BUSCADOR RECOLECTA

[Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations., [Methods]: We evaluated the performance of two SFC and MC panels, which contained 21 common markers, for the identification and subsetting of blood IMC populations. Based on unsupervised clustering analysis, we systematically identified 24 leukocyte populations, including 21 IMC subsets, regardless of the cytometry technique., [Results]: Overall, comparable results were observed between the two technologies regarding the relative distribution of these cell populations and the staining resolution of individual markers (Pearson’s ρ=0.99 and 0.55, respectively). However, minor differences were observed between the two techniques regarding intra-measurement variability (median coefficient of variation of 42.5% vs. 68.0% in SFC and MC, respectively; p<0.0001) and reproducibility, which were most likely due to the significantly longer acquisition times (median 16 min vs. 159 min) and lower recovery rates (median 53.1% vs. 26.8%) associated with SFC vs. MC., [Discussion]: Altogether, our results show a good correlation between SFC and MC for the identification, enumeration and characterization of IMC in blood, based on large panels (>20) of antibody reagents., Peer reviewed

(A) CBK1-T743E cdc15-2 (EW1447), (B) lre1Δ cdc15-2 (YMF3857), and (C) fir1Δ cdc15-2 (YMS3792) cells were grown in YPD and arrested in late anaphase by raising the temperature to 37 °C before the addition of rapamycin to half of the culture for 20 min. Subsequently, to allow progression through the cell cycle, cells were released in the absence (−) or presence (+) of rapamycin. Samples were taken at the specified times to determine cell-cycle progression by flow cytometry. FACS graphs can be found in the supplementary FACS file (S1 File)., journal.pbio.3002263.s003.pdf, Peer reviewed

Data is provided in the recent OBIS-ENV-DATA format, and comprises 8913 georeferenced positions associated with 3195 occurrences of 44 marine taxa.-- 3 files, The CETUS dataset contains effort-based occurrence records collected during a cetacean monitoring programme in the Eastern North Atlantic, since 2012, This research was partially funded by the EU FEDER/FEMP and Portuguese Foundation for Science and Technology (FCT) under the Portugal2020 (Lisboa2020, Algarve2020 and MAR2020) Programme through project OBSERVA.PT (MAR-01.04.02-FEAMP-0002), Peer reviewed

11 páginas, 1 tabla, 2 figuras, Peer reviewed

A. Experimental Part: materials, synthesis of gold bipyramids, partial etching of gold bipyramids, and instrumentation; B. Modeling: volume calculation in the geometric inversion model, three-descriptor analysis of bipyramid shapes, and model aspect ratios; C. Simulation of Optical Properties., Peer reviewed

Datasets Dietary Carbon Incorporation in Ichthyocarbonate.zip contains: Mendeley-Ichthyocarbonate.csv Mendeley-DaysPastFeeding-C.csv Mendeley-DaysPastFeeding-MgCO3.csv Mendeley-Diet.csv, Datasets accompanying manuscript accepted for publication in Science of the Total Environment. Manuscript is titled: Implications of dietary carbon incorporation in fish carbonates for the global carbon cycle. Datasets include: 1. Mendeley-Ichthyocarbonate.csv: Presents ichthyocarbonate compositional characteristics, including stable carbon isotope values of the carbonate and associated organic matter, %TOC, and %carbonate (NM stands for not measured). 2. Mendeley-DaysPastFeeding-C.csv: Presents stable carbon isotope values of ichthyocarbonate measured in successive days since last meal, 3. Mendeley-Dietcsv: Presents compositional characteristics of diet fed to experimental fish, and 4. Mendeley-DaysPastFeeding.csv: Presents d-spacing and mol%MgCO3 calculated from results of XRD analyses., Peer reviewed

MMC S1. The online supplementary materials show the optimized atomic coordinate and the converged lattice thermal conductivity., Peer reviewed

[Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations., [Methods]: We evaluated the performance of two SFC and MC panels, which contained 21 common markers, for the identification and subsetting of blood IMC populations. Based on unsupervised clustering analysis, we systematically identified 24 leukocyte populations, including 21 IMC subsets, regardless of the cytometry technique., [Results]: Overall, comparable results were observed between the two technologies regarding the relative distribution of these cell populations and the staining resolution of individual markers (Pearson’s ρ=0.99 and 0.55, respectively). However, minor differences were observed between the two techniques regarding intra-measurement variability (median coefficient of variation of 42.5% vs. 68.0% in SFC and MC, respectively; p<0.0001) and reproducibility, which were most likely due to the significantly longer acquisition times (median 16 min vs. 159 min) and lower recovery rates (median 53.1% vs. 26.8%) associated with SFC vs. MC., [Discussion]: Altogether, our results show a good correlation between SFC and MC for the identification, enumeration and characterization of IMC in blood, based on large panels (>20) of antibody reagents., Peer reviewed

[Introduction]: Monitoring of innate myeloid cells (IMC) is broadly applied in basic and translational research, as well as in diagnostic patient care. Due to their immunophenotypic heterogeneity and biological plasticity, analysis of IMC populations typically requires large panels of markers. Currently, two cytometry-based techniques allow for the simultaneous detection of ≥40 markers: spectral flow cytometry (SFC) and mass cytometry (MC). However, little is known about the comparability of SFC and MC in studying IMC populations., [Methods]: We evaluated the performance of two SFC and MC panels, which contained 21 common markers, for the identification and subsetting of blood IMC populations. Based on unsupervised clustering analysis, we systematically identified 24 leukocyte populations, including 21 IMC subsets, regardless of the cytometry technique., [Results]: Overall, comparable results were observed between the two technologies regarding the relative distribution of these cell populations and the staining resolution of individual markers (Pearson’s ρ=0.99 and 0.55, respectively). However, minor differences were observed between the two techniques regarding intra-measurement variability (median coefficient of variation of 42.5% vs. 68.0% in SFC and MC, respectively; p<0.0001) and reproducibility, which were most likely due to the significantly longer acquisition times (median 16 min vs. 159 min) and lower recovery rates (median 53.1% vs. 26.8%) associated with SFC vs. MC., [Discussion]: Altogether, our results show a good correlation between SFC and MC for the identification, enumeration and characterization of IMC in blood, based on large panels (>20) of antibody reagents., Peer reviewed

Computational Details, Supplemental Figures, Supplemental Tables., Peer reviewed