BUSCADOR RECOLECTA

The origin of the FtsZ/tubulin protein family was extremely relevant for life since these proteins are present in nearly all organisms, carrying out essential functions such as cell division or forming a major part of the cytoskeleton in eukaryotes. Therefore, investigating the early evolution of the FtsZ/tubulin protein family could reveal crucial aspects of the diversification of the three domains of life. In this study, we revisited the phylogenies of the FtsZ/tubulin protein family in an extensive prokaryotic diversity, focusing on the main evolutionary events that occurred during its evolution. We found evidence of its early origin in the last universal common ancestor since FtsZ was present in the last common ancestor of Bacteria and Archaea. In bacteria, ftsZ genes are genomically associated with the bacterial division gene cluster, while in archaea, ftsZ duplicated prior to archaeal diversification, and one of the copies is associated with protein biosynthesis genes. Archaea have expanded the FtsZ/tubulin protein family with sequences closely related to eukaryotic tubulins. In addition, we report novel CetZ-like groups in Halobacterota and Asgardarchaeota. Investigating the C-termini of prokaryotic paralogs basal to eukaryotic tubulins, we show that archaeal CetZ, as well as the plasmidic TubZ from Firmicutes, most likely originated from archaeal FtsZ. Finally, prokaryotic tubulins are restricted to Odinarchaeaota and Prosthecobacter species, and they seem to belong to different molecular systems. However, their phylogenies suggest that they are closely related to α/β-tubulins pointing to a potential ancestrality of these eukaryotic paralogs of tubulins, Peer reviewed

18 pages, Supporting information for the article https://doi.org/10.1016/j.scitotenv.2021.145020, Peer reviewed

able S1: Species, sample, coverage, non-conversion rate (%), and average DNA methylation levels (mCG/CG) for all datasets generated in this study. Table S2: Genomic location of all differentially methylated regions (DMRs) identified in the medaka genome. Table S3: DMR-linked genes in zebrafish and medaka genomes., Peer reviewed

40 pages, Supplementary information for the article https://doi.org/10.1038/s41598-021-02328-6, Approach.-- Standardization of indicators,-- Calculation of index values.-- Socioeconomic indicators and factors.-- Institutional Indicators and Factors.-- Resilience factors summary.-- Index performance.-- Additional analyses.-- Bibliography, Peer reviewed

Additional file 1: Table S1. Table of number of genes, and genes in KEGG signaling pathways contained in HiPathia R package sharing an increasing number of RP-HPO terms., Consejería de Salud y Consumo, Junta de Andalucía, H2020 Marie Skłodowska-Curie Actions, Ministerio de Ciencia e Innovación, Instituto de Salud Carlos III, Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana, Peer reviewed

[Description of methods used for collection/generation of data] For the heat budget analysis, we utilized temperature tendency terms available in the Modular Ocean Model version 4p1 (MOM4p1). Heat flux anomalies (in W m-2) for each term were averaged over a time step at a given ocean grid cell, following Griffies et al. (2015). Subsequently, we calculated anomalies for each heat term relative to their seasonal cycles. These anoalies were then averaged separately over the days corresponding to the onset phase (i.e., heat build-up) and the decay phase (i.e., heat dissipation) of the marine heatwaves (MHWs). To study causal interactions between the physical variables (Max. SSTA, SIC, and MLD) and net primary production (NPP) rates, we applied Dynamic Empirical Modelling (EDM). Specifically, we utilised the Convergent Cross Mapping (CCM) method, as defined by Sugihara et al. (2012). Following a sensitivity test, we adjusted the time delays (τ) and embedding dimension (E) to 3 and 6, respectively., File List: MOM4p1; CCM., Peer reviewed

(DREM³L) Drug REpurposing using Mechanistic Models of signal transduction and Machine Learning, The authors acknowledge Junta de Andalucía for the postdoctoral contract of Carlos Loucera (PAIDI2020- DOC_00350) co-funded by the European Social Fund (FSE) 2014-2020., Peer reviewed

Figure S1. Identification of flagellaless GR4flaAB-derivative transposants impaired in the response to volatile 2-tridecanone (2-TDC). Figure S2. Multiple sequence alignment by MUSCLE of DnaJ amino acid sequences from different bacterial species Figure S3. Growth of S. meliloti dnaJ mutants and their parental strains on solid and liquid media. Figure S4. Effect of H2O2 on S. meliloti GR4 and GR4flaAB cell survival. Figure S5. Appearance of alfalfa plants inoculated with S. meliloti dnaJ mutant strains at the end of the nodulation kinetics experiment. Figure S6. Complementation of the symbiotic phenotype of S. meliloti dnaJ mutants. Table S1. Bacterial strains and plasmids used in this study. Table S2. List of primers used in this study, Peer reviewed

Supplementary Figure 1. GFP+ cells engraftment and mFVIII production correlation in transplanted mice. Supplementary Figure 2. Bleeding assay in newborn HA following transplantation. Supplementary Figure 3. Bleeding assay in adult HA following transplantation. Supplementary table 1. List of antibodies used for immunofluorescence and flow cytometry. Supplementary table 2. Engraftment (% GFP+ cells) and correction (% mFVIII activity) levels in plasma of newborn mice according to BU dosage and cell transplantation., Peer reviewed

Appendix of Emerging Microbes & Infections 13: 2295389 (2024), Rat hepatitis E virus (ratHEV; species Rocahepevirus ratti) is considered a newly emerging cause of acute hepatitis of zoonotic origin. ratHEV infection of people living with HIV (PLWH) might portend a worse, as with hepatitis E virus (HEV; species Paslahepevirus balayani), and consequently this group may constitute a high-risk population. We aimed to evaluate the prevalence of ratHEV by measuring viral RNA and specific IgG antibodies in a large Spanish cohort of PLWH. Multicentre study conducted in Spain evaluating PLWHIV included in the Spanish AIDS Research Network (CoRIS). Patients were evaluated for ratHEV infection using PCR at baseline and anti-ratHEV IgG by dot blot analysis to evaluate exposure to ratHEV strains. Patients with detectable ratHEV RNA were followed-up to evaluate persistence of viremia and IgG seroconversion. Eight-hundred and forty-two individuals were tested. A total of 9 individuals showed specific IgG antibodies against ratHEV, supposing a prevalence of 1.1 (95% CI; 0.5%−2.1%). Of these, only one was reactive to HEV IgG antibodies by ELISA. One sample was positive for ratHEV RNA (prevalence of infection: 0.1%; 95% CI: 0.08%−0.7%). The case was a man who had sex with men exhibiting a slightly increased alanine transaminase level (49 IU/L) as only biochemical alteration. In the follow-up, the patients showed undetectable ratHEV RNA and seroconversion to specific ratHEV IgG antibodies. Our study shows that ratHEV is geographical broadly distributed in Spain, representing a potential zoonotic threat., This work was supported by the Andalusian General Secretariat for Research, Development, and Innovation in Health (PI-0287-2019), the Spanish Ministry of Health (RD12/0017/0012), co-financed by European Regional Development Fund (ERDF), and the Carlos III Health Institute (Research Project grant numbers: PI21/00793 and PI22/01098). Projects “PI21/00793” and “PI22/01098” were funded by Carlos III Health Institute (ISCIII) and co-funded by the European Union. ARJ is the recipient of a “Miguel Servet” Research Contract by the Spanish Ministry of Sciences (CP18/00111). JCG is supported by the CIBERINFEC (CB21/13/00083), Carlos III Health Institute, Spanish Ministry os Science and NextGenerationEU. MCJ is the recipient of a PFIS predoctoral grant (FI22/00180) from the Carlos III Health Institute and co-funded by the European Union. DCM is the recipient of a “Rio-Hortega” (CM22/00176) grant from the Carlos III Health Institute and co-funded by the European Union. PLL is the recipient of a “Margarita Salas” contract funded by NextGeneration EU. TGG is recipient of a “Ramon y Cajal” contract funded by MCIN/AEI/10.13039/501100011033 and NextGeneration EU/PRTR. The laboratory of R.G.U. is supported by DZIF Thematic Translational Unit (TTU) “Emerging Infections” (grant number 01.808; awarded to R.G.U.). The HIV BioBank is supported by Carlos III Health Institute (PT20/00138) and Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN (CB22/01/00041). CoRIS cohort is supported by CIBER (CB21/13/00091), Carlos III Health Institute, Spanish Ministry of Sciences NextGenerationEU., Peer reviewed