BUSCADOR RECOLECTA

The supporting information: Figure S1: Freeze-dried samples obtained from the cultivated mushrooms and employed in the analysis; Table S1: Percentage of the most-abundant VOCs detected in the freeze-dried samples by the optimized method based on SPME fiber extraction and GC-MS analysis., Peer reviewed

The Supplementary Material: Supplementary Fig 2 FiN.tif Supplementary Fig 3 FiN.tif Supplementary Table 1 Oligos & Probes.docx Supplementary Table 2 Patients.xlsx Supplementary Table 3 TLDAs.xlsx Supplementary Table 4 PBMC analysis.xlsx Supplementary Fig1 FiN.tif, Peer reviewed

The supporting information: Figure S1: Full MS spectra and structures of coumarins compounds. A: 2-nor-1,2-secolycoserone, B: Lycoserone, C: 6,11′-dihydroxy-1′ H-lycoserone, and D: 9-reduced-10′,11′-dehydro-1′-H-lycoserone, Figure S2: 1H NMR experiment for compound 1, Figure S3: 13C NMR experiment for compound 1, Figure S4: DEPT 13C NMR experiment for compound 1, Figure S5: 1H NMR experiment for compound 2, Figure S6: 13C NMR experiment for compound 2, Figure S7: DEPT 13C NMR experiment for compound 2, Figure S8: Molecular structure of the 2-nor-1,2-secolycoserone and ent-labda-8,13-E-diene-15-ol, Table S1: 1H NMR (500 MHz, CDCl3) experimental data for compound 1, Table S2: 13C NMR (126 MHz, CDCl3) experimental data for compound 1, Table S3: 1H NMR (500 MHz, CDCl3) experimental data for compound 2, Table S4: 13C NMR (126 MHz, CDCl3) experimental data for compound 2, Video S1: Live-cell imaging of HeLa cells exposed to compound 1 (80 µM), Video S2: Live-cell imaging of HeLa cells exposed to compound 2 (50 µM)., Peer reviewed

Supplementary data 1., Peer reviewed

Figure S1. Evaluation of the preference before and after knowing the percentage of sucrose of each sample (46 panellists). Table S1. Formulations (% w/w) used for the manufacturing of low sucrose strawberry jams with sunflower pectin. Table S2. p values obtained after penalty analysis for the JAR questions (n = 46 assessors)., Peer reviewed

Supplementary data 1: Figure S1. MALDI-TOF-MS profiling of a) milk and milk-colorant; b) casein and casein-colorant; c) whey and whey-colorant (5000–30,000 Da). Figure S2. Reaction of genipin and glycine to form the monomer and since two monomers, the dimer formation., Peer reviewed

Data from the extensive literature review on each protein family and their allergenic members is fully described/summarised in the Electronic supplementary material section, as an excel file. This excel file is divided into eight pages, each dedicated to a single protein family (2S albumins, nsLTP, ATI, cereal prolamins, profilins, legumins, vicilins and PR-10 proteins). All the abbreviations used in the excel file are presented in this manuscript as an abbreviation list. Based on the information gathered in this excel file, Table 3 was constructed, thus summarising the data presented in the Electronic supplementary material section., Peer reviewed

[Methods] Methodology appears in detail in DOI: 10.1242/jeb.243342. The data set contains data on genotypes in SERT SNP290 and on three experiments on coping style on free living Great tits Parus major: The first experiment was risk-taking behavior in reaction to a flag that was attached to the top of the nest box at the time of feeding of the nestlings. The method was previously used and tested by Cole and Quinn (2014), and reflects the proactivity of the birds in front of a new object. Time to enter into the flagged nest box correlated with additional assays with the same birds in captivity, in which exploration rate in a novel environment was measured. This novel environment assay additionally stresses the trade-off between risk-taking and chick provisioning. This experiment was conducted when the chicks had a median age of 9 days (range 9 - 12) by video recording at the distance of 7-36 meters from the nests (median= 16 m), depending on the presence of vegetation around each nest. Distance from the observer to the nest was set as a covariate in the analysis. To ascertain for the effect of the flag (novel object) in our experiment, we first recorded the normal latency of the birds to enter the nest-box without any flag. Then, we recorded the behavior of the birds with the flag attached at the top of the nest-box. We recorded the behavior of the birds with a video camera, and corrected for the distance of the camera from the nest, since this could affect to the time of appearance of the bird in the video. The flag was removed after 40 minutes. This behaviour was tested in 34 individuals, using the birds that entered the nestbox during the control experiment (with no flag). However, when considering only the birds that entered the nestbox during the flag trial, sample size was reduced 24 birds. The trait has been shown previously to be repeatable (Cole and Quinn, 2014). The second test focused on distress calling rate during handling, which has also been previously recognized as a rate of proactivity (boldness): in black-capped chickadees (Poecile atricapillus), distress calling rate was positively related to exploration rate, which is one of the main characteristics of the proactive coping style (Guillette and Sturdy, 2011). In siskins (Carduelis spinus), individuals uttering more often distress calls also displayed bolder behaviors in front of a novel object (Mateos-González and Senar, 2012; Pascual and Senar, 2014). Distress calling has therefore been used as a proxy of proactivity in several studies (Andersen, 2012; Pascual and Senar, 2014; Richardson et al., 2016; Senar et al., 2017; Thorsteinsen, 2015). This was tested during the capture of the parents, normally at age 15 days of the chicks. Captured animals were kept in a ringing bag for a period of about 5 min to calm down. Just after extracting the bird from the ringing bag, distress calling rate was quantified by counting the number vocalized distress calls that were emitted by the birds during the next 15s of handling while holding their legs and moving a straight finger positioned at 1-2 cm from the beak of the focal bird (Markó et al., 2013; Senar et al., 2017). We recorded this behavior on a total of 64 birds. The trait has been shown previously to be repeatable (Senar et al., 2017). The third test focused on the propensity of incubating females to utter hissing calls towards a nest predator. This behaviour was first described in Great tits by Krams et al. (2014). We measured the hissing response of incubating females to a mouse model meanwhile we inserted the model head into the entrance of the nest box. The head of the mouse was kept in this position for 15 s. We counted the number of calls of incubating females in that period. As soon as 15 s was over, the mouse was removed and the observer silently moved away. The method has been used in several other papers although using a woodpecker as a nest predator (Krams et al., 2014; Thys et al., 2021; Timm et al., 2019). We recorded this behavior on a total of 34 females. The trait has been found previously to be highly repeatable (Koosa and Tilgar, 2016; Krams et al., 2014). DNA was extracted from blood samples using Ecogen MasterPure DNA purification kit (MCD85201). Primers were designed using the great tit genome (assembly 1.3, accession number: SRS1185780, (Laine et al., 2016) blasting with the reverse complement of the SERT promoter interval of the great tit (GenBank: accession number KP869099) (Riyahi et al., 2015). The primer sequences were as follows: Forward primer 5´-TTCAGCAATGCACAAAGTCCAG-3´, Reverse primer 5´-ACTCCAGGTCTCCCTGTCCTC-3´. Genetic polymorphisms within the SERT loci were genotyped by standard PCR amplification and direct sequencing of the resulting amplicon. 50 ng genomic DNA was amplified in 25 µl reactions containing 10x reaction buffer. All the samples were sequenced at the Genomics Core Facility of the University of Pompeu Fabra (Barcelona, Spain) using an Applied Biosystems sequencing platform. All of the sequences were aligned using Sequencher v4.6 (Gene Codes Corporation, MI, Ann Arbor, USA) and visually checked for polymorphisms (SNPs). The names of the SNPs were given by the position of each SNP in the resulting amplicon. [Usage notes] We intended to conduct the three behavioral experiments in our breeding pairs in all of the nest boxes. However, the number of individuals involved in each test varied because we were unable to perform some tests on some nests because the chicks died before conducting the experiment or because the parents abandoned the nests or were not captured. This is why sample sizes for the different experiments differ., The coping style of an individual in relation to potentially dangerous situations has been suggested to be inherited in a polygenic fashion, being SERT one of the candidate genes. In this paper, we assessed in free-living great tits Parus major the association between SNP290 in the SERT promoter and three standard fear-related behaviors, namely the response of the birds to a black and white flag fixed to the top of the nest-box, distress calling rate of the birds in the hand once captured and the hissing call of incubating females when approached by a predator. We found a strong association between SNP290 polymorphism and the three risk-taking behaviors, with birds with genotype CT entering faster to the nest box with the flag and displaying more distress calls and less hissing calls. CT birds could therefore be described as more proactive than CC individuals. These results also suggest that hissing behavior should be regarded as a fear-induced shy behavior, and confirm that SERT has an important function in relation to risk aversion behaviors and coping style., Ministerio de Economía y Competitividad, Award: CGL-2016-79568-C3-3-P; Ministerio de Ciencia e Innovación, Award: CGL-2020 PID2020-114907GB-C21., Peer reviewed

Fridericia chica (Bonpl.) L.G. Lohmann (synonym Arrabidaea chica Verlot) (Bignoniaceae) is widely used in folk medicine in Brazil for health benefits. Considering overcoming pitfalls of scaling up production of plant extracts, herein the effects of N2 atomizing gas in spray-drying process of standardized plant extract production is reported. Samples were monitored by in vitro antioxidant activity and microbiological evaluation. The type of gas used did not interfere in the product’s color. However, the drying atmosphere influenced 3-deoxyanthocyanines content when using air as atomizing gas, decreasing carajurin (37.5%) content with concomitant increase in luteolin yield (24.1%). Both drying processes preserved the samples’ pharmacological profile. The antioxidant activity was also similar IC50 (445.60 ± 12.73 and 466.99 ± 46.11). In the cell migration test with HaCaT cells, the extract dried under air flow (5 μg/mL) promoted wound closure by 78% (12 hours) whereas the extract dried using N2 flow promoted 49% (12 hours), with 98% closure (12 hours) for the positive control. The antimicrobial evaluation for Staphylococcus aureus did not differ with the different drying atmospheres, with MIC (minimum inhibitory concentration) at 0.39 mg/mL. Therefore, the drying process reported herein did not interfere with the outcome of the biological activity., Peer reviewed

[Methods] Distribution data was collected manually from Reptile Database (https://reptile-database.reptarium.cz/), and the biogeographic analyses were entirely performed in the R framework. [Usage notes] Information about directory structure can be found in the README.md file., The geographic distribution of biodiversity is central to understanding evolutionary biology. Paleogeographic and paleoclimatic histories often help to explain how biogeographic patterns unfold through time. However, such patterns are also influenced by a variety of other factors, such as lineage diversification, that may affect the probability of certain types of biogeographic events. The complex and well-known geologic and climatic history of Afro-Arabia, together with the extensive research on reptile systematics in the region, makes Afro-Arabian squamate communities an ideal system to investigate biogeographic patterns and their drivers. Here we reconstruct the phylogenetic relationships and the ancestral geographic distributions of several Afro-Arabian reptile clades (totaling 430 species) to estimate the number of dispersal, vicariance and range contraction events. We then compare the observed biogeographic history to a distribution of simulated biogeographic events based on the empirical phylogeny and the best-fit model. This allows us to identify periods in the past where the observed biogeographic history was likely shaped by forces beyond the ones included in the model. We find an increase in vicariance following the Oligocene, most likely caused by the fragmentation of the Afro-Arabian plate. In contrast, we did not find differences between observed and expected dispersal and range contraction levels. This is consistent with diversification enhanced by environmental processes and with the establishment of a dispersal corridor connecting Africa, Arabia and Eurasia since the middle Miocene. Finally, here we show that our novel approach is useful to pinpoint events in the evolutionary history of lineages that might reflect external forces not predicted by the underlying biogeographic model., Peer reviewed