BUSCADOR RECOLECTA

(A–A”) Schematic drawings of an S9 egg chamber illustrating the point of ablation (blue bar) on the basal (A’) and apical (A”) side of a FC. NCs are in gray, BCs in yellow, FCs in purple, and BM in blue. (B, E, H, K) Images of the basal (B, E) and apical (H, K) sides of life S9 egg chambers of the indicated genotypes before and after FC bonds are ablated. Blue bars indicate points of ablation. (C, F, I, L) Quantification over time of initial velocity of vertex displacement and (D, G, J, M) vertex displacement of the indicated ablated bonds. The statistical significance of differences was assessed with a t test, * P value < 0.05, ** P value < 0.01, and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in B, E, H, and K, 5 μm. The raw data underlying panels C, D, F, G, I, J, L, and M are available in S1 Data. BC, border cell; BM, basement membrane; FC, follicle cell; NC, nurse cell., Peer reviewed

[Description of methods used for collection/generation of data] Individual capture hsitories were built from long-term individual capture-recapture data of adult Scopoli’s shearwater Calonectris diomedea breeding in Pantaleu islet. Some of these individuals were also equipped with geoloctaors, that when recovered may provide information of individual wintering areas. All the information about the deployment of geolocators devices and wintering areas visited are encoded in the individual capture histories.The file inlcudes 23 annual encounter occasions, which translate into 45 coded encounter occasions, with the ‘inter’ encounter occasions encoding information about presence in the colony and migration decisions, and the ‘intra’ occasions encoding information about whether a geolocator had been deployed or not at the end of the season., No utilizar sin el previo consentimiento de los propietarios., Este fichero contiene información de las historias de captura individuales de Pardela cenicienta criando en el islote de Pantaleu, Mallorca, durante el período 2000-2022. En estas historias de captura anuales se ha incluido información de la colocación de dispositivos geolocalizadores en algunos de estos individuos, y la información que se obtuvo de las zonas de invernada visitadas por estos individuos., Peer reviewed

(A) Schematic drawings of an S9 egg chamber illustrating the point of ablation (blue bar). NCs are in gray, BCs in yellow, FCs in purple, and BM in blue. (B) Images of life S9 control (tjGal4) and tj>LanB1RNAi egg chambers expressing Resille-GFP before and after NC bonds are ablated. Blue bars indicate points of ablation. (C) Quantification of initial velocity of vertex displacement and (D) vertex displacement over time of the indicated ablated bonds. (E) Schematic drawing of an S9 egg chamber, similar to the one shown in A, illustrating the mirrorGal4 pattern of expression (mirrGal4, pink). (F, I) Life images of NCs located in the area anterior to (F) and at the (I) the mirr expressing region of S9 control (mirrGal4) and mirr>EHBP1mCh egg chambers expressing Resille-GFP before and after NC bonds are ablated. Blue bars indicate points of ablation. (G, J) Quantification over time of initial velocity of vertex displacement and (H, K) vertex displacement over time of the indicated ablated bonds. The statistical significance of differences was assessed with a t test, * P value < 0.05 and *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in B, F, and I, 10 μm. The raw data underlying panels C, D, G, H, J, and K are available in S1 Data. BC, border cell; BM, basement membrane; FC, follicle cell; GFP, green fluorescent protein; NC, nurse cell., Peer reviewed

(A–D) Stills taken from live imaging of BCs from egg chambers of the designated genotypes. The blue circles indicate the BC cluster bodies. (E, F) Mean number of protrusions observed per frame (snapshots) in each direction from BC clusters of egg chambers of the indicated genotypes. (G–J’) Stills taken from live imaging of BC clusters from egg chambers of the designated genotypes. The pink asterisks mark the BC being followed. White arrows indicate the direction of the movement. (K–M) Quantification of BC cluster rotation. The statistical significance of differences was assessed with a t test, * P value < 0.05. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A–D and G–J, 10 μm. The raw data underlying panels E, F and K–M are available in S1 Data. BC, border cell; BM, basement membrane., Peer reviewed

(A–B”) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. (C, D) Quantification of BC migration speed in egg chambers of the indicated genotypes in the area anterior to (C) and at the (D) mirr expressing region. (E) Quantification of egg chambers with complete BC migration. The statistical significance of differences was assessed with a t test, * P value < 0.05. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A” and B”, 20 μm. The raw data underlying panels C–E are available in S1 Data. BC, border cell; FC, follicle cell., Peer reviewed

(A) Position of ovaries within a Drosophila female (left) and schematic of Drosophila ovaries showing an ovariole and its egg chambers (right). BC, border cells; Oo, oocyte; NCs, nurse cells; FCs, follicle cells; StFCs, stretch FCs; BM, basement membrane. (B) Diagram of an ovariole showing stages 8, 9, and 10 egg chambers (S8–S10). Anterior is left, posterior right. (C) Three-dimensional diagram of a S9 egg chamber showing the migration of the BCs between the NCs. (D–G’) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. Discontinuous yellow lines demarcate the region between the anterior border of the egg chamber and the oocyte anterior membrane. (H–J) Quantification of BC migration speed during the whole migratory process (H) and during the early (I) and late (J) phases in egg chambers of the indicated genotypes. The statistical significance of differences was assessed with a t test, ** P value < 0.01. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A’, B’, C’ and D’, 20 μm. The raw data underlying panels H–J are available in S1 Data., Peer reviewed

Suppl. Figure 1. Percentage of FES patients in symptomatic remission throughout the study., Suppl. Figure 2. Percentage of FES patients in functional remission throughout the study., Suppl. Figure 3. Clustered boxplot displaying the mean depression scores per time point (measured using the Hamilton Depression Rating Scale) in the subgroup meeting criteria for a depressive episode at baseline (assessed through the SCID-I) versus the subgroup without a depressive episode at baseline., Suppl. Table 1. Baseline demographics and clinical characteristics of study completers and drop-outs., Suppl. Table 2. Number of patients in symptomatic and functional remission throughout the period of follow-up., Suppl. Table 3. Generalized linear mixed models: pairwise contrasts for symptomatic and functional remission., Suppl. Table 4. Linear mixed models: estimated marginal means for PANSS, GAF, SOFAS, CGI, GF-S, GF-R and HAM-D., Suppl. Table 5. Linear mixed models: estimates of fixed effect time on PANSS, GAF, SOFAS, CGI, GF-S, GF-R and HAM-D., Suppl. Table 6. Number of patients admitted to the hospital since the previous visit due to psychotic symptoms and due to psychiatric reasons in general., Suppl. Table 7. Substances used at least once in the past three months (WHO-ASSIST)., Suppl. Table 8. Number of patients reporting daily or almost daily substance use in the past three months, separated per time point and substance category (WHO-ASSIST)., Suppl. Table 9. Number of patients reporting daily or almost daily substance use in the past three months, separated for the group of symptomatic remitters and non-remitters at month 12., Suppl. Table 10. Baseline demographics and clinical characteristics of symptomatic remitters and non-remitters at month 12., Suppl. Table 11. Summary of sociodemographics and baseline clinical characteristics of FES participants, separated per country., Suppl. Table 12. Number of FES patients in symptomatic remission throughout the period of follow-up, separated per country., Suppl. Table 13. Number of FES patients in functional remission throughout the period of follow-up, separated per country., Suppl. Table 14. Number of FES patients admitted to the hospital for psychiatric reasons throughout the period of follow-up, separated per country., Suppl. Table 15. Number of FES patients admitted to the hospital for psychosis throughout the period of follow-up, separated per country., Suppl. Table 16. Number of FES patients using antipsychotic medication throughout the period of follow-up, separated per country., Suppl. Table 17. Overview of subjects not meeting eligibility criteria., Suppl. Table 18. Number of patients in symptomatic and functional remission throughout the period of follow-up when using alternative definitions., Suppl. Table 19. Generalized linear mixed models: pairwise contrasts for symptomatic and functional remission when using alternative definitions., Peer reviewed

The basement membrane (BM) is a specialized extracellular matrix (ECM), which underlies or encases developing tissues. Mechanical properties of encasing BMs have been shown to profoundly influence the shaping of associated tissues. Here, we use the migration of the border cells (BCs) of the Drosophila egg chamber to unravel a new role of encasing BMs in cell migration. BCs move between a group of cells, the nurse cells (NCs), that are enclosed by a monolayer of follicle cells (FCs), which is, in turn, surrounded by a BM, the follicle BM. We show that increasing or reducing the stiffness of the follicle BM, by altering laminins or type IV collagen levels, conversely affects BC migration speed and alters migration mode and dynamics. Follicle BM stiffness also controls pairwise NC and FC cortical tension. We propose that constraints imposed by the follicle BM influence NC and FC cortical tension, which, in turn, regulate BC migration. Encasing BMs emerge as key players in the regulation of collective cell migration during morphogenesis., Peer reviewed

(A–B’) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. (C) Quantification of the migration defects in egg chambers of the specified genotypes. The statistical significance of differences was assessed with a t test, *** P value < 0.001. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A’ and B’, 20 μm. The raw data underlying panel C are available in S1 Data., Peer reviewed

(A–D) S9 controls tjGal4, tj>LanB1RNAi, mirrGal4 and mirr>EHBP1mCh egg chambers stained with the F-actin marker Rhodamine-Phalloidin (F-actin, red) and the DNA marker Hoechst (blue). Arrows in A indicate the curvature of the membranes between an anterior (A) and an anterior central NC (Ca); between 2 central NCs -a Ca and a Cp (a posterior central NC); between a Cp and a posterior (P) NC and between a P NC and the oocyte membrane. (E, F) Quantification of the radius of curvature of A, Ca, Cp and P NCs in S9 egg chambers of the indicated genotypes. BC clusters are marked with a red circle. (G–I) Stills taken from live imaging of migrating BCs from egg chambers of the indicated genotypes. (J) Quantification of BC migration speed in egg chambers of the indicated genotypes. The statistical significance of differences was assessed with a t test. Horizontal and vertical lines indicate mean and SD, respectively. Scale bars in A–D and G–I’, 20 μm. The raw data underlying panels E, F, and J are available in S1 Data., Peer reviewed