Resultados totales (Incluyendo duplicados): 34260
Encontrada(s) 3426 página(s)
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311369
Dataset. 2022

TABLE_2_KNOCK-OUT OF CMNAC-NOR AFFECTS MELON CLIMACTERIC FRUIT RIPENING.XLSX

  • Liu, Bin
  • Santo Domingo, Miguel
  • Mayobre, Carlos
  • Martín-Hernández, Ana Montserrat
  • Pujol, Marta
  • García-Mas, Jordi
1 table. -- Supplementary Table S2: VOCs detected in melon flesh samples. A: List of VOCs detected. B: Relative content of VOCs (ng/g frozen tissue)., Fruit ripening is an important process that affects fruit quality. A QTL in melon, ETHQV6.3, involved in climacteric ripening regulation, has been found to be encoded by CmNAC-NOR, a homologue of the tomato NOR gene. To further investigate CmNAC-NOR function, we obtained two CRISPR/Cas9-mediated mutants (nor-3 and nor-1) in the climacteric Védrantais background. nor-3, containing a 3-bp deletion altering the NAC domain A, resulted in ~8 days delay in ripening without affecting fruit quality. In contrast, the 1-bp deletion in nor-1 resulted in a fully disrupted NAC domain, which completely blocked climacteric ripening. The nor-1 fruits did not produce ethylene, no abscission layer was formed and there was no external color change. Additionally, volatile components were dramatically altered, seeds were not well developed and flesh firmness was also altered. There was a delay in fruit ripening with the nor-1 allele in heterozygosis of ~20 days. Our results provide new information regarding the function of CmNAC-NOR in melon fruit ripening, suggesting that it is a potential target for modulating shelf life in commercial climacteric melon varieties., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311369
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311369
HANDLE: http://hdl.handle.net/10261/311369
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311369
PMID: http://hdl.handle.net/10261/311369
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311369
Ver en: http://hdl.handle.net/10261/311369
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311369

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311371
Dataset. 2023

RAW DATA ON THE EFFECT OF RESOURCE AVAILABILITY AND HERBIVORY ON THE DEFENCE-GROWTH-REPRODUCTION TRADE-OFF OF A MASTING MEDITERRANEAN PINE

  • Larrinaga, Asier R.
  • Sampedro Pérez, Luis
  • Zas Arregui, Rafael
[General methods] The full collection of six csv files contains all the raw data used in the paper “Resource availability and herbivory alter defence-growth-reproduction trade-offs in a masting Mediterranean pine”, which is now in its pre-print version. In this article we report the results of two parallel experiments we carried out to independently asses the effect of nutrient availability and different defence induction treatments on the three-way trade-off. Thirty six ramets were allocated to the fertilization experiment, with 12 ramets of each of three genotypes being randomly assigned to one of three blocks and one of two treatments (fertilization and control). Within each block, ramets were spatially grouped in two treatment plots. Each treatment by genotype combination comprises six ramets, two per block. The remaining 45 ramets were included in the defence induction experiment and randomly assigned to one of three blocks and one of five induction treatments. Within each block, ramets were spatially grouped in five treatment plots. Each treatment by genotype combination had three ramets, one per block. In the fertilization experiment we had only two treatments (fertilized vs control). Fertilization was accomplished by applying Fertimon Rojo (Soaga S.L. 2-10-16 NPK + 2.8% Mg) and a liquid 100-20-70 NPK fertilizer applied to the substrate at the base of the trunk of each tree (see Sampedro et al. 2011 for details on this fertilizer solution). The foliar fertilization continued until the end of the experiment. In january 2010, 125 additional grams of Osmocote (KB®, Scotts, NPK 17-09-11 + 2% MgO) were also added to each tree. Fertilization was maintained throughout the whole monitoring period (2010-2015). Five treatments were assigned to the herbivory simulation experiment (applied during the first two years, 2010-2011): • Needle clipping (repeated cutting of needles tips, around 1-2 cm). • Methyl jasmonate application (brushing a 25 mM solution on debarked areas of the trunk; see details in Vázquez-González et al. 2021). • Low intensity mechanical wounding of the trunk involved making three 5 mm circular holes in the bark and phloem. • High intensity mechanical wounding of the trunk, throug six 5 mm circular hole in the bark and phloem Ramets were monitored from 2010 to 2015. Below, we provide specific details on the methods associated to its data-set and the variables it includes., [growth.csv] Ramet growth was monitored for six years, beginning in 2010. Growth monitoring involved measuring maximum tree height and stem diameter at one meter height and two orthogonal directions. Tree height was measured to the nearest dm using a telescopic measuring rod, while tree callipers were used to measure stem diameter to the nearest cm., [nutrients.csv] Nutrient content was only assessed for the fertilization experiment and for the needles produced in 2010. Needle samples were taken in 14 October 2010. Nitrogen concentration in needles was determined in a LECO elemental analyzer (model CHN 628) while phosphorus was analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES) using a Perkin-Elmer Optima 4300DV (Massachusetts, USA) at the central laboratory facilities of Universidade de Vigo – CACTI (www.uvigo.es/webs/cactiweb/). Nitrogen and P concentration were expressed in mg g-1 tissue on a dry weight basis., [phenols.csv] See “resin.csv” below, for details on the sampling protocol for defensive compounds estimation. Polyphenolics were extracted from a subsample of 30 mg of needles or phloem, by immersion in a metanol-water solution (1:1 volume) in an ultrasonic bath of 15 min, followed by centrifugation and extract dilution. Colorimetric measurements were taken with a Biorad 650 microplate reader (Bio-Rad Laboratories, Philadelphia, PA,USA) a 740 nm., [pissodes.csv] During the summer of 2011 both experiments suffered an outbreak of Pissodes castaneus. We assessed the abundance of the damage caused by this curculionid in each ramet on 16 September 2011, by visually inspecting the scars produced in the main stem and assigning them to a four category ordinal scale (none, rare, abundant and very abundant)., [reproduction.csv] Monitoring of ramet reproduction comprised measures of reproductive effort (number of female strobili and abundance of male strobili) from 2010 to 2013, and measures of reproductive output (number of ripe cones, total number of seeds produced and number and dry weight of healthy seeds) from 2012 to 2015. Strobili cohorts blooming in 2010 to 2013 were hence monitored to ripeness (in 2012 to 2015). All female flowering strobili were visually counted and ripe cones collected, while male strobili shoots were counted and their length visually estimated to the nearest 5 mm to get an estimate of male strobili abundance. Pine cones where air-dried for several weeks and then oven-dried for seven days to induce opening (35ºC). Empty and healthy seeds were discriminated by decanting them on cold water, where empty seeds remain floating on the surface. After separating healthy and empty seeds for each cone, they were oven-dried again at 35ºC before weighing them to the nearest 1 mg with a precision scale., [resin.csv] On 14 October 2010 we collected needle samples from both experiments, to assess defensive compound (non-volatile resin and total polyphenolics) and nutrient content (nitrogen and phosphorus concentration). Both needles produced in 2009 and 2010 were collected (and analysed) separately in each ramet. Nutrient content was only assessed for the fertilization experiment and for the needles produced in 2010. Defensive compounds were also determined in phloem samples collected from all ramets on 16 april 2011 but due to logistical reasons, resin content data for phloem samples in 2010 were lost. Needle and phloem sampling was hence repeated at the end of the experiment, in 2013, in order to estimate non-volatile resin concentration. Total non-volatile resin content in phloem and needle tissue was determined by gravimetric analysis. Needle and phloem fresh samples (aprox 2-5 g) were immediately covered with hexane within pre-weighed test tubes, and sonicated for 20 min at 20ºC. Resin extraction continued for 24 h at room temperature under the fume hood and then the content of the tube was filtered with GFF filters (Whatman GF⁄ D, Whatman Int. Ltd, Maidstone, Kent, UK) into a second pre-weighed tube. The whole extraction procedure was repeated with each sample and the liquid fraction from both extractions pooled before letting them dry under the fume hood. Completely dried tubes were weighed with a precision scale to the nearest 0.0001 g. The solid fraction was oven-dried at 65ºC until constant weight. Total non-volatile resin content was estimated as mg of dried extracted resin per g of dry sample. Needle and phloem samples were analysed to estimate phenol content by the Folin-Ciocalteu method (Scalbert 1992), a colorimetric protocol using the Folin-Ciocalteu reagent and tannic acid as standard. Fresh samples were immediately oven-dried at 45ºC and manually ground in a mortar with liquid nitrogen., This data were collected with the support of the Spanish Goverment (grant numbers AGL2012-40151-C03-01, AGL2015-68274-C3-2-R)., growth.csv, nutrients.csv, phenols.csv, pissode.csv, reproduction.csv, resin.csv, Peer reviewed

DOI: http://hdl.handle.net/10261/311371
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311371
HANDLE: http://hdl.handle.net/10261/311371
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311371
PMID: http://hdl.handle.net/10261/311371
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311371
Ver en: http://hdl.handle.net/10261/311371
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311371

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311372
Dataset. 2022

TABLE_1_KNOCK-OUT OF CMNAC-NOR AFFECTS MELON CLIMACTERIC FRUIT RIPENING.XLSX

  • Liu, Bin
  • Santo Domingo, Miguel
  • Mayobre, Carlos
  • Martín-Hernández, Ana Montserrat
  • Pujol, Marta
  • García-Mas, Jordi
1 table. -- Supplementary Table 1: List of primers and their uses., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311372
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311372
HANDLE: http://hdl.handle.net/10261/311372
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311372
PMID: http://hdl.handle.net/10261/311372
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311372
Ver en: http://hdl.handle.net/10261/311372
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311372

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311375
Dataset. 2022

IMAGE_2_KNOCK-OUT OF CMNAC-NOR AFFECTS MELON CLIMACTERIC FRUIT RIPENING.TIF

  • Liu, Bin
  • Santo Domingo, Miguel
  • Mayobre, Carlos
  • Martín-Hernández, Ana Montserrat
  • Pujol, Marta
  • García-Mas, Jordi
1 figure., Fruit ripening is an important process that affects fruit quality. A QTL in melon, ETHQV6.3, involved in climacteric ripening regulation, has been found to be encoded by CmNAC-NOR, a homologue of the tomato NOR gene. To further investigate CmNAC-NOR function, we obtained two CRISPR/Cas9-mediated mutants (nor-3 and nor-1) in the climacteric Védrantais background. nor-3, containing a 3-bp deletion altering the NAC domain A, resulted in ~8 days delay in ripening without affecting fruit quality. In contrast, the 1-bp deletion in nor-1 resulted in a fully disrupted NAC domain, which completely blocked climacteric ripening. The nor-1 fruits did not produce ethylene, no abscission layer was formed and there was no external color change. Additionally, volatile components were dramatically altered, seeds were not well developed and flesh firmness was also altered. There was a delay in fruit ripening with the nor-1 allele in heterozygosis of ~20 days. Our results provide new information regarding the function of CmNAC-NOR in melon fruit ripening, suggesting that it is a potential target for modulating shelf life in commercial climacteric melon varieties., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311375
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311375
HANDLE: http://hdl.handle.net/10261/311375
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311375
PMID: http://hdl.handle.net/10261/311375
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311375
Ver en: http://hdl.handle.net/10261/311375
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311375

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311387
Dataset. 2022

SUPPLEMENTARY FILES OF THE ARTICLE "CRISPR/CAS9‐DIRECTED GENE TRAP CONSTITUTES A SELECTION SYSTEM FOR CORRECTED BCR/ABL LEUKEMIC CELLS IN CML" [DATASET]

  • Vuelta, Elena
  • Ordóñez, José Luis
  • Sanz, David J.
  • Ballesteros, Sandra
  • Hernández, Jesús M.
  • Méndez-Sánchez, Lucía
  • Sánchez-Martín, M.
  • García-Tuñón, Ignacio
The promoter‐less CRISPR‐Trap system efficiently the specific integration of the gene trap donor in BCR/ABL but is not a robust reporter system to select corrected cells. As a first approach, we generate a dsDNA donor by PCR amplification containing a SAT2A‐ Venus interfering cassette (Figure S1A). The K562 cell line was divided into three experimental groups according to the conditions for their subsequent electroporation: a) with the donor dsDNA (donor), b) with the donor DNA and Cas9 nuclease without sgRNA (Cas9 + donor) and c) with the donor DNA, Cas9 nuclease and the specific BCR/ABL sgRNA (CRISPR/Cas9 + donor). Twenty‐four hours after electroporation, no fluorescent cells (Venus + cells) were observed in any of the three groups (Figure S1B). The result of the site‐unspecific PCR (In 5´F/Venus R, Table 1) which amplify a region contained entirely in the donor HDR dsDNA, showed a band of the expected size in the Cas9 + donor and CRISPR/Cas9 + donor groups, but not in the donor group (Figure S1C), implying that all donor HDR dsDNA not integrated in the genome was fully degraded. In contrast, the sitespecific PCR (Out 5´F/Venus R, Table 1) corroborated the correct insertion of the donor HDR dsDNA at the BCR/ABL target sequence only in the CRISPR/Cas9 + donor group, with no sitespecific integration detected in any of the controls (Figure S1C). These results were corroborated by Sanger sequencing of the PCR products, supporting the HDR‐mediated insertion of the donor dsDNA into the CRISPR/Cas9 + donor group., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311387
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311387
HANDLE: http://hdl.handle.net/10261/311387
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311387
PMID: http://hdl.handle.net/10261/311387
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311387
Ver en: http://hdl.handle.net/10261/311387
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311387

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311433
Dataset. 2022

IMAGE_1_KNOCK-OUT OF CMNAC-NOR AFFECTS MELON CLIMACTERIC FRUIT RIPENING.TIF

  • Liu, Bin
  • Santo Domingo, Miguel
  • Mayobre, Carlos
  • Martín-Hernández, Ana Montserrat
  • Pujol, Marta
  • García-Mas, Jordi
1 figure., Fruit ripening is an important process that affects fruit quality. A QTL in melon, ETHQV6.3, involved in climacteric ripening regulation, has been found to be encoded by CmNAC-NOR, a homologue of the tomato NOR gene. To further investigate CmNAC-NOR function, we obtained two CRISPR/Cas9-mediated mutants (nor-3 and nor-1) in the climacteric Védrantais background. nor-3, containing a 3-bp deletion altering the NAC domain A, resulted in ~8 days delay in ripening without affecting fruit quality. In contrast, the 1-bp deletion in nor-1 resulted in a fully disrupted NAC domain, which completely blocked climacteric ripening. The nor-1 fruits did not produce ethylene, no abscission layer was formed and there was no external color change. Additionally, volatile components were dramatically altered, seeds were not well developed and flesh firmness was also altered. There was a delay in fruit ripening with the nor-1 allele in heterozygosis of ~20 days. Our results provide new information regarding the function of CmNAC-NOR in melon fruit ripening, suggesting that it is a potential target for modulating shelf life in commercial climacteric melon varieties., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311433
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311433
HANDLE: http://hdl.handle.net/10261/311433
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311433
PMID: http://hdl.handle.net/10261/311433
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311433
Ver en: http://hdl.handle.net/10261/311433
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311433

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311451
Dataset. 2022

DATASHEET1_THE EMERGENCE OF GENOME EDITING—INNOVATION NETWORK DYNAMICS OF ACADEMIC PUBLICATIONS, PATENTS, AND BUSINESS ACTIVITIES.DOCX

  • Laibach, Natalie
  • Bröring, Stefanie
Supplementary Table S1: Databases and corresponding search strings for data retrieval., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311451
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311451
HANDLE: http://hdl.handle.net/10261/311451
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311451
PMID: http://hdl.handle.net/10261/311451
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311451
Ver en: http://hdl.handle.net/10261/311451
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311451

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311458
Dataset. 2022

TABLE_4_THE LACK OF ALTERNATIVE OXIDASE 1A RESTRICTS IN VIVO RESPIRATORY ACTIVITY AND STRESS-RELATED METABOLISM FOR LEAF OSMOPROTECTION AND REDOX BALANCING UNDER SUDDEN ACUTE WATER AND SALT STRESS IN ARABIDOPSIS THALIANA.DOCX

  • Saz, Néstor F. del
  • Iglesias-Sanchez, Ariadna
  • Alonso-Forn, David
  • López-Gómez, Miguel
  • Palma, Francisco
  • Clemente-Moreno, María José
  • Fernie, Alisdair R.
  • Ribas-Carbó, Miquel
  • Florez-Sarasa, Igor
1 table. -- Supplemental Table 4. Primers used in the qPCR analyses performed in this study., In plants salt and water stress result in an induction of respiration and accumulation of stress-related metabolites (SRMs) with osmoregulation and osmoprotection functions that benefit photosynthesis. The synthesis of SRMs may depend on an active respiratory metabolism, which can be restricted under stress by the inhibition of the cytochrome oxidase pathway (COP), thus causing an increase in the reduction level of the ubiquinone pool. However, the activity of the alternative oxidase pathway (AOP) is thought to prevent this from occurring while at the same time, dissipates excess of reducing power from the chloroplast and thereby improves photosynthetic performance. The present research is based on the hypothesis that the accumulation of SRMs under osmotic stress will be affected by changes in folial AOP activity. To test this, the oxygen isotope-fractionation technique was used to study the in vivo respiratory activities of COP and AOP in leaves of wild-type Arabidopsis thaliana plants and of aox1a mutants under sudden acute stress conditions induced by mannitol and salt treatments. Levels of leaf primary metabolites and transcripts of respiratory-related proteins were also determined in parallel to photosynthetic analyses. The lack of in vivo AOP response in the aox1a mutants coincided with a lower leaf relative water content and a decreased accumulation of crucial osmoregulators. Additionally, levels of oxidative stress-related metabolites and transcripts encoding alternative respiratory components were increased. Coordinated changes in metabolite levels, respiratory activities and photosynthetic performance highlight the contribution of the AOP in providing flexibility to carbon metabolism for the accumulation of SRMs., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311458
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311458
HANDLE: http://hdl.handle.net/10261/311458
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311458
PMID: http://hdl.handle.net/10261/311458
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311458
Ver en: http://hdl.handle.net/10261/311458
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311458

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311464
Dataset. 2022

TABLE_3_THE LACK OF ALTERNATIVE OXIDASE 1A RESTRICTS IN VIVO RESPIRATORY ACTIVITY AND STRESS-RELATED METABOLISM FOR LEAF OSMOPROTECTION AND REDOX BALANCING UNDER SUDDEN ACUTE WATER AND SALT STRESS IN ARABIDOPSIS THALIANA.DOCX

  • Saz, Néstor F. del
  • Iglesias-Sanchez, Ariadna
  • Alonso-Forn, David
  • López-Gómez, Miguel
  • Palma, Francisco
  • Clemente-Moreno, María José
  • Fernie, Alisdair R.
  • Ribas-Carbó, Miquel
  • Florez-Sarasa, Igor
Supplemental Table 3. Absolute metabolite levels in leaves of WT and AOX1a plants under control conditions and after 1 day of severe (300 mM) NaCl and Mannitol treatments as measured by GC-MS (see material and methods for details). Data is presented as means ± SE for 4 to 6 biological replicates. Bold numbers denote significant differences (P < 0.05) to the control condition in each genotype separately. Asterisks denote significant differences (P < 0.05) between WT and aox1a plants in each experimental condition. †Denotes metabolites detected only in one replicate in WT at control conditions. ‘n.d.’ denotes cases for not detected metabolites., In plants salt and water stress result in an induction of respiration and accumulation of stress-related metabolites (SRMs) with osmoregulation and osmoprotection functions that benefit photosynthesis. The synthesis of SRMs may depend on an active respiratory metabolism, which can be restricted under stress by the inhibition of the cytochrome oxidase pathway (COP), thus causing an increase in the reduction level of the ubiquinone pool. However, the activity of the alternative oxidase pathway (AOP) is thought to prevent this from occurring while at the same time, dissipates excess of reducing power from the chloroplast and thereby improves photosynthetic performance. The present research is based on the hypothesis that the accumulation of SRMs under osmotic stress will be affected by changes in folial AOP activity. To test this, the oxygen isotope-fractionation technique was used to study the in vivo respiratory activities of COP and AOP in leaves of wild-type Arabidopsis thaliana plants and of aox1a mutants under sudden acute stress conditions induced by mannitol and salt treatments. Levels of leaf primary metabolites and transcripts of respiratory-related proteins were also determined in parallel to photosynthetic analyses. The lack of in vivo AOP response in the aox1a mutants coincided with a lower leaf relative water content and a decreased accumulation of crucial osmoregulators. Additionally, levels of oxidative stress-related metabolites and transcripts encoding alternative respiratory components were increased. Coordinated changes in metabolite levels, respiratory activities and photosynthetic performance highlight the contribution of the AOP in providing flexibility to carbon metabolism for the accumulation of SRMs., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311464
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311464
HANDLE: http://hdl.handle.net/10261/311464
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311464
PMID: http://hdl.handle.net/10261/311464
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311464
Ver en: http://hdl.handle.net/10261/311464
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oai:digital.csic.es:10261/311464

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311468
Dataset. 2022

SUPPLEMENTARY FILES OF THE ARTICLE &QUOT;DECIPHERING BIOMARKERS FOR LEPTOMENINGEAL METASTASIS IN MALIGNANT HEMOPATHIES (LYMPHOMA/LEUKEMIA) PATIENTS BY COMPREHENSIVE MULTIPRONGED PROTEOMICS CHARACTERIZATION OF CEREBROSPINAL FLUID&QUOT; [DATASET]

  • Juanes-Velasco, Pablo
  • Galicia, N.
  • Pin, Elisa
  • Jara-Acevedo, Ricardo
  • Carabias-Sánchez, Javier
  • García-Valiente, R.
  • Lécrevisse, Quentin
  • Pedreira, C. E.
  • Góngora, Rafael
  • Sanchez-Santos, Jose Manuel
  • Lorenzo-Gil, Héctor
  • Landeira-Viñuela, Alicia
  • Bareke, Halin
  • Orfao, Alberto
  • Nilsson, Peter
  • Fuentes, Manuel
The following are available online at https://www.mdpi.com/article/10.3390/cancers14020449/s1. Supplementary Figures. Figure S1: Distribution of pathological CSF samples (without healthy ones) among each phase of study and the different groups according to the incidence of the pathology, depending on the infiltration (CSF +/− LM) and the primary tumor (hematologic and solid tumor). Figure S2: Quality control images of the planar protein microarrays generated. Figure S3: A quantile normalization in planar protein microarrays. Figure S4: Coomasie gels which indicate protein distribution across samples. Figure S5: Venn diagrams of total identified proteins with LC-MS/MS. Figure S6: Plots showing the functional proteins using the Reactome for different conditions. Figure S7: Differential protein profiles within CSF + LM according to primary tumor (Lymphoma) by protein microarrays. Figure S8: Differential protein profiles within CSF + LM according to primary tumor (Leukemia) by protein microarrays. Figure S9: Differential protein profiles within CSF + LM according to primary tumor (Lymphoma) by affinity proteomics. Figure S10: Differential protein profiles within CSF + LM according to primary tumor (Leukemia) by affinity proteomics. Figure S11: Summary of the multipronged proteomics characterization among the different phases of study. Supplementary Tables. Table S1: Table of clinical-biological characteristics from the whole CSF samples used in the study. Table S2: Antibodies list used in Planar Protein Microarrays. Table S3: Antibodies list used in Beads Suspension Microarrays. Table S4: Protein identification with LC-MS/MS among the different strategies and their emPAI quantification. Table S5: Boxplots of the protein identified in validation and confirmation phases, respectively, comparing the different groups of study. Table S6: Intensity data results from Planar Protein Microarrays. Table S7: Intensity data results from Beads Suspension Microarrays. Table S8: ROC analysis list of potential biomarker panel on CSF +/− LM and the different comparisons by protein arrays and affinity proteomics., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311468
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311468
HANDLE: http://hdl.handle.net/10261/311468
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311468
PMID: http://hdl.handle.net/10261/311468
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311468
Ver en: http://hdl.handle.net/10261/311468
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311468

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