Resultados totales (Incluyendo duplicados): 33743
Encontrada(s) 3375 página(s)
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331731
Dataset. 2022

ADDITIONAL FILE 8 OF A COARSE-GRAINED APPROACH TO MODEL THE DYNAMICS OF THE ACTOMYOSIN CORTEX [DATASET]

  • Hernández del Valle, Miguel
  • Valencia-Expósito, Andrea
  • López-Izquierdo, Antonio
  • Casanova-Ferrer, Pau
  • Tarazona, Pedro
  • Martín-Bermudo, María D.
  • Míguez, David G.
Additional file 8: Movie S4. Oscillations in D. Melanogaster basal follicle cells. Control conditions. Cells are stained with Lifeact-GFP., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331731
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331731
HANDLE: http://hdl.handle.net/10261/331731
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331731
PMID: http://hdl.handle.net/10261/331731
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331731
Ver en: http://hdl.handle.net/10261/331731
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331731

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331741
Dataset. 2023

IFISC ANNUAL REPORT 2022

  • CSIC-UIB - Instituto de Física Interdisciplinar y Sistemas Complejos (IFISC)
IFISC (Institute for Cross-Disciplinary Physics and Complex Systems) is a joint research institute of the University of the Balearic Islands (UIB) and the Spanish National Research Council (CSIC) created in 2007 building upon the former Cross-Disciplinary Physics Department of the Mediterranean Institute for Advanced Studies (IMEDEA) dating from 1995. Its mission is to develop Cross-Disciplinary and Strategic Research in Complex Systems following the established scientific approach of physicists., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331741
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331741
HANDLE: http://hdl.handle.net/10261/331741
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331741
PMID: http://hdl.handle.net/10261/331741
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331741
Ver en: http://hdl.handle.net/10261/331741
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331741

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331746
Dataset. 2022

IMMUNOBLOT ANALYSIS OF PEDEM::GFP EXPRESSION IN YOUNG ANIMALS

  • Ghenea, Simona
  • Chiritoiu, Marioara
  • Tacutu, Robi
  • Miranda-Vizuete, Antonio
  • Petrescu, Stefana Maria
S1 Fig. Immunoblot analysis of Pedem::GFP expression in young animals. (A) Quantitative real-time PCR (qPCR) of edem-3 mRNA. WT and edem-3 mutant worms treated with empty vector or edem-3 RNAi; expression were normalized to that of cdc-42 and pmp-3. (B) Total protein lysates derived from WT Pedem::GFP transgenic animals subjected to the indicated treatment were separated by SDS-PAGE and immunoblotted with anti-GFP polyclonal antisera; tubulin was used as loading control. NS- non-treated, TM- tunicamycin, HS-heat stress, OS- osmotic stress. The histograms show the densitometry values of Pedem::GFP bands normalized to the value of of NS condition (n=3 ± SEM, t test), *P<0.05; **P<0.01; ***P<0.001; ns, not significant. (C) RNAi downregulation of edem triggered accumulation of CPL-1* in intestinal cells. To overrule a significant contribution of autofluorescent stress granules to the GFP fluorescence, images captured with Diode laser were included. Scale bar: 20 μm., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331746
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331746
HANDLE: http://hdl.handle.net/10261/331746
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331746
PMID: http://hdl.handle.net/10261/331746
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331746
Ver en: http://hdl.handle.net/10261/331746
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331746

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331748
Dataset. 2023

SPEIBASE_COUNTRIES

  • Vicente Serrano, Sergio M.
  • Beguería, Santiago
  • Reig-Gracia, Fergus
[EN] It contains a collection of .csv files by country and a netCDF file. Last one need specific data analyse software. [ES] Contiene una colección de archivos .csv por país y un archivo netCDF. Este último necesita software de análisis de datos específico., [EN] This database includes the representative Standardized Precipitation Evapotranspiration Index (SPEI) series for the different countries of the world from 1901 at the time scales from 1 to 48 months. The data is based on the average precipitation and reference evapotranspiration series from the Climatic Research Unit (last version) for the different world countries., [ES] Esta base de datos incluye la serie representativa del Índice Estandarizado de Precipitación y Evapotranspiración (SPEI) para los diferentes países del mundo desde 1901 en las escalas de tiempo de 1 a 48 meses. Los datos se basan en las series de precipitación media y evapotranspiración de referencia de la Unidad de Investigación Climática (última versión) para los diferentes países del mundo., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331748
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331748
HANDLE: http://hdl.handle.net/10261/331748
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331748
PMID: http://hdl.handle.net/10261/331748
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331748
Ver en: http://hdl.handle.net/10261/331748
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331748

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751
Dataset. 2022

EDEM-1 AND EDEM-2 ARE REQUIRED FOR CPL-1* DEGRADATION

  • Ghenea, Simona
  • Chiritoiu, Marioara
  • Tacutu, Robi
  • Miranda-Vizuete, Antonio
  • Petrescu, Stefana Maria
S2 Fig. EDEM-1 and EDEM-2 are required for CPL-1* degradation (A) Fluorescence intensities of WT animals carrying the CPL-1* transgene treated with indicated concentrations of kifunensine. Values in scatter gram represent mean fluorescence/µm2 x 1000 in arbitrary units (AU). The red bars indicate the average ±SEM. ****P<0.0001; one way ANOVA with Dunnett post test. (B) Immunoblot analysis of CPL-1* degradation in edem-1 and edem-2 mutants carrying the rescuing EDEM-1::mCherry and EDEM-2::mCherry transgenes, respectively. Total protein lysates derived from edem-1 and edem-2 mutants carrying the CPL-1* and rescuing transgenes were separated by SDS-PAGE and immunoblotted with anti-GFP polyclonal antisera. Tubulin was used as loading control. (C) Histogram showing the densitometry values of the bands presented in (B) normalized to the value of WT condition (n=3 ± SEM, one way ANOVA with Fisher test), *P<0.05; **P<0.01)., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751
HANDLE: http://hdl.handle.net/10261/331751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751
PMID: http://hdl.handle.net/10261/331751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751
Ver en: http://hdl.handle.net/10261/331751
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331751

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331755
Dataset. 2022

EDEM DEPLETION MITIGATES ER STRESS

  • Ghenea, Simona
  • Chiritoiu, Marioara
  • Tacutu, Robi
  • Miranda-Vizuete, Antonio
  • Petrescu, Stefana Maria
S3 Fig. EDEM depletion mitigates ER stress. (A) Representative confocal images of WT(EV) control or indicated RNAi depleted young adults carrying the Phsp-4::GFP transgene under non ER-stress conditions (upper panel), treatment with 5 μg/ml tunicamycin for 12h (middle panel), and heat shock, and 5 μM thapsigargin treatment (lower panel). TM-tunicamycin, HS-heat shock, TG-thapsigargin. The worms were outlined for better visualization. Images were obtained using the same confocal settings. Exposure adjustments were uniformly applied. Scale bar: 50 μm. (B) Quantitative RT-PCR measurements of Phsp-4::mRNA levels in indicated strains under non stress and treatment with 5 μg/ml tunicamycin (n = 3 independent experiments). (C) Quantitative RT-PCR measurements of Phsp-4::mRNA levels in indicated strains under non stress and heat stress treatment (n = 3 independent experiments). In (B) and (C) quantifications were normalized relative to WT non stress conditions. ****P<0.0001, ns, not significant. Quantifications were normalized relative to WT non stress conditions. (D) Representative confocal images of WT control and edem-2 mutants carrying the PC12C8.1::GFP transgene under non ER-stress conditions. The left panels show fluorescence images and the right panels DIC images., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331755
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331755
HANDLE: http://hdl.handle.net/10261/331755
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331755
PMID: http://hdl.handle.net/10261/331755
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331755
Ver en: http://hdl.handle.net/10261/331755
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331755

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331758
Dataset. 2022

EDEM RESPONSE TO THAPSIGARGIN

  • Ghenea, Simona
  • Chiritoiu, Marioara
  • Tacutu, Robi
  • Miranda-Vizuete, Antonio
  • Petrescu, Stefana Maria
S4 Fig. edem response to thapsigargin. (A) Quantitative RT-PCR measurements of edem mRNA levels in WT young animals under non stress (NS) and treatment with 5 μM thapsigargin (TG), (n = 3 independent experiments). (B) Percentage of eggs that developed into L4 larvae after 3 days on 10 μM thapsigargin. Each strain was scored in three independent experiments in triplicates. (C) Quantification of WT (EV) or RNAi-treated day one adults carrying Phsp-4::GFP transgene, treated or not with 5 μM thapsigargin. The worms were kept on RNAi plates for one generation before exposure to ER stress treatments. Values represent mean fluorescence/µm2 x 1000. The red bars indicate the average ±SEM. (D) Analysis of xbp-1 spliced/unspliced ratio from young adults of indicated strains. Total RNA was isolated, reverse transcribed and used for PCR analysis of xbp-1 splicing forms., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331758
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331758
HANDLE: http://hdl.handle.net/10261/331758
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331758
PMID: http://hdl.handle.net/10261/331758
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331758
Ver en: http://hdl.handle.net/10261/331758
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331758

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331760
Dataset. 2022

STRAINS USED IN THIS STUDY. TARGETING EDEM PROTECTS AGAINST ER STRESS AND IMPROVES DEVELOPMENT AND SURVIVAL IN C. ELEGANS

  • Ghenea, Simona
  • Chiritoiu, Marioara
  • Tacutu, Robi
  • Miranda-Vizuete, Antonio
  • Petrescu, Stefana Maria
S1 Table. Strains used in this study., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331760
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331760
HANDLE: http://hdl.handle.net/10261/331760
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331760
PMID: http://hdl.handle.net/10261/331760
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331760
Ver en: http://hdl.handle.net/10261/331760
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331760

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331764
Dataset. 2022

PRIMERS USED IN THIS STUDY. TARGETING EDEM PROTECTS AGAINST ER STRESS AND IMPROVES DEVELOPMENT AND SURVIVAL IN C. ELEGANS

  • Ghenea, Simona
  • Chiritoiu, Marioara
  • Tacutu, Robi
  • Miranda-Vizuete, Antonio
  • Petrescu, Stefana Maria
S2 Table. Primers used in this study., Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331764
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331764
HANDLE: http://hdl.handle.net/10261/331764
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331764
PMID: http://hdl.handle.net/10261/331764
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331764
Ver en: http://hdl.handle.net/10261/331764
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331764

Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331766
Dataset. 2022

ADDITIONAL FILE 9 OF A COARSE-GRAINED APPROACH TO MODEL THE DYNAMICS OF THE ACTOMYOSIN CORTEX [DATASET]

  • Hernández del Valle, Miguel
  • Valencia-Expósito, Andrea
  • López-Izquierdo, Antonio
  • Casanova-Ferrer, Pau
  • Tarazona, Pedro
  • Martín-Bermudo, María D.
  • Míguez, David G.
Additional file 9: Movie S5. Oscillations in D. Melanogaster basal follicle cells. Experimental conditions. Time-lapse movie of the D. Melanogaster basal follicle cells stained with Lifeact-GFP in conditions of over-expression of the two subunits of the integrin molecule., Ministerio de Ciencia, Innovación y Universidades, Peer reviewed

Proyecto: //
DOI: http://hdl.handle.net/10261/331766
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331766
HANDLE: http://hdl.handle.net/10261/331766
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331766
PMID: http://hdl.handle.net/10261/331766
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331766
Ver en: http://hdl.handle.net/10261/331766
Digital.CSIC. Repositorio Institucional del CSIC
oai:digital.csic.es:10261/331766

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