Resultados totales (Incluyendo duplicados): 34416
Encontrada(s) 3442 página(s)
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311172
Dataset. 2022

ANTI-INFLAMMATORY AND NEUROPROTECTIVE EVALUATION OF DIVERSE MICROALGAE EXTRACTS ENRICHED IN CAROTENOIDS: APPENDIX A. SUPPLEMENTARY DATA

  • Gallego, Rocío
  • Valdés, Alberto
  • Suárez Montenegro, Zully J.
  • Sánchez-Martínez, J. David
  • Cifuentes, Alejandro
  • Ibáñez, Elena
  • Herrero, Miguel
Supplementary figures., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311172
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311172
HANDLE: http://hdl.handle.net/10261/311172
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311172
PMID: http://hdl.handle.net/10261/311172
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311172
Ver en: http://hdl.handle.net/10261/311172
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311172

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311174
Dataset. 2022

SUPPLEMENTARY MATERIALS FOR ANTIOXIDANT POTENTIAL OF THE SWEET WHEY-BASED BEVERAGE COLADA AFTER THE DIGESTIVE PROCESS AND RELATIONSHIPS WITH THE LIPID AND PROTEIN FRACTIONS

  • García-Casas, Victoria E.
  • Seiquer, Isabel
  • Pardo, Zaira
  • Haro, Ana
  • Recio, Isidra
  • Olías, Raquel
The supporting information: Table S1, Discriminant sensory analysis of different formulations of Colada; Table S2, Hedonic evaluation of Colada (15% Maracuyá juice) by consumers. Figure S1. Chromatogram (GC) of fatty acid methyl esters (FAME) of the whey-beverage (Colada) before the in vitro digestion. Figure S2. Chromatogram (GC) of fatty acid methyl esters (FAME) of the whey-beverage (Colada) after the in vitro digestion (bioaccessible fraction). Figure S3. Chromatogram (HPLC) of amino acids of the whey-beverage (Colada) (acid hydrolysis). Figure S4. Chromatogram (HPLC) of amino acids of the whey-beverage (Colada) (performic oxidation), Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311174
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311174
HANDLE: http://hdl.handle.net/10261/311174
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311174
PMID: http://hdl.handle.net/10261/311174
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311174
Ver en: http://hdl.handle.net/10261/311174
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311174

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311182
Dataset. 2022

VISUALIZATION OF SAUR63:YFP:HA FUSION PROTEIN VARIANTS IN COTYLEDONS

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
1 figure., A-M) Confocal images showing YFP fluorescence of transgenic lines expressing the indicated fusion proteins behind the P35S promoter. Scale bar, 20 μm., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311182
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311182
HANDLE: http://hdl.handle.net/10261/311182
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311182
PMID: http://hdl.handle.net/10261/311182
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311182
Ver en: http://hdl.handle.net/10261/311182
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311182

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311185
Dataset. 2022

SUPPLEMENTARY MATERIALS FOR IN VITRO DIGESTIBILITY AND BIOACCESSIBILITY OF NUTRIENTS AND NON-NUTRIENTS COMPOSING EXTRUDED BREWERS’ SPENT GRAIN

  • Gutiérrez-Barrutia, María Belén
  • Cozzano Ferreira, Sonia
  • Arcia, Patricia
  • Castillo, M. Dolores del
The following supporting information: Figure S1: IEC-6 viability for DBSG and DEBSG. *shows significant differences with positive control (C+) (p < 0.05). Figure S2: IEC-6 viability for ferulic acid. Figure S3: RAW 264.7 viability for DBSG and DEBSG. *shows significant differences with positive control (C+) (p < 0.05). Figure S4: RAW264.7 viability for ferulic acid. Figure S5: Maltose released from potato starch due to α-amylase activity of rat intestinal extract. Figure S6: p-Nitrophenyl released due to α-glucosidase activity of rat intestinal extract. Figure S7: Glucose released from sucrose due to sucrose activity of rat intestinal extract., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311185
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311185
HANDLE: http://hdl.handle.net/10261/311185
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311185
PMID: http://hdl.handle.net/10261/311185
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311185
Ver en: http://hdl.handle.net/10261/311185
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311185

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311190
Dataset. 2022

LOCALIZATION OF SAUR63:YFP:HA VARIANTS IN NICOTIANA BENTHAMIANA LEAF CELLS

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
1 figure., A-L) Confocal images showing YFP fluorescence of indicated SAUR63:YFP:HA variants expressed in transiently transformed N. benthamiana leaves. Scale bar, 20 μm., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311190
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311190
HANDLE: http://hdl.handle.net/10261/311190
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311190
PMID: http://hdl.handle.net/10261/311190
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311190
Ver en: http://hdl.handle.net/10261/311190
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311190

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311194
Dataset. 2022

EFFECTS OF N-TERMINAL ALTERATIONS ON PROTEIN LEVELS AND FRACTIONATION OF SAUR63:YFP:HA FUSION PROTEINS

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
1 figure., A-E) Protein levels in multiple P35S:SAUR63:YFP:HA, P35S:SAUR6326-142:YFP:HA, and P35S:CBL11-12:SAUR6326-142:YFP:HA pooled T2 seedlings from different T1 transformants. The blot in panel E shows protein extracts from selected genotypes in panels A-D for side-by-side comparison in the same experiment. F,G) SAUR63:YFP:HA, SAUR6326-142:YFP:HA, SAUR631-25:YFP:HA, CBL11-12:SAUR6326-142:YFP:HA, and SAUR63m2:YFP:HA fusion proteins, detected by western blots in total (T), soluble (S) and microsomal (M) protein fractions. Lower panels show controls for loading or fractionation. α-HA detects SAUR63 fusion protein; α-AHA2 detects a membrane protein; α-UGPase and α-APX detect soluble proteins. Arrows indicate position of full-sized SAUR63:YFP:HA fusion proteins. FL, Full-length SAUR63:YFP:HA fusion protein. wt, wild-type Columbia lacking any transgene. In genotype designations, S63 is short for SAUR63. Letters and numbers after genotype designations indicate independent transgenic lines. H) Amount of SAUR63:YFP:HA and SAUR6326-142:YFP:HA proteins in whole seedling extracts at indicated times after start of cycloheximide treatment to block new protein synthesis. Fusion proteins were detected by western blots using anti-HA antibody. The Rubisco large subunit band from Ponceau S staining of the same gels is shown as a loading control. A repeat of this experiment gave a very similar result. In the lower gel, the larger band is the presumed intact SAUR6326-142:YFP:HA protein, and the lower band is a presumed smaller breakdown product. In genotype labels, S63 denotes SAUR63, letters and numbers after genotype names indicate distinct transgenic lines, FL denotes a strong full-length P35S:SAUR63:YFP:HA line used as a common standard line in most experiments, and wt indicates wild-type Columbia lacking any transgene., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311194
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311194
HANDLE: http://hdl.handle.net/10261/311194
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311194
PMID: http://hdl.handle.net/10261/311194
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311194
Ver en: http://hdl.handle.net/10261/311194
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311194

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311195
Dataset. 2022

POLLUTION INDUCES EPIGENETIC EFFECTS THAT ARE STABLY TRANSMITTED ACROSS MULTIPLE GENERATIONS [DATASET]

  • Harney, Ewan
  • Paterson, Steve
  • Collin, Hélène
  • Chan, Brian H.K.
  • Bennett, Daimark
  • Plaistow, Stewart J.
[Methods] Please refer to the article "Pollution induces epigenetic effects that are stably transmitted across multiple generations" for information about methods. [Usage notes] Please refer to the README file for information about data and R scripts., It has been hypothesised that the effects of pollutants on phenotypes can be passed to subsequent generations through epigenetic inheritance, affecting populations long after the removal of a pollutant. But there is still little evidence that pollutants can induce persistent epigenetic effects in animals. Here we show that low doses of commonly used pollutants induce genome-wide differences in cytosine methylation in the freshwater crustacean Daphnia pulex. Uniclonal populations were either continually exposed to pollutants or switched to clean water, and methylation was compared to control populations that did not experience pollutant exposure. While some direct changes to methylation were only present in the continually exposed populations, others were present in both the continually exposed and switched to clean water treatments, suggesting that these modifications had persisted for seven months (> 15 generations). We also identified modifications which were only present in the populations that had switched to clean water, indicating a long-term legacy of pollutant exposure distinct from the persistent effects. Pollutant-induced differential methylation tended to occur at sites that were highly methylated in controls. Modifications that were observed in both continually and switched treatments were highly methylated in controls and showed reduced methylation in the treatments. On the other hand, modifications found just in the switched treatment tended to have lower levels of methylation in the controls and showed increase methylation in the switched treatment. In a second experiment we confirmed that sub-lethal doses of the same pollutants generate effects on life-histories for at least three generations following the removal of the pollutant. Our results demonstrate that even low doses of pollutants can induce transgenerational epigenetic effects that are stably transmitted over many generations. Persistent effects are likely to influence phenotypic development, which could contribute to the rapid adaptation, or extinction, of populations confronted by anthropogenic stressors., NERC, Award: NE/I024437/1. Natural Environment Research Council, Award: NE/N016017/1., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311195
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311195
HANDLE: http://hdl.handle.net/10261/311195
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311195
PMID: http://hdl.handle.net/10261/311195
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311195
Ver en: http://hdl.handle.net/10261/311195
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311195

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311200
Dataset. 2022

SAUR63 FUSION PROTEIN LIPID BINDING

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
1 figure., A) Western blot showing presence of fusion proteins in extracts used in lipid blot experiments in Fig 5A. Arrows indicate locations of full-length SAUR63:YFP:HA and truncated SAUR6326-142:YFP:HA fusion proteins (upper arrow) and SAUR631-25:YFP:HA N-terminal domain fusion protein (lower arrow). B) Longer exposures of two lipid blots from Fig 5A. C) Mock experiment in which extracts were incubated for 70 minutes in protein extraction buffer at the indicated temperatures, and then run on a gel for western blots. For both SAUR63:YFP:HA and SAUR6326-142:YFP:HA, similar amounts of protein are present after incubation at -20 C or after incubation at 22 C, as during the lipid blot binding experiment., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311200
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311200
HANDLE: http://hdl.handle.net/10261/311200
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311200
PMID: http://hdl.handle.net/10261/311200
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311200
Ver en: http://hdl.handle.net/10261/311200
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311200

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311205
Dataset. 2022

PEST:SAUR63NAAIRS:CERFP:HA LINES

  • Nagpal, Punita
  • Reeves, Paul H.
  • Wong, Jeh Haur
  • Armengot, Laia
  • Chae, Keun
  • Rieveschl, Nathaniel B.
  • Trinidad, Brendan
  • Davidsdottir, Vala
  • Jain, Prateek
  • Gray, William M.
  • Jaillais, Yvon
  • Reed, Jason W.
1 figure., A)PEST:SAUR63NAAIRS:CerFP:HA lines grown on plates with estradiol (all genotypes) or without estradiol (wild-type Columbia and PEST:SAUR63:CerFP:HA only). S2 Table indicates amino acids changed in each mutant and root tortuosity index measurement data from this and one replicate experiment. Scale bar, 1 cm. B) Western blots of total protein in estradiol-induced PEST:SAUR63NAAIRS:CerFP:HA lines using α-HA antibody., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311205
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311205
HANDLE: http://hdl.handle.net/10261/311205
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311205
PMID: http://hdl.handle.net/10261/311205
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311205
Ver en: http://hdl.handle.net/10261/311205
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311205

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311206
Dataset. 2022

SUPPLEMENTARY MATERIALS FOR MICROWAVE-ASSISTED ACID HYDROLYSIS VS. CONVENTIONAL HYDROLYSIS TO PRODUCE SAPOGENIN-RICH PRODUCTS FROM FENUGREEK EXTRACTS

  • Navarro del Hierro, Joaquín
  • Cantero-Bahillo, Emma
  • Fernández-Felipe, M. Teresa
  • Martín, Diana
The supporting information: Figure S1: Sapogenin content (g/100 g) and extraction yield (%) of extracts obtained at different molarities of HCl by microwave hydrolysis at 140 °C for 30 min. Standard deviations are indicated by error bars. Mean values with different letters are significantly different (p ≤ 0.05); Figure S2: Lipase inhibition (%) and sapogenin content (g/100 g) of extracts obtained at different molarities of HCl by microwave hydrolysis at 140 °C for 30 min. Standard deviations are indicated by error bars. Mean values with different letters are significantly different (p ≤ 0.05); Figure S3: DPPH inhibition (%) and sapogenin content (g/100 g) of extracts obtained at different molarities of HCl by microwave hydrolysis at 140 °C for 30 min. Standard deviations are indicated by error bars. Mean values with different letters are significantly different (p ≤ 0.05); Table S1: Sapogenin profile of sapogenin-rich extracts produced from a commercial saponin-rich fenugreek extract under conventional hydrolysis (100 °C, 60 min) or microwave-assisted acid hydrolysis (MAAH, 30 min) at different temperatures (100, 120, 130 and 140 °C); Table S2: Sapogenin profile of sapogenin-rich extracts produced from a commercial saponin-rich fenugreek extract under conventional hydrolysis (100 °C, 60 min) or microwave-assisted acid hydrolysis (MAAH, 140 °C) at different times (10, 20, 30 and 40 min)., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/311206
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311206
HANDLE: http://hdl.handle.net/10261/311206
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311206
PMID: http://hdl.handle.net/10261/311206
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311206
Ver en: http://hdl.handle.net/10261/311206
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/311206

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