Resultados totales (Incluyendo duplicados): 34260
Encontrada(s) 3426 página(s)
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311088
Dataset. 2022

EFFECT OF MUTATING THE SAUR63 CLADE

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
A) Genomic map showing positions of genes and locations of mutations from CRISPR/Cas9 mutagenesis in the 9x-saur mutant based on the TAIR10 Arabidopsis genome annotation. The first sgRNA directed cuts in both SAUR61 and SAUR64 (green arrows), creating a deletion between them (green bar) and leaving behind a hybrid gene with a frameshift at the junction site (symbolized by a green X). The second sgRNA directed cuts in the remaining genes (blue arrows, with lighter blue indicating slight mismatches between the sgRNA and the genome), leading to deletions (blue bars) and/or frameshift mutations (blue X’s). SAUR gene names are abbreviated as S61 etc. SAUR61-SAUR68 are on chromosome 1 and SAUR75 is on chromosome 5. B,C) 5-day-old seedlings grown on 1x MS/1% Suc medium in long days. Scale bar, 1 mm. D) Hypocotyl lengths of seedlings grown for 4d in short days on 0.5x MS medium. n, 27 (wild type), 22 (9x-saur). E) Cotyledon area of seedlings grown on vertically oriented plates for 6d on MS/1% Suc medium. n, 16 (wild type), 22 (9x-saur). Graphs show means ± s.d. No statistical differences were detected between wild-type and 9x-saur mutant measurements by t-test. F) Sequences of guide RNAs used for CRISPR/Cas9-mediated mutagenesis, wild-type genes, and mutant alleles present in the 9x-saur mutant. Underlines indicate PAM motif adjacent to guide RNA target site, and any mismatches to the guide RNA sequence. Uppercase bold letters indicate insertion mutations. All alleles create frameshift mutations except for saur75-1, which has an in-frame deletion of 13 amino acids in the SAUR domain., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311088
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311088
HANDLE: http://hdl.handle.net/10261/311088
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311088
PMID: http://hdl.handle.net/10261/311088
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311088
Ver en: http://hdl.handle.net/10261/311088
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oai:digital.csic.es:10261/311088

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311089
Dataset. 2022

SUPPORTING INFORMATION FOR METABOLITE PROFILING OF SOY BY-PRODUCTS: A COMPREHENSIVE APPROACH

  • Sanchez Bragagnolo, Felipe
  • Álvarez-Rivera, Gerardo
  • Breitkreitz, Márcia Cristina
  • Ibáñez, Elena
  • Cifuentes, Alejandro
  • Soleo Funari, Cristiano
Response surfaces and contour plots of all optimized methods (Figures S1–S3) and GC-QToF-MS chromatogram of the individual soy by-products (Figure S4)., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311089
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311089
HANDLE: http://hdl.handle.net/10261/311089
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311089
PMID: http://hdl.handle.net/10261/311089
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311089
Ver en: http://hdl.handle.net/10261/311089
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311089

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311091
Dataset. 2022

HYPOCOTYL EPIDERMAL CELL SIZES

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
A-F) Each point represents the length (A, C, E) or width (B, D, F) of a single epidermal cell against distance of that cell from the base of the hypocotyl (x-axis). Seedlings were grown in short days for 2 (A, B), 3 (C, D) or 4 (E, F) days. Cells were measured from confocal z-stack projections using ML1:RFP PM-localized fluorescence (examples in S3G, S3H and S3I Fig). Data are from 3 cell files per seedling, for two seedlings per time point, except that wild type at 4 days and ost2-2 at 3 days data are from just one seedling each. In a replicate experiment with 2-day-old seedlings only, P35S:SAUR63:YFP:HA hypocotyls also had a greater proportion of longer cells than did wild-type hypocotyls., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311091
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311091
HANDLE: http://hdl.handle.net/10261/311091
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311091
PMID: http://hdl.handle.net/10261/311091
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311091
Ver en: http://hdl.handle.net/10261/311091
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311091

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311093
Dataset. 2022

ULTRASENSITIVE DETECTION OF SOY TRACES BY IMMUNOSENSING OF GLYCININ AND Β-CONGLYCININ AT DISPOSABLE ELECTROCHEMICAL PLATFORMS: APPENDIX A. SUPPLEMENTARY DATA

  • Blázquez-García, Marina
  • Arévalo, Beatriz
  • Serafín, Verónica
  • Benedé, Sara
  • Mata, Luis
  • Galán-Malo, Patricia
  • Segura-Gil, Isabel
  • Pérez, María Dolores
  • Pingarrón, José Manuel
Multimedia component 1., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311093
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311093
HANDLE: http://hdl.handle.net/10261/311093
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311093
PMID: http://hdl.handle.net/10261/311093
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311093
Ver en: http://hdl.handle.net/10261/311093
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311093

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311097
Dataset. 2022

SEEDLING PHENOTYPES OF PLANTS EXPRESSING SAUR63:X FUSION PROTEINS

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
A,B) Hypocotyl length (A) and hypocotyl tortuosity index [B, 1 –(distance between ends)/(contour length)]) of seedlings of indicated genotypes grown for 3d in darkness on plates with 0.5x MS medium. C,D) Root length (C) and root tortuosity index (D) of seedlings of indicated genotypes grown for 4d in long days on plates with 1x MS 1% Sucrose medium. E,F) Root length (E) and root tortuosity index (F) of seedlings of indicated genotypes grown for 4d in short days on plates with 0.5x MS medium. Graphs show means ± s.d. Letters in graphs indicate statistical classes based on Tukey’s Honestly Significant Difference test. n from left to right: Panels A,B: 51, 24, 26, 28, 23, 25, 25; Panels C,D: 34, 17, 23, 14, 19, 15, 16; Panels E,F: 43, 21, 18, 17, 16, 15, 17. The same genotypes were measured in panels A-F, with genotype designations shown only in panels E and F. G,H,I) Hypocotyl epidermal cells of seedlings of indicated genotypes grown in short days for 2 days, visualized with the ML1:RFP shoot epidermis plasma membrane marker. Shown are z-stack confocal image projections of the near side of the hypocotyl. Scale bar, 0.1 mm. S2 Fig shows measurements of cell sizes in this experiment., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311097
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311097
HANDLE: http://hdl.handle.net/10261/311097
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311097
PMID: http://hdl.handle.net/10261/311097
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311097
Ver en: http://hdl.handle.net/10261/311097
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311097

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311100
Dataset. 2022

SUPPLEMENTARY MATERIAL. ANALYSIS OF ALKYLPHENOLS, BISPHENOLS AND ALKYLPHENOL ETHOXYLATES IN MICROBIAL-FERMENTED FUNCTIONAL BEVERAGES AND BOTTLED WATER: OPTIMIZATION OF A DISPERSIVE LIQUID-LIQUID MICROEXTRACTION PROTOCOL BASED ON NATURAL HYDROPHOBIC EUTECTIC SOLVENTS

  • Baute-Pérez, David
  • Santana-Mayor, Álvaro
  • Herrera-Herrera, Antonio V.
  • Socas-Rodríguez, Bárbara
  • Rodríguez-Delgado, Miguel A.
Appendix A. Supplementary data: Supplementary data 1., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311100
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311100
HANDLE: http://hdl.handle.net/10261/311100
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311100
PMID: http://hdl.handle.net/10261/311100
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311100
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Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311100

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311107
Dataset. 2022

SEEDLING PHENOTYPES OF PLANTS EXPRESSING SAUR63:YFP:HA VARIANTS

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
A) Cotyledon area of seedlings of indicated genotypes grown on vertically oriented plates for 7d in long days in the absence (open bars) or presence (filled bars) of 1% sucrose. B) Root length of seedlings of indicated genotypes grown on vertically oriented plates for 4d in long days in the absence (open bars) or presence (filled bars) of 1% sucrose. C,F) Hypocotyl lengths (E) and hypocotyl tortuosity index [F, 1 –(distance between ends)/(contour length)] of seedlings grown on vertically oriented plates for 3d in darkness without sucrose. D) Cotyledon areas of P35S:SAUR63 and P35S:CBL11-12:SAUR6326-142 seedlings grown for 6d on vertically oriented MS 1% Suc plates. E) Hypocotyl lengths of P35S:SAUR63 and P35S:CBL11-12:SAUR6326-142 seedlings grown for 4d on vertically oriented 0.5x MS plates. Panels D and E show data for the three homozygous single-locus P35S:SAUR63 lines that differed most from wild type among seven lines analyzed. Graphs show means ± s.d. Letters in graphs indicate statistical classes based on Tukey’s Honestly Significant Difference test. n, from left to right: panel A: 26, 20, 25, 15, 22, 23, 24, 20, 22, 23, 19, 19, 21, 19, 21, 20; panel B: 19, 16, 18, 14, 20, 20, 21, 16, 21, 17, 19, 16, 18, 15, 16, 17; panel D: 77, 18, 17, 17, 19, 20; panel E: 119, 25, 24, 27, 21, 24; panels C and F: 35, 36, 45, 43, 47, 38, 41, 47., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311107
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311107
HANDLE: http://hdl.handle.net/10261/311107
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311107
PMID: http://hdl.handle.net/10261/311107
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311107
Ver en: http://hdl.handle.net/10261/311107
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311107

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311113
Dataset. 2022

SUPPLEMENTARY INFORMATION - A STRUCTURALLY UNIQUE FUSOBACTERIUM NUCLEATUM TANNASE PROVIDES DETOXICANT ACTIVITY AGAINST GALLOTANNINS AND PATHOGEN RESISTANCE

  • Mancheño, Jose M.
  • Atondo, Estíbaliz
  • Tomás-Cortázar, Julen
  • Lavín, José Luis
  • Plaza-Vinuesa, Laura
  • Martín-Ruiz, Itziar
  • Barriales, Diego
  • Palacios, Ainhoa
  • Navo, Claudio D.
  • Sampedro, Leticia
  • Peña-Cearra, Ainize
  • Pascual-Itoiz, Miguel Ángel
  • Castelo, Janire
  • Carreras-González, Ana
  • Castellana, Donatello
  • Pellón, Aize
  • Delgado, Susana
  • Ruas-Madiedo, Patricia
  • Rivas, Blanca de las
  • Abecia, Leticia
  • Muñoz, Rosario
  • Jiménez-Osés, Gonzalo
  • Anguita, Juan
  • Rodríguez, Héctor
Fig. S1. Genetic context of the tannase gene (FN_0616) within the F. nucleatum subsp. nucleatum 25586 genome. Fig. S2. TanBFnn is monomeric in solution. Fig. S3. TanBFnn substrate specificity. Fig. S4. Biochemical properties of TanBFnn. Fig. S5. Amino acid sequence and active site comparisons between TanBFnn and TanALp. Fig. S6. Representative snapshots of three different 1 microsecond MD simulations of ethyl gallate (EG, in red) bound to TanBFnn. Fig. S7. Representation of MD simulations of methyl digallate (MDG, in red) bound to TanBFnn. Fig. S8. SPD interacts with TanBFnn. Fig. S9. Structure of spermidine and its N1 and N8-acetylated derivatives in their charged form at neutral pH. Fig. S10. Multiple sequence alignment of TanBFnn with homologs found within the Fusobacteriales, particularly, from the genus Leptotrichia: Leptotrichia sp. OH3620_COT-345 (WP_125237392.1); L. wadei (WP_146961278.1); L. massiliensis (WP_071123737.1); L. buccalis (WP_157859547.1). Fig. S11. Multiple sequence alignment of TanBFnn with homologs from bacterial genera that are prevalent in the oral cavity: Aggregatibacter (WP_006718097.1); Selenomonas (WP_051586482.1); Veillonella (WP_127058572.1); Mitsuokella (WP_118475533.1). Fig. S12. Multiple sequence alignment of TanBFnn with homologs from bacterial genera present in the human gut: Leptotrichia (WP_157859547.1); Veillonella (WP_127058572.1); Megasphaera (WP_044503365.1); Roseburia (WP_173884414.1); Bacteroides (WP_164733466.1); Parabacteroides (WP_172733427.1); Lachnoclostridium (WP_087219107.1); Fusicatenibacter (WP_173816499.1); Dorea (WP_161159564.1); Blautia (WP_095175021.1); Lactobacillaceae (WP_054397147.1); Sutterella (GenBank: RGU74189.1). Table S1 related to Figure 1. Species harboring orthologous tannase proteins., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311113
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311113
HANDLE: http://hdl.handle.net/10261/311113
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311113
PMID: http://hdl.handle.net/10261/311113
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311113
Ver en: http://hdl.handle.net/10261/311113
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oai:digital.csic.es:10261/311113

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311117
Dataset. 2022

EPISTASIS AMONG SAUR63 AND PP2C.D5 LINES, AND PHENOTYPES RELATED TO PM H+-ATPASE ACTIVITY

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
A) Appearance of genotypes used for crosses presented in A and in Fig 2C and 2D, grown for 6d in long days in the presence of sucrose. Scale bar, 3 mm. B) Hypocotyl lengths of seedlings of indicated genotypes grown for 4d in short days in the absence of sucrose. Plants measured were F1 progeny of crosses of transgenic lines with each other or with wild-type Columbia, and were hemizygous for the indicated transgene(s). Fig 2C shows a subset of this data. A replicate experiment gave similar results. C) Appearance of PEST:SAUR63:CerFP:HA and wild-type seedlings grown with estradiol and in the absence or presence of 15 mM LiCl. Seedlings were grown for 3d under control conditions, and then transferred to plates with estradiol and with or without 15 mM LiCl, and grown for an additional 3d before imaging. Dots mark positions of root tips at the time of transfer to estradiol plates. Scale bar, 5 mm. D) Root growth of indicated genotypes in the absence (open bars) or presence (closed bars) of 15 mM LiCl. Seedlings were grown without LiCl for 5d, transferred to plates containing 0 or 15 mM LiCl, and root growth over the next three days was measured. E) HPTS fluorescence ratios around root cells of indicated genotypes. Data are pooled from measurements taken on three different days, each normalized to the average of wild-type values on those days. Graphs show means ± s.d. Letters in graphs indicate statistical classes based on Tukey’s Honestly Significant Difference test. n, from left to right: panel B: 28, 24, 24, 30, 26, 27, 26, 25, 26, 25, 24, 20, 24, 26, 25; panel D: 21, 27, 16, 23, 19, 25, 20, 30, 18, 28, 21, 30, 23, 31, 18, 29; panel E: 26, 13, 14, 16., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311117
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311117
HANDLE: http://hdl.handle.net/10261/311117
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311117
PMID: http://hdl.handle.net/10261/311117
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311117
Ver en: http://hdl.handle.net/10261/311117
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oai:digital.csic.es:10261/311117

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311125
Dataset. 2022

LOCALIZATION OF SAUR63:YFP:HA IN ROOT MERISTEM EPIDERMAL CELLS COMPARED TO CONTROL WAVE LINES

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
A-D)P35S:SAUR63:YFP:HA. E-H)P35S:SAUR6326-142:YFP:HA. I-L)PUBQ10:WAVE 138Y expressing a PM-localized YFP fusion protein. M-P) PUBQ10:WAVE 1Y expressing a cytoplasmically-localized YFP fusion protein. Q-T) PUBQ10:WAVE 9Y expressing a YFP fusion protein localized to the tonoplast. Shown are fluorescence confocal microscopy images of YFP (green, A,E,I,M,Q), FM4-64 membrane staining (magenta, B,F,J,N,R), and both channels together (C,G,K,O,S) with vertical yellow lines indicating locations of quantitation of fluorescence intensity signals, scaled to the maximum signal along the line (D,H,L,P,T). Image color channel brightnesses were adjusted for visibility. Scale bar, 20 μm., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311125
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311125
HANDLE: http://hdl.handle.net/10261/311125
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311125
PMID: http://hdl.handle.net/10261/311125
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311125
Ver en: http://hdl.handle.net/10261/311125
Digital.CSIC. Repositorio Institucional del CSIC
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