BUSCADOR RECOLECTA

(A, A’) S9 triple>LanB1RNAi (B) egg chambers stained with anti-LanB1 antibody (green), the DNA marker Hoechst (blue) and the PC marker Fasciclin III (FasIII, red). (B–C’) Stills taken from live imaging of BCs from control (C306Gal4; slboGal4; tslGal4) and C306; slbo; tsl>LanB1RNAi egg chambers. (D) Quantification of the migration defects in egg chambers of the indicated genotypes. The statistical significance of differences was assessed with a t test. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A” and B”, 20 μm. The raw data underlying panel D are available in S1 Data., Peer reviewed

(A) Schematic drawing of an early S9 egg chamber illustrating the BCs (yellow), NCs (gray), FCs (purple), BM (green), and the position where AFM measurements were taken (arrow). (B) Comparison of the apparent elastic modulus K in egg chambers of the designated genotypes. (C) Schematic drawing of a middle S9 egg chamber illustrating the BCs (yellow), NCs (gray), FCs (purple), BM (green), the mirror region (pink square) and the positions where AFM measurements were taken, anterior to the mirr region (A, black arrow) and in the mirr region (C, pink arrow). (D, E) Comparison of the apparent elastic modulus K in the mirr region (C) and anterior to the mirr region (E), in 4 control mirrGal4 (D) and mirr>EHBP1mCh egg chambers. A minimum of 4 different readings (circles)/egg chamber were taken. Horizontal lines in B, D, and E represent mean values. The statistical significance of differences was assessed with a t test, * P value < 0.05, ** P value < 0.01, and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. The raw data underlying panels B, D, and E are available in S1 Data., Peer reviewed

(A) S10 control tjGal4 and (B) tj>LanB1RNAi (B) egg chambers stained with anti-LanB1 antibody (green), the DNA marker Hoechst (blue) and the F-actin marker Rhodamine-Phalloidin (F-actin, red). (C) Quantification of the LanB1 levels in egg chambers of the specified genotypes. The statistical significance of differences was assessed with a t test, *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A and B, 20 μm. The raw data underlying panel C are available in S1 Data., Peer reviewed

BC migration in tslGFP; tjGal4 and tslGFP; tj>AbiRNAi egg chambers. Scale bar, 20 μm., Peer reviewed

BCs were directly visualized using bright field, related to S8 Fig. Scale bar, 20 μm., Peer reviewed

BC migration in nos;hisYFP and nos>AbiRNAi;hisYFP egg chambers, related to S7 Fig. Scale bar, 20 μm., Peer reviewed

Movies correspond to the ablation experiment shown in Fig 4. NCs membranes are visualized with Resille-GFP. A cell bond between 2 control NCs is ablated. Upon laser ablation, GFP fluorescent is lost in the middle of the ablated bond. The movie continues 10 s after the cut and shows displacement of the vertexes. Images are taken every 0.5 s. Scale bar, 10 μm., Peer reviewed

BC cluster rotation in control (tslGFP; mirrGal4) and EHBP1mCh overexpressing (tslGFP; mirr>EHBP1mCh) egg chambers, related to Fig 3. Scale bar, 10 μm., Peer reviewed

Peer reviewed

BC migration in tslGFP; tjGal4 and tslGFP; tj>EHBP1mCh egg chambers, related to S3 Fig. tslGFP is in green and EHBP1mCh in red, related to S4 Fig. Scale bar, 20 μm., Peer reviewed