BUSCADOR RECOLECTA

(a, b) Heads from GMR>+ (a) and GMR>Upd (b) adult males; frontal view. While the median eye size (rE: c) of GMR>Upd flies is approximately 1.4 times that of control (GMR>+) ones (statistically significant), the fluctuating asymmetry index (“precision”) (FAi: d) is indistinguishable between the 2 genotypes. Female (F) and male (M) distributions are shown. (e, f) Eye primordia stained for the apoptotic marker Dcp-1. Apoptotic signal is observed in both GMR>Upd as well as in GMR>+ control primordia. The data used in the graphs shown in the figure can be found in “S1_Fig 4_data” in the Supporting information file S1 Raw Data., Peer reviewed

(a, b) Representative late L3 eye imaginal discs, stained for the mitotic marker phosphor-histone H3 (PH3), and counterstained with rhodamine phalloidin (“actin”). (c) Distribution of mitotic rate, measured as the rate of PH3-positive area relative to the % of available area (nonapoptotic) in the anterior domain (regions outlined in yellow) in the 2 genotypes (see S1 Statistical Methods). Bayes Factor (BF) value close to 0 allows accepting the null hypothesis: “There is no significant differences between genotypes.” BF = 0.37. The bar indicates an 80% power to detect differences. This analysis indicates that mitotic rate of progenitors does not change in optix>tkvRI relative to optix>+ controls (see S1 Statistical Methods). The data used in the graphs shown in the figure can be found in “S1_Fig 2_data_A” and “S1_Fig 2_data_B” in the Supporting information file S1 Raw Data., Peer reviewed

The intraindividual FA (FAi) is calculated using the left and right eyes from the same individual. The interindividual FA (FAn) is calculated as the difference between the left eye of an individual and the right eye randomly chosen from the population of measured eyes. The difference between FAn and FAi was computed for each fly (ΔFA). If there were no “individual” effect, ΔFA should be 0. The effect of each genotype on ΔFA median, their p.values, and the predicted median for each of them are shown (see S1 Statistical Methods). The ΔFA median is slightly, though significantly greater than 0 across all genotypes relative to the control (optix>+). This result indicates that eye size within an individual is slightly correlated irrespective of genotype. The data used in the graphs shown in the figure can be found in “Fig 1_S6Fig 1_data” in the Supporting information file S1 Raw Data., Peer reviewed

L3 dpp-Z discs (a, b), stained for apoptosis (dcp-1, green), dpp transcription (beta-galactosidase, red), and the retinal differentiation marker Elav (blue). (a) Control (“+”: Oregon-R) and (b) Bar mutant discs. The ellipse approximately outlines the eye region. “a” marks the antennal primordium., Peer reviewed

Results are shown for rE and sFAi of eyes from individuals of the following genotypes: “+”: Oregon-R (Or-R) wild-type strain; “optix>+”: Progeny from the cross optix-GAL4 to Or-R; “optix>UAS-CherryRI”: The optix-GAL4; UAS-Cherry_RNAi progeny obtained by crossing optix-GAL4 to UAS-Cherry_RNAi. This dataset was obtained independently of the datasets included in Fig 2 of the main text. The median rE of optix>CherryRI eyes is slightly higher than that of the optix>+ reference control group; however, it is not significantly higher than that of the wild-type Or-R group (a). There are no significant differences in sFAi between any of the groups compared (b). The table shows the statistical analysis of rE and sFAi among the different groups considered. This analysis concludes that there is no significant effect of driving a nonspecific, neutral RNAi on eye size precision and that the effect on final eye size is within the variability present among the alternative control lines used (An equivalent table is explained in S2 Table. Further details on the statistical analysis can be found in the main text Materials and methods section). The data underlying the graphs shown in the figure can be found in “S3_Fig 1_data” in the Supporting information file S1 Raw Data., Peer reviewed

(a, a’) optix>GFP primordium stained for GFP, the photoreceptor marker Elav and Cas3*. The expression of the optix-GAL4 driver, as detected by GFP-expression is outlined (in a’). The Cas3* signal is low (a’). (b, b’) optix>+ primordium stained for the G2-marker Cyclin B (CycB) and Dcp-1. Dcp-1 signal is detected in a band of cells anterior to the differentiating wavefront (this latter lacks CycB expression)., Peer reviewed

Additional file 1: Figure S1. A minimal increase in Cdc20Slp1 levels causes sensitivity to MBC and defective mitotic arrest. A. Slp1 protein level was determined from asynchronously growing cells of the indicated genotypes. Cdc2 was used as a loading control. B. Spot growth assay of the indicated strains and condition. C-E. Representative examples of mitotic arrest assay of the indicated strains. Cell cultures were grown at permissive temperature (32ºC) and then shifted to restrictive temperature (18ºC). Samples were taken every hour and the septation index calculated form more than 200 cells in each time point and condition. Representative examples of two independent experiments are shown., Ministerio de Economía y Competitividad Ministerio de Ciencia e Innovación Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía Fundación Séneca de la Región de Murcia National Institutes of Health, Peer reviewed

The dataset contains 3 files.-- Information on the methodology and quality control can be found in the files description and references included in the README_NWIberian_DO_1969_2018_V02.txt file, The dataset NWIberian_DO_1969_2018_V02 contains: averaged yearly data from compiled datasets of dissolved oxygen concentration, temperature and salinity at the 27.1 isopycnal level from different cruises between 1969 and 2018, and covering the area 40°- 43°N and 9°-11°W; time series of the potential drivers of the dissolved oxygen change in the region of ENACW formation (45°- 49°N and 9°-20°W) and off the NW Iberian coast (40°- 43°N and 9°-11°W); distributions of sea surface temperature over the area 39°- 55°N and 6°-30°W and averaged over differente time periods; and table with examples from other upwelling regions in terms of concentration and trends of dissolved oxygen, Funding for this dataset was provided by the Spanish Ministry of Science and Innovation through a Personal Técnico de Apoyo grant (PTA2019-017492-I) and the CAPTA project (CAPTA 0062_CAPTA_1_E, POCTEP 2021-2027), ENACWsptseries19692018V02.csv.-- SST_NAtlantic.csv.-- Oxygen_upwelling_systems.csv.-- README_NWIberian_DO_1969_2018_V02.txt, No

Results of the beta regression, assessing the effect of NewAlters on the Abstract Similarity Score., Peer reviewed

Descriptive statistics for the main variables employed in the beta regression analysis., Peer reviewed