone to hybridization and polyploidization. It has been observed a striking two-fold difference in genome size between the broad-leaved (BL) and fine-leaved (FL) Loliinae diploids and a general trend of genome reduction of some high polyploids. We have used genome skimming data to uncover the composition, abundance, and potential phylogenetic signal of repetitive elements across 47 representatives of the main Loliinae lineages. Independent and comparative analyses of repetitive sequences and of 5S rDNA loci were performed for all taxa under study and for four evolutionary Loliinae groups [Loliinae, Broad-leaved (BL), Fine-leaved (FL), and Schedonorus lineages]. Our data showed that the proportion of the genome covered by the repeatome in the Loliinae species was relatively high (average ∼ 51.8%), ranging from high percentages in some diploids (68.7%) to low percentages in some high-polyploids (30.7%), and that changes in their genome sizes were likely caused by gains or losses in their repeat elements. Ty3-gypsy Retand and Ty1-copia Angela retrotransposons were the most frequent repeat families in the Loliinae although the relatively more conservative Angela repeats presented the highest correlation of repeat content with genome size variation and the highest phylogenetic signal of the whole repeatome. By contrast, Athila retrotransposons presented evidence of recent proliferations almost exclusively in the Lolium clade. The repeatome evolutionary networks showed an overall topological congruence with the nuclear 35S rDNA phylogeny and a geographic-based structure for some lineages. The evolution of the Loliinae repeatome suggests a plausible scenario of recurrent allopolyploidizations followed by diploidizations that generated the large genome sizes of BL diploids as well as large genomic rearrangements in highly hybridogenous lineages that caused massive repeatome and genome contractions in the Schedonorus and Aulaxyper polyploids. Our study has contributed to disentangling the impact of the repeatome dynamics on the genome diversification and evolution of the Loliinae grasses., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331431
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331431
HANDLE: http://hdl.handle.net/10261/331431
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331431
PMID: http://hdl.handle.net/10261/331431
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331431
Ver en: http://hdl.handle.net/10261/331431
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331431

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331433
Dataset. 2021

RECOMBINATION STUDY BY MLH1 IMMUNOSTAINING OF MOUSE SPERMATOCYTES AFTER DIETARY TREATMENTS

  • Casa-Esperon, Elena de la
  • Belmonte-Tebar, Angela
  • San Martin Perez, Estefania
  • Nam Cha, Syonghyun
  • Soler, Ana J.
  • Singh, Nadia D.
The dataset is an Excel file with five sheets that contain the following information: Sheet 1 ("1st experiment, 3 strains"): MLH1 foci count per spermatocyte per mice, strain and diet Sheet 2 ("2nd experiment, B6 males"): MLH1 foci count per spermatocyte per C57BL/6 mice treated with two diets (2nd experiment). Columns indicate the mouse ID and number of spermatocytes analyzed in parenthesis. Sheet 3 ("intercrossover distances"): Interfocus distances in control mice (maintenance diet) of 3 strains. Values are shown as percentage of synaptonemal complex length. Sheet 4 ("synaptonemal c. length, 1st"): Total autosomal length of synaptonemal complexes per strain, control groups (maintenance diets) Sheet 5 ("synaptonemal c. length, 2nd"): Total autosomal length of synaptonemal complexes per diet in C57BL/6 mice (2nd experiment), We performed two studies: in the initial one, adult males from the three strains were analyzed for the effect of two diets on recombination (undernourishment (reduction to 50% daily intake) and breeding diets (Teklad Global 18% Protein Rodent Diet)) provided during 24 days relative to a control group kept ad libitum with maintenance diet (Teklad Global 14% Protein Rodent Maintenance Diet). After the 24-day diet period, adult male mice were euthanized by cervical dislocation and weighed. After removing and weighing the testes, chromosome spreads for immunostaining as previously described (Anderson et al. 1999; de Boer et al. 2009; Milano et al. 2019). MLH1 immunostaining allows for identification of about 90% of mammalian crossover sites (Anderson et al. 1999; Cole et al. 2012). All slides were imaged on a Zeiss LSM 710 confocal microscope and analyzed using Zeiss Zen lite software. Only mid and mid-late pachytene stage spermatocytes were scored. For each spermatocyte, we counted the number of foci localizing to the SC of the 19 autosomes (Anderson et al. 1999); total SC length and interfocus distances were also measured in autosomes only., Meiotic recombination is a critical process for sexually reproducing organisms. This exchange of genetic information between homologous chromosomes during meiosis is important not only because it generates genetic diversity, but also because it is often required for proper chromosome segregation. Consequently, the frequency and distribution of crossovers are tightly controlled to ensure fertility and offspring viability. However, in many systems it has been shown that environmental factors can alter the frequency of crossover events. We have explored for the first time the effect of dietary changes on crossover frequency per nucleus. Our study was performed in spermatocytes of 3 mouse inbred strains by analyzing the number and position of crossovers along the synaptonemal complexes, as well as the length of such synaptonemal complexes, by immunostaining with antibodies against MLH1 (which allows the identification of the crossover sites) and SYCP3 (a component of the synaptonemal complex). Our results show that male recombination rate is sensitive to dietary changes, and this sensitivity depends on the genetic background in mice. This is first to report a nutrition effect on genome-wide levels of recombination., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331433
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331433
HANDLE: http://hdl.handle.net/10261/331433
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331433
PMID: http://hdl.handle.net/10261/331433
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331433
Ver en: http://hdl.handle.net/10261/331433
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331433

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331434
Dataset. 2022

TABLE_4_EVOLUTIONARY DYNAMICS OF THE REPEATOME EXPLAINS CONTRASTING DIFFERENCES IN GENOME SIZES AND HYBRID AND POLYPLOID ORIGINS OF GRASS LOLIINAE LINEAGES.XLSX

  • Moreno Aguilar, María Fernanda
  • Inda, Luis A.
  • Sánchez-Rodríguez, Aminael
  • Arnelas, Itziar
  • Catalán, Pilar
Repeat Explorer 2 comparative analysis. Repeat content data for phylogenetically analyzed clusters (repeat families) in each of the four evolutionary groups of Loliinae: (A) Loliinae; (B) broad-leaved (BL) Loliinae; (C) fine-leaved (FL) Loliinae; (D) Schedonorus., The repeatome is composed of diverse families of repetitive DNA that keep signatures on the historical events that shaped the evolution of their hosting species. The cold seasonal Loliinae subtribe includes worldwide distributed taxa, some of which are the most important forage and lawn species (fescues and ray-grasses). The Loliinae are prone to hybridization and polyploidization. It has been observed a striking two-fold difference in genome size between the broad-leaved (BL) and fine-leaved (FL) Loliinae diploids and a general trend of genome reduction of some high polyploids. We have used genome skimming data to uncover the composition, abundance, and potential phylogenetic signal of repetitive elements across 47 representatives of the main Loliinae lineages. Independent and comparative analyses of repetitive sequences and of 5S rDNA loci were performed for all taxa under study and for four evolutionary Loliinae groups [Loliinae, Broad-leaved (BL), Fine-leaved (FL), and Schedonorus lineages]. Our data showed that the proportion of the genome covered by the repeatome in the Loliinae species was relatively high (average ∼ 51.8%), ranging from high percentages in some diploids (68.7%) to low percentages in some high-polyploids (30.7%), and that changes in their genome sizes were likely caused by gains or losses in their repeat elements. Ty3-gypsy Retand and Ty1-copia Angela retrotransposons were the most frequent repeat families in the Loliinae although the relatively more conservative Angela repeats presented the highest correlation of repeat content with genome size variation and the highest phylogenetic signal of the whole repeatome. By contrast, Athila retrotransposons presented evidence of recent proliferations almost exclusively in the Lolium clade. The repeatome evolutionary networks showed an overall topological congruence with the nuclear 35S rDNA phylogeny and a geographic-based structure for some lineages. The evolution of the Loliinae repeatome suggests a plausible scenario of recurrent allopolyploidizations followed by diploidizations that generated the large genome sizes of BL diploids as well as large genomic rearrangements in highly hybridogenous lineages that caused massive repeatome and genome contractions in the Schedonorus and Aulaxyper polyploids. Our study has contributed to disentangling the impact of the repeatome dynamics on the genome diversification and evolution of the Loliinae grasses., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331434
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331434
HANDLE: http://hdl.handle.net/10261/331434
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331434
PMID: http://hdl.handle.net/10261/331434
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331434
Ver en: http://hdl.handle.net/10261/331434
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331434

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331436
Dataset. 2022

SUPPLEMENTARY MATERIAL FOR BY-CATCH OF SEA TURTLES IN PACIFIC ARTISANAL FISHERY: TWO POINTS OF VIEW: FROM OBSERVER AND FISHERS

  • Carpio, Antonio J.
  • Álvarez Gutiérrez, Yamel
  • Serrano Rodríguez, Rocío
  • Vergara, M. Belen
  • Quintero, Erklin
  • Tortosa, Francisco S.
  • Rivas, Marga L.
ANNEX 1 - Questionnaire on sea turtle bycatch. ANNEX 2 - Content validation., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331436
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331436
HANDLE: http://hdl.handle.net/10261/331436
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331436
PMID: http://hdl.handle.net/10261/331436
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331436
Ver en: http://hdl.handle.net/10261/331436
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331436

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331437
Dataset. 2022

TABLE_5_EVOLUTIONARY DYNAMICS OF THE REPEATOME EXPLAINS CONTRASTING DIFFERENCES IN GENOME SIZES AND HYBRID AND POLYPLOID ORIGINS OF GRASS LOLIINAE LINEAGES.DOCX

  • Moreno Aguilar, María Fernanda
  • Inda, Luis A.
  • Sánchez-Rodríguez, Aminael
  • Arnelas, Itziar
  • Catalán, Pilar
Phylogenetic signal based on Blomberg’s K values of repeat cluster contents obtained from the comparative RE2 analysis of Loliinae samples assessed in each of the four Loliinae groups: (A) Loliinae (38 samples, 38 clusters), (B) Broad-leaved (BL) Loliinae (15 samples, 96 clusters), (C) fine-leaved (FL) Loliinae (17 samples, 122 clusters), (D) Schedonorus (16 samples, 167 clusters), using the phylosig option of the phytools R package. Cluster abundance values (number of PE reads) are indicated in Supplementary Table 4. K values close to one indicate phylogenetic signal, values close to zero phylogenetic independence, and values >1 more phylogenetic signal than expected. p-Values based on 1000 randomizations. Significant values are highlighted in bold., The repeatome is composed of diverse families of repetitive DNA that keep signatures on the historical events that shaped the evolution of their hosting species. The cold seasonal Loliinae subtribe includes worldwide distributed taxa, some of which are the most important forage and lawn species (fescues and ray-grasses). The Loliinae are prone to hybridization and polyploidization. It has been observed a striking two-fold difference in genome size between the broad-leaved (BL) and fine-leaved (FL) Loliinae diploids and a general trend of genome reduction of some high polyploids. We have used genome skimming data to uncover the composition, abundance, and potential phylogenetic signal of repetitive elements across 47 representatives of the main Loliinae lineages. Independent and comparative analyses of repetitive sequences and of 5S rDNA loci were performed for all taxa under study and for four evolutionary Loliinae groups [Loliinae, Broad-leaved (BL), Fine-leaved (FL), and Schedonorus lineages]. Our data showed that the proportion of the genome covered by the repeatome in the Loliinae species was relatively high (average ∼ 51.8%), ranging from high percentages in some diploids (68.7%) to low percentages in some high-polyploids (30.7%), and that changes in their genome sizes were likely caused by gains or losses in their repeat elements. Ty3-gypsy Retand and Ty1-copia Angela retrotransposons were the most frequent repeat families in the Loliinae although the relatively more conservative Angela repeats presented the highest correlation of repeat content with genome size variation and the highest phylogenetic signal of the whole repeatome. By contrast, Athila retrotransposons presented evidence of recent proliferations almost exclusively in the Lolium clade. The repeatome evolutionary networks showed an overall topological congruence with the nuclear 35S rDNA phylogeny and a geographic-based structure for some lineages. The evolution of the Loliinae repeatome suggests a plausible scenario of recurrent allopolyploidizations followed by diploidizations that generated the large genome sizes of BL diploids as well as large genomic rearrangements in highly hybridogenous lineages that caused massive repeatome and genome contractions in the Schedonorus and Aulaxyper polyploids. Our study has contributed to disentangling the impact of the repeatome dynamics on the genome diversification and evolution of the Loliinae grasses., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331437
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331437
HANDLE: http://hdl.handle.net/10261/331437
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331437
PMID: http://hdl.handle.net/10261/331437
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331437
Ver en: http://hdl.handle.net/10261/331437
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331437

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331446
Dataset. 2022

SUPPLEMENTAL MATERIAL FINE-TUNING OF THE HSC70-BASED HUMAN PROTEIN DISAGGREGASE MACHINERY BY THE DISTINCTIVE C-TERMINAL EXTENSION OF APG2

  • Cabrera, Yovana
  • Bernardo-Seisdedos, Ganeko
  • Dublang, Leire
  • Albesa-Jové, David
  • Orozco, Natalia
  • Viguera, Ana Rosa
  • Millet, Óscar
  • Muga, Arturo
  • Moro, Fernando
8 pages. -- Table 1. SAXS data collection and processing. -- Figure S1: Binding of nucleotides does not promote significant conformational changes in Apg2 and Apg2C tertiary structure. -- Figure S2: Characterization of the interaction of Apg2ΔC with Hsc70 in the absence of nucleotides by SPR. -- Fig. S3: NMR chemical shift perturbation analysis. -- Fig- S4: Apg2IDR interacts with Hsc70 with 2-fold reduced affinity in the presence of nucleotides., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331446
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331446
HANDLE: http://hdl.handle.net/10261/331446
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331446
PMID: http://hdl.handle.net/10261/331446
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331446
Ver en: http://hdl.handle.net/10261/331446
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331446

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331452
Dataset. 2022

SUPPORTING INFORMATION FOR ADV. SCI., DOI 10.1002/ADVS.202105170 IDENTIFICATION OF A NEW CHOLESTEROL-BINDING SITE WITHIN THE IFN-Γ RECEPTOR THAT IS REQUIRED FOR SIGNAL TRANSDUCTION

  • Morana, Ornella
  • Nieto, Jon Ander
  • Björkholm, Patrik
  • Serna, Jorge Bernardino de la
  • Terrones, Oihana
  • Arboleya, Aroa
  • Ciceri, Dalila
  • Rojo, Iratxe
  • Blouin, Cédric M.
  • Lamaze, Christophe
  • Lorizate, Maier
  • Contreras, F. Xabier
20 pages. -- Figure S1. Bifunctional lipid probes and workflow used for in vivo photoaffinity binding studies. -- Figure S2. Photolabeling experiments of non-lipid nanodomain marker (transferrin receptor) and lipid nanodomain marker (Cav-1) with bifunctional lipids and competition experiments between bifunctional analogues and cold lipids in living cells. -- Figure S3. Role of previously described chol-binding motif in IFN-γR2-chol interaction in vivo. -- Figure S4. Physiochemical analysis of IFN-γR2TMD wild type and mutants. -- Figure S5. Validation of the novel chol-binding domain localized within the IFN-γR2TMD. -- Figure S6. Characterization of IFN-γR1 and IFN-γR2 constructs for ID-PRIME and proof of principle. -- Figure S7. Chol binding is required for IFN-γR transmembrane signal activation and PDL1 cell surface exposure in response to IFN-γ stimulation. -- Figure S8. Subcellular localization of candidate chol-binding proteins. -- Supplementary Table 1. Single-and Multi-span motifs found using MOPRO. -- Supplementary Table 2. List of chol-binding protein candidates., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331452
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331452
HANDLE: http://hdl.handle.net/10261/331452
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331452
PMID: http://hdl.handle.net/10261/331452
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331452
Ver en: http://hdl.handle.net/10261/331452
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331452

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331454
Dataset. 2022

MEASUREMENT OF THE HIGGS BOSON WIDTH AND EVIDENCE OF ITS OFF-SHELL CONTRIBUTIONS TO ZZ PRODUCTION [DATASET]

  • Tumasyan, Armen
  • Adam, Wolfgang
  • Andrejkovic, Janik Walter
  • Bergauer, Thomas
  • Chatterjee, Suman
  • Damanakis, Konstantinos
  • Dragicevic, Marko
  • Escalante Del Valle, Alberto
  • Fruehwirth, Rudolf
  • Jeitler, Manfred
  • CMS Collaboration
Since the discovery of the Higgs boson in 2012, detailed studies of its properties have been ongoing. Besides its mass, its width - related to its lifetime - is an important parameter. One way to determine this quantity is by measuring its off-shell production, where the Higgs boson mass is far away from its nominal value, and relating it to its on-shell production, where the mass is close to the nominal value. Here, we report evidence for such off-shell contributions to the production cross section of two Z bosons with data from the CMS experiment at the CERN Large Hadron Collider. We constrain the total rate of the off-shell Higgs boson contribution beyond the Z boson pair production threshold, relative to its standard model expectation, to the interval [0.0061, 2.0] at 95% confidence level. The scenario with no off-shell contribution is excluded at a p-value of 0.0003 (3.6 standard deviations). We measure the width of the Higgs boson as Th = 3.2 (+2.4 / -1.7) MeV, in agreement with the standard model expectation of 4.1 MeV. In addition, we set constraints on anomalous Higgs boson couplings to W and Z boson pairs., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331454
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331454
HANDLE: http://hdl.handle.net/10261/331454
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331454
PMID: http://hdl.handle.net/10261/331454
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331454
Ver en: http://hdl.handle.net/10261/331454
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331454

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