BUSCADOR RECOLECTA

Supplemental Reference 1: Articles reporting EML for medical screening of EHRs, included in the analysis., Peer reviewed

Supplemental Reference 2: Articles reporting on ML models for medical screening of EHRs but without EML, excluded from the analysis., Peer reviewed

Supplemental Table 1. Type of studies in the selected articles, Peer reviewed

The upper part of the panel shows the genomic organization of PepMV. RdRp: RNA dependent RNA polymerase gene; TGBs: Triple gene block protein-encoding genes; CP: coat protein gene. The lower part of the panel shows the alignment of the CP region including the Cys127 codon after genotyping a PepMV-infected plant after one passage (#9), and the 8 PepCPC127S-infected plants after one passage (#1–8). The Cys127 codon is framed with a red rectangle. The Cys127 replacement was stable in all the individual passages, Peer reviewed

Supplemental Table 2. Inclusion Exclusion criteria of diseases and their classification by medical specialties, Peer reviewed

Western blot of a protein extract from N. benthamiana leaves expressing PepCPWT. Protein extract aliquots were oxidized with CuCl2 (lanes 1 and 2), reduced with dithiothreitol (lanes 3 and 4), or not pre-treated with any of them (lanes 5–6). Protein extracts loaded in lanes 2, 4 and 6 were incubated with methyl-polyethylene glycol-maleimide (MM(PEG)24) as described by Pant et al. (2021). Reduced cysteines are alkylated with MM(PEG)24 and one conjugation increases the protein mass by 1.24 kDa. A double band was observed in lanes 2 and 4, indicating partial CP oxidation, reduction or alkylation in the controls. The membrane was stripped and re-probed with an anti-Actin antibody as the loading control, Peer reviewed

Western blot of a protein extract from N. benthamiana leaves expressing PepCPWT. Protein extract aliquots were oxidized with CuCl2 (lanes 1 and 2), reduced with dithiothreitol (lanes 3 and 4), or not pre-treated with any of them (lanes 5–6). Protein extracts loaded in lanes 2, 4 and 6 were incubated with methyl-polyethylene glycol-maleimide (MM(PEG)24) as described by Pant et al. (2021). Reduced cysteines are alkylated with MM(PEG)24 and one conjugation increases the protein mass by 1.24 kDa. A double band was observed in lanes 2 and 4, indicating partial CP oxidation, reduction or alkylation in the controls. The membrane was stripped and re-probed with an anti-Actin antibody as the loading control, Peer reviewed

Coomassie-stained SDS-PAGE gel of protein extractions from Nicotiana benthamiana leaves expressing the CP wild type (CP) or the CP mutant CPC127S. EV: pJL-TRBO empty vector; M: Marker. CP and CPC127S are expressed at similar levels, Peer reviewed

[Introduction] Tissue engineering has emerged as an innovative approach to treat critical-size bone defects using biocompatible scaffolds, thus avoiding complex distraction surgeries or limited stock grafts. Continuous regeneration monitoring is essential in critical-size cases due to the frequent appearance of non-unions. This work evaluates the potential clinical use of gait analysis for the mechanical assessment of a tissue engineering regeneration as an alternative to the traditional and hardly conclusive manual or radiological follow-up., [Materials and methods] The 15-mm metatarsal fragment of eight female merino sheep was surgically replaced by a bioceramic scaffold stabilized with an external fixator. Gait tests were performed weekly by making the sheep walk on an instrumented gangway. The evolution of different kinematic and dynamic parameters was analyzed for all the animal’s limbs, as well as asymmetries between limbs. Finally, potential correlation in the recovery of the gait parameters was evaluated through the linear regression models., [Results] After surgery, the operated limb has an altered way of carrying body weight while walking. Its loading capacity was significantly reduced as the stance phases were shorter and less impulsive. The non-operated limbs compensated for this mobility deficit. All parameters were normalizing during the consolidation phase while the bone callus was simultaneously mineralizing. The results also showed high levels of asymmetry between the operated limb and its contralateral, which exceeded 150% when analyzing the impulse after surgery. Gait recovery significantly correlated between symmetrical limbs., [Conclusions] Gait analysis was presented as an effective, low-cost tool capable of mechanically predicting the regeneration of critical-size defects treated by tissue engineering, as comparing regeneration processes or novel scaffolds. Despite the progressive normalization as the callus mineralized, the bearing capacity reduction and the asymmetry of the operated limb were more significant than in other orthopedic alternatives., Peer reviewed

OBJ of the geometry of the scaffold designed from CT scans of an intermediate 13-mm ovine metatarsal bone fragment. The specific modifications made to the scaffold for stability and regenerative response are included., Peer reviewed