BUSCADOR RECOLECTA

Movies correspond to the ablation experiment shown in Fig 5. The membranes on the apical side of FCs are visualized with Resille-GFP. A cell bond between 2 control FCs is ablated. GFP fluorescent is lost in the middle of the ablated bond upon laser ablation. The movie continues 10 s after the cut and shows displacement of the vertexes. Movie length and frame rate are as described for Movie S11. Scale bar, 5 μm., Peer reviewed

Movies correspond to the ablation experiment shown in Fig 5. The membranes on the basal side of FCs are visualized with Resille-GFP. A cell bond between 2 control FCs is ablated. GFP fluorescent is lost in the middle of the ablated bond upon laser ablation. The movie continues 10 s after the cut and shows displacement of the vertexes. Images are taken every 0.5 s. Scale bar, 5 μm., Peer reviewed

[ES] El conjunto de datos está dividido en tres subcarpetas, dentro de las cuales los datos se encuentran agrupados por especies: Eggs: Proporciona más de 900 imágenes en formato RAW en el rango visual del espectro visible de puestas completas sobre fondo negro, correspondientes a 14 especies distintas. Nests: Proporciona más de 800 imágenes de nidos de 6 especies distintas, tomadas en su entorno desde 1 metro de distancia y a 70 cm de altura. En este segundo conjunto de imágenes, se incluyen imágenes de los nidos tanto en el rango visible como en ultravioleta cercano. Incubating Behavior: Proporciona más de 950,000 imágenes en formato jpg correspondientes a secuencias de vigilancia del comportamiento de incubación de 5 especies distintas, junto con información de las condiciones ambientales. [EN] The dataset is divided into three subfolders, within which the data is grouped by species: Eggs: Provides over 900 images in RAW format within the visible spectrum of complete clutches on a black background, corresponding to 14 different species. Nests: Provides over 800 images of nests from 6 different species, taken in their environment from 1 meter away and at a height of 70 cm. This set includes images of the nests in both the visible range and near-ultraviolet. Incubating Behavior: Provides over 950,000 jpg images corresponding to surveillance sequences of incubation behavior from 5 different species, along with information on environmental conditions., [ES] Este conjunto de datos, generado en el marco del proyecto CAMNID: CGL2017-83518-P, Plan Nacional I+D+i, contiene información visual sobre huevos, nidos y comportamiento durante la incubación de diferentes especies de aves de litoral (shorebirds). Permite la realización de análisis de coloración y moteado, entre otros, para las especies en estudio. Asimismo, se proporcionan secuencias de imágenes de monitorización de diversos nidos de estas especies a lo largo de 3 horas (entre las 12:00 y las 15:00) junto con las condiciones ambientales durante la vigilancia. Esto permite la realización de estudios de los comportamientos de estas aves durante la incubación, abriendo la posibilidad de estudiar diversos mecanismos de mitigación de estrés térmico o las alternancias parentales macho/hembra durante la incubación en función de las condiciones ambientales, [EN] This dataset, generated within the framework of the CAMNID project: CGL2017-83518-P, Plan Nacional I+D+i, contains visual information on eggs, nests, and incubation behavior of various shorebird species. It enables color and spotting pattern analyses, among other studies, for the species included. Additionally, it provides image sequences monitoring various nests of these species over a 3-hour period (from 12:00 to 15:00) along with the environmental conditions during the surveillance. This allows for studies of the birds' behavior during incubation, opening the possibility to study mechanisms of thermal stress mitigation and male/female parental alternation during incubation based on environmental conditions., CAMNID PROJECT: Grant: CGL2017-83518-P, Plan Nacional I+D+i: EFECTOS DE LAS INVASIONES GEOLOGICAS DE ORIGEN ANTROPICO SOBRE LAS ESTRATEGIAS DE CAMUFLAJE DE NIDOS EN EL CHORLITEJO PATINEGRO CHARADRIUS ALEXANDRINUS, The dataset is comprissed of three folders. Eggs, Nests, and BehavioralMonitoring. ** Folder: Eggs: Photographs of shorebird eggs from a zenital view in raw format. - INFORMATION_TABLE.xlsx: Excel file with information about each picture in the Eggs folder subset. Image name can be used as unique identifier. - Folders corresponding to eggs of 14 different species. Species name corresponding to folder name. - Inside each folder, a single RAW format image file for each egg is provided ** Folder: Nests: Zenital pictures of shorebirds nests in natural environments both in the Vis and UV wavelength ranges. - INFORMATION_TABLE.xlsx: Excel file with information about each picture in the Nests folder subset. Image name can be used as unique identifier. - Folders corresponding to 6 different species. Species name corresponding to folder name. - Inside each folder, two images per nest are provided. One for the visible wavelength range ({ImageName}_VIS.ARW) and another one for the UV range ({ImageName}_UV.ARW) **Folder: BehavioralMonitoring: Pictures showing the incubating activity of shorebird species. Additionally, environmental conditions are also provided. - README.txt: Readme file providing the description of each folder in the dataset - Folders corresponding to 5 shorebirds species being monitored. - Inside each species folder: ---subfolder: DATE_OF_OBSERVATION: | |---subfolder: DATALOGGERCODE (DTXX (XX from 0 to 9)) | |____FRAME_XXX_STD.jpg: XXXth Picture taken under normal conditions | |____YYY_ACT.jpg: YYYth Picture corresponding to a special activitity condition | |____DATA_LOGGER.txt: csv file containing enviromental conditions during this experiment. Updated every 100s | |____DTXX.gpx: GPS position of this observation (gpx standard format) | |____DTXX_GPS.txt: GPS position for this observation in txt format (the gpx file is not always available), Peer reviewed

BCs were directly visualized using bright field, related to S8 Fig. Scale bar, 20 μm., Peer reviewed

BC migration in nos;hisYFP and nos>AbiRNAi;hisYFP egg chambers, related to S7 Fig. Scale bar, 20 μm., Peer reviewed

Movies correspond to the ablation experiment shown in Fig 4. NCs membranes are visualized with Resille-GFP. A cell bond between 2 control NCs is ablated. Upon laser ablation, GFP fluorescent is lost in the middle of the ablated bond. The movie continues 10 s after the cut and shows displacement of the vertexes. Images are taken every 0.5 s. Scale bar, 10 μm., Peer reviewed

BC cluster rotation in control (tslGFP; mirrGal4) and EHBP1mCh overexpressing (tslGFP; mirr>EHBP1mCh) egg chambers, related to Fig 3. Scale bar, 10 μm., Peer reviewed

Peer reviewed

Current clinical guidelines support the concomitant administration of seasonal influenza vaccines and COVID-19 mRNA boosters vaccine. Whether dual vaccination may impact vaccine immunogenicity due to an interference between influenza or SARS-CoV-2 antigens is unknown. We aimed to understand the impact of mRNA COVID-19 vaccines administered concomitantly on the immune response to influenza vaccines. For this, 128 volunteers were vaccinated during the 22-23 influenza season. Three groups of vaccination were assembled: FLU vaccine only (46, 35%) versus volunteers that received the mRNA bivalent COVID-19 vaccines concomitantly to seasonal influenza vaccines, FluCOVID vaccine in the same arm (42, 33%) or different arm (40, 31%), respectively. Sera and whole blood were obtained the day of vaccination, +7, and +28 days after for antibody and T cells response quantification. As expected, side effects were increased in individuals who received the FluCOVID vaccine as compared to FLU vaccine only based on the known reactogenicity of mRNA vaccines. In general, antibody levels were high at 4 weeks post-vaccination and differences were found only for the H3N2 virus when administered in different arms compared to the other groups at day 28 post-vaccination. Additionally, our data showed that subjects that received the FluCOVID vaccine in different arm tended to have better antibody induction than those receiving FLU vaccines for H3N2 virus in the absence of pre-existing immunity. Furthermore, no notable differences in the influenza-specific cellular immune response were found for any of the vaccination groups. Our data supports the concomitant administration of seasonal influenza and mRNA COVID-19 vaccines., This work was supported by American Lung Association: [COVID-1034091]. Centro de Investigación Biomedica en Red de Enfermedades Infecciosas, CIBERINFEC, Instituto de Salud Carlos III, Ministerio de Ciencia e Innovacion and Union Europea – NextGeneration EU (Grant Number CB21/13/00006); Consejeria de Economia, Conocimiento, Empresas y Universidad, Secretaria General de Universidades, Investigacion y Tecnologia, Junta de Andalucia, Spain (P18-RT-3320). SEM-CIVIC National Institute of Allergy and Infectious Diseases: [Grant Number 75N93019C00051]. CEIRR-CRIPT: [75N93021C00014]., Peer reviewed

BC migration in tslGFP; mirrGal4 and tslGFP; mirr>EHBP1mCh egg chambers. tslGFP is in green and EHBP1mCh in red, related to Fig 2. Scale bar, 20 μm., Peer reviewed