Resultados de investigación

9 pages. -- Table S1: Average values of physical and biogeochemical parameters defining each cluster during spring and neap tides. Mean, N, standard deviation (SD) and range. -- Fig. S1: Average values of physical and biogeochemical variables defining each cluster during spring and neap tides. Purple bars represent CL1, green bars for CL2. -- Fig. S2: Picoplankton groups biomass distribution. Synechococcus (A-B), Prochlorococcus (C-D) and Cryptophytes (E-F) bio-mass (mgC m-3) during spring (A, C, E) and neap tides (B, D, F). -- Fig. S3: Main microplankton groups biomass (mgC m-3) distribution during spring (A, C, E, G) and neap (B, D, F, H) tides. -- Table S2. Main pico and nanoplankton groups cell densities (cell mL-1) and biomass. -- Table S3. Microplankton abundance (cell mL-1) and biomass (mgC m-3)by major groups during neap and spring tides. -- Table S4. Mesoplankton abundance (ind m-3) and biomass (mgC m-3) by major groups during neap and spring tides. -- Table S5. Summary scheme signing main features defining each cluster. -- Table S6. Total abundance of copepods orders (ind m-3). -- Fig. S4: Mean temperature, (A) and N2 (B) profiles averaged for all the stations. The dashed lines represent the 20th and 80th percentiles in both plots., Peer reviewed

Appendix S1. Summary of random encounter model published studies. Appendix S2. Study areas and reference methods details. Appendix S3. Assessing improvements in precision., Peer reviewed

Table S1: Number of alleles per locus in the recent past and current periods for Dupont´s lark; Table S2: Observed allele frequencies at each locus in each region at both periods of time (left = recent past; right = current). The number of individuals typed is shown in brackets. Person´s r correlation values (R) between allele frequencies in each period within each region are also indicated. Rare alleles are in bold and private alleles at each period are underlined; Table S3: Paired t-test results for temporal variation of genetic diversity (observed heterozygosity, Ho; unbiased expected heterozygosity, uHe; allelic richness, Ar; and inbreeding, FIS) of Dupont´s lark males at country (Morocco, Spain) and regional level. Significant values are represented with an asterisk; Table S4: Genetic diversity estimates for each of the nine Dupont´s lark regions considering the samples that overlapped geographically (sampled at the same localities) during the recent past (P) and the current (C) period. Sample size (N), observed heterozygosity (Ho), unbiased expected heterozygosity (uHe), allelic richness (AR), inbreeding coefficient (FIS). Paired t-test results for temporal variation of genetic diversity (observed heterozygosity, Ho; unbiased expected heterozygosity, uHe; allelic richness, AR and inbreeding, FIS) at regional level are also shown. Significant values are represented with an asterisk; Table S5: Matrix of pairwise (GST and D) values between regions in Morocco (Anti Atlas, AA; Ain Bni Mathar, ABM; Midelt, MID; Plateau of Rekkam, REKK). Values below diagonal correspond to comparisons in the recent past period and above diagonal in the current period. Values among sampling periods within regions are shown on the diagonal. Asterisks indicate statistically significant values; Table S6: Matrix of pairwise (GST and D) values between regions in Spain (Ebro Valley, EV; Iberian Mountains, IM; Northern Plateau, NP; Southern Plateau, SP and Southern Spain, SS). Values below diagonal correspond to comparisons in the recent past period and above diagonal in the current period. Values among sampling periods within regions are shown on the diagonal. Asterisks indicate statistically significant values., Peer reviewed

3 pages. -- Figure S1: Atmospheric conditions during rain ION period, the 29 May 2017. -- Figure S2: Atmopsheric conditions during rain FAST period, the 05 June 2017, Peer reviewed

Supplementary Material, Appendix 1., Peer reviewed

Table S1. Number of individuals sampled according to species, age and sex. Table S2. Capture systems used for sampling each species included in our active monitoring study to assess avian scavenger exposure to anticoagulant rodenticides in NE Spain. Table S3. MS/MS dMRM transition parameters, recovery and limits of quantification (LOQ) for the analysed SGARs and the internal standard (IS). Table S4. Prevalence (%) and concentrations (ng mL-1)1 of the ΣSGARs concentration > LOQ (i.e., considering only individuals with SGAR levels > LOQ) in the blood of free-living obligate and facultative avian scavengers from the northwest Iberian Peninsula according to age class (nestling, juvenile, subadult and adult). Fig. S1. Percentage of birds containing residues of more than one SGARs in the blood. Regarding SGAR-positive birds (n = 102), we found more than one compound in 48 (47.1%) of the birds. Of these, 31 birds (64.6%) presented with two different compounds; 16 (33.3%) presented with three compounds and one bird (2.1%) presented with four different SGARs. Of all SGAR-positive birds showing more than one SGAR in the blood, 81.2% were Egyptian vultures (n = 25; 58.14% of all), red kites (n = 10; 40%) and black kites (n = 4; 50%). BF (Brodifacoum), DF (Difenacoum), FL (Flocoumafen), BM (Bromadiolone)., Peer reviewed

6 pages. -- Table S1. Coordinates of sampling sites and related Meteorological stations. -- Table S2. Summary of the available metabolic data per species, region and season. -- Table S3. Summary of statistical analyses, performance of mixed models with random factor “publication”, summary of fixed effects through additional ANOVA analysis. -- Figure S1. Metabolic rates (CR, NCP and GPP in mmol O2 m-2 day-1) in relationship with the in situ temperature in the Posidonia oceanica benthic chambers data (top plot) and for all sensor data (bottom plot) in the Western basin. -- Figure S2 A. Seasonal metabolic rates (GPP, NCP and CR in mmol O2 m-2 day-1) in the Western Mediterranean basin calculated with sensor data. -- Figure S2 B. Seasonal metabolic rates (GPP, NCP and CR in mmol O2 m-2 day-1) calculated with benthic chamber data for Posidonia oceanica. -- Figure S3. Changes over time (Year) in metabolic rates (GPP, NCP and CR in mmol O2 m-2 day-1) for A) rates calculated with sensor data (both basins) and B) incubations of Posidonia oceanica., Peer reviewed

Fig. S1. Conceptual model of avoidance. A primary repellent evokes reflexive withdrawal immediately after exposure. A secondary repellent is avoided because an animal associates a conditional stimulus (sound, sight, taste) to an aversive experience (e.g., illness, pain) with a sensory stimulus. A generalized conditional aversion may occur against the food that previously contained the chemical and it is avoided by only the type of food as the cue to refusing it. Fig. S2. Diagram of test of avoidance to treated sorghum seeds. Fig. S3. Diagram of experiment of avoidance to soybean seeds treated with imidacloprid. Fig. S4. Diagram of experiment of avoidance to soybean cotyledons treated with neonicotinoids. Fig. S5. Frequency of signs of intoxication observed over the days in each treatment during the Exposure 1 (A) and Exposure 2 (B) periods of the sorghum experiment. Signs of intoxication were measured as present or absent at each moment of observation, consisting of from lethargy to motionless. Letters indicate significant differences among treatments. IMI: red bars, CLO: green bars, THI: blue bars. Fig. S6. Body weights of survivors and dead in the sorghum experiment. The deaths only occurred in the sorghum experiment, during the Exposure 1 period, in birds treated with imidacloprid (3 dead) and clothianidin (1 dead). In the dead, the BWs were measured at the time of death. In survivors, BWs were measured at the end of the Exposure 1 period. Table S1. Records of maximum and minimum temperature and humidity in each period, and average daily thermal amplitude. Table S2. Doves that consumed soybeans (12 of the 40). Consumption was measured from the fourth day of the "Pre-Exposure 1" period. The number of seeds consumed was calculated based on the average daily weight of soybeans used to measure the humidity factor. Table S3. Average mass in grams of seeds and cotyledons. "Ad Sur" sorghum is the AD75STA commercial hybrid sorghum of "adSur, Agrosemillas del Sur SA", used in COM, IMI and THI treatments of the sorghum test. “DK” sorghum is the “Dekalb” commercial hybrid sorghum seeds of Syngenta®, used in CLO treatment of the sorghum test. Table S4. Average consumption in grams (mean ± SE) of test food and the total consumption (grams of test food + grams of maintenance food) during periods of exposure to neonicotinoids. In the sorghum seeds, CON is the untreated sorghum control and COM is the commercial sorghum control. Table S5. Video records of the behavior of some doves during the first 15 minutes of exposure to food in the different treatments and periods. “1rt peck” indicates the time in seconds when the bird first pecked at the food inside the feeder after it was offered; “n peck food” is the number of times the bird pecked at the feeder food during the first 15 minutes of its exposure to the food; “n grit peck” is the number of times the bird pecked at the grit; “n drink” is the number of times the bird drank water from the drinking fountain; “n grooming” is the number of times the bird groomed itself; “n shaking” is the number of times the bird shook; “on perch” is the number of times the bird climbed on the perch. In the sorghum experiment, CON is the untreated sorghum control and COM is the commercial sorghum control. Table S6. Body condition index (SMI) and the p-value of the paired Wilcoxon test used to compare the initial and final body conditions of each treatment in the three experiments. In the sorghum experiment, CON is the untreated sorghum control and COM is the commercial sorghum control., Peer reviewed

6 pages. -- Supplementary Table S1.Results of the 3-way PERMANOVA for isotopes (δ13C and δ15N), C and N content and C/N ratio in the mussel Mytilus galloprovincialis for the monitoring experiment. -- Supplementary Table S2. Average and standard deviation in δ13C, δ15N, carbon and nitrogen content and C/N ratio of pooled mussels’ tissues of Mytilus galloprovincialis infected and uninfected with the African pea crab Afropinnotheres monodi. -- Supplementary Table S3. Results of the 3-way (Model 2) and 1-way (Model 3) PERMANOVA for isotopes (δ13C and δ15N), C and N content and C/N ratio in the mussel Mytilus galloprovincialis for the monitoring experiment. -- Supplementary Table S4. Average and standard deviation in δ13C, δ15N, carbon and nitrogen content and C/N ratio of pooled mussels’ tissues of Mytilus galloprovincialis and the African pea crab Afropinnotheres monodi after 30 days reared under ad libitum food (Day 30) and subsequently exposed to no access to food during 21 days (Day 51). -- Supplementary text: Isotopic variability of the symbiont and the host species. -- Supplementary Figure S1. Relationships between the carbon and nitrogen isotopic signal (‰) of Afropinnotheres monodi and the muscle of the host bivalve species Mytilus galloprovincialis (locations 2 and 3) and Scrobicularia plana (location 1) in the three locations studied., Peer reviewed

13 pages. -- This PDF file includes: Figures S1 to S7 and Tables S1 to S2. -- Fig. S1. Characterization of scFab-apoferritin fusions. -- Fig. S2. Multabody affinity-purification scheme. Protein A and Protein L sequential affinity purification. -- Fig. S3. Generation of a Multabody that cross-targets the HIV-1 Env and the CD4 receptor. -- Fig. S4. Biophysical characterization of HIV-1 Multabodies. Comparison of the Tm and Tagg temperatures of T-01/T-02 MB, 12-mer ferritin fusions, parental IgGs and the N6/PGDM1400x10E8v4 trispecific antibody. -- Fig. S5. Binding characteristics of IgGs binding to four different antigens. -- Fig. S6. Multabody v2 features. -- Fig. S7. PsV neutralization and inhibition of primary PBMC infection by Multabodies. -- Table S1. IC50 of individual and IgG mixtures, PGDM1400/N6x10E8v4 trispecific and HIV-1 Multabodies against a 14-PsV panel and 25-PsV panel (additional 11 HIV-1 strains highly resistant to PGDM1400). -- Table S2. Potency of parental and IgG mixtures and T-01 Multabody versions against a 118-PsV panel., Peer reviewed